BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0172500 Os12g0172500|AK101515
         (563 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0172500  WD40-like domain containing protein                 986   0.0  
Os11g0176000  WD40-like domain containing protein                 521   e-148
Os10g0104500  Similar to Notchless-related                         68   2e-11

>Os12g0172500 WD40-like domain containing protein
          Length = 563

 Score =  986 bits (2549), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 483/540 (89%), Positives = 483/540 (89%)

Query: 24  LKTYFKTPEGRYKLQYEKTHSAAVLHYNHGGKTVSQLTVAYLKEKPAGQXXXXXXXXXXX 83
           LKTYFKTPEGRYKLQYEKTHSAAVLHYNHGGKTVSQLTVAYLKEKPAGQ           
Sbjct: 24  LKTYFKTPEGRYKLQYEKTHSAAVLHYNHGGKTVSQLTVAYLKEKPAGQGSTPSTPSSSS 83

Query: 84  XMRSAAARLLGSGNGNRTLSFVGGNGVSRAVXXXXXXXXXXXXXXXXXXXQAVANYDGKG 143
            MRSAAARLLGSGNGNRTLSFVGGNGVSRAV                   QAVANYDGKG
Sbjct: 84  GMRSAAARLLGSGNGNRTLSFVGGNGVSRAVSGSSRIGGGLGTSGGIGSSQAVANYDGKG 143

Query: 144 TYIIFNTADTLFISDLNSQDKDPIKSIHFSNSNPLCHAFDPEAKEGHDLIIGMGSGDVYS 203
           TYIIFNTADTLFISDLNSQDKDPIKSIHFSNSNPLCHAFDPEAKEGHDLIIGMGSGDVYS
Sbjct: 144 TYIIFNTADTLFISDLNSQDKDPIKSIHFSNSNPLCHAFDPEAKEGHDLIIGMGSGDVYS 203

Query: 204 MSLRQQLQDSGRKPVAAQHYNKGDKDGTSTGSRCTSVAWVPEREGIFVVSHSDGNLYVYD 263
           MSLRQQLQDSGRKPVAAQHYNKGDKDGTSTGSRCTSVAWVPEREGIFVVSHSDGNLYVYD
Sbjct: 204 MSLRQQLQDSGRKPVAAQHYNKGDKDGTSTGSRCTSVAWVPEREGIFVVSHSDGNLYVYD 263

Query: 264 KCKDGNTECTFPAIKDPAQLMISHAKSSKSNPIARWHVCQGSINAISFSPDGAYLATVGR 323
           KCKDGNTECTFPAIKDPAQLMISHAKSSKSNPIARWHVCQGSINAISFSPDGAYLATVGR
Sbjct: 264 KCKDGNTECTFPAIKDPAQLMISHAKSSKSNPIARWHVCQGSINAISFSPDGAYLATVGR 323

Query: 324 DGYLRVFDFSKEQLIFGGKSYYGALLCCTWSSDGKYLLTGGEDDLVQVWSMDDRKIVAWG 383
           DGYLRVFDFSKEQLIFGGKSYYGALLCCTWSSDGKYLLTGGEDDLVQVWSMDDRKIVAWG
Sbjct: 324 DGYLRVFDFSKEQLIFGGKSYYGALLCCTWSSDGKYLLTGGEDDLVQVWSMDDRKIVAWG 383

Query: 384 EGHNSWVSGVSFDSYWSPPNSDGTGENTVYRFGSVGQDTQLLLWDLALDEIAVPLRHXXX 443
           EGHNSWVSGVSFDSYWSPPNSDGTGENTVYRFGSVGQDTQLLLWDLALDEIAVPLRH   
Sbjct: 384 EGHNSWVSGVSFDSYWSPPNSDGTGENTVYRFGSVGQDTQLLLWDLALDEIAVPLRHPSS 443

Query: 444 XXXXXXXXXXXAHWDNACLPTGVLQPSPRMRDVPKLSPLVAHRVHVDPLSGLVFTNESIL 503
                      AHWDNACLPTGVLQPSPRMRDVPKLSPLVAHRVHVDPLSGLVFTNESIL
Sbjct: 444 GSPTFSSGSPSAHWDNACLPTGVLQPSPRMRDVPKLSPLVAHRVHVDPLSGLVFTNESIL 503

Query: 504 TICREGLIKIWVRPDXXXXXXXXXXXXLVLGNPVSKDRVITTSNKATGSSFKQSSAVVFT 563
           TICREGLIKIWVRPD            LVLGNPVSKDRVITTSNKATGSSFKQSSAVVFT
Sbjct: 504 TICREGLIKIWVRPDQNENNHQSNSSELVLGNPVSKDRVITTSNKATGSSFKQSSAVVFT 563



>Os11g0176000 WD40-like domain containing protein
          Length = 522

 Score =  521 bits (1341), Expect = e-148,   Method: Compositional matrix adjust.
 Identities = 277/502 (55%), Positives = 330/502 (65%), Gaps = 68/502 (13%)

Query: 60  LTVAYLKEKPAGQXXXXXXXXXXXXMRSAAARLLGSGNGNRTLSFVGGNGVSRAVXXXXX 119
           +TVAYLKE P+              MRS AARLLG+G G R LSFVG NGV         
Sbjct: 1   MTVAYLKEMPS----TPSIPSCTSGMRSVAARLLGAGTGTRPLSFVGSNGVG-------- 48

Query: 120 XXXXXXXXXXXXXXQAVANYDG-KGTYIIFNTADTLFISDLNSQDKDPIKSIHFSNSNPL 178
                         +A+ NYD  +GTYIIFN AD+LFI DLN   + P+K I FS+  PL
Sbjct: 49  -GRSASGSCHAGQSRALVNYDDDRGTYIIFNIADSLFIRDLNY--RRPVKRICFSDMKPL 105

Query: 179 CHAFDPEAKEGHDLIIGMGSGDVYSMSLRQQLQDSGRKPVAAQHYNKGDKDGTSTGSRCT 238
           CHAFD EAK+GHDLI+G  SG+VYSMSLRQQL++ G +P+A QH+        +T SRCT
Sbjct: 106 CHAFDSEAKDGHDLIVGFLSGEVYSMSLRQQLKEPGPEPIALQHF-------FNTNSRCT 158

Query: 239 SVAWVPEREGIFVVSHSDGNLYVYDKCKDGNTECTFPAIKDPAQLMISHAKSSKSNPIAR 298
            VAWVP  EG FVVS++DGNL+VYDK KD NT+ TFP ++D +++ IS+AKSSKSNP+AR
Sbjct: 159 GVAWVPGHEGFFVVSNADGNLFVYDKSKDVNTDWTFPTVEDQSEMKISYAKSSKSNPVAR 218

Query: 299 WHVCQGSINAISFSPDGAYLATVGRDGYLRVFDFSKEQLIFGGKSYYGALLCCTWSSDGK 358
           WH+CQG+INAISFSPDG YLAT+GRDGYLRVFDF+KEQLIFGGKSY+GALLCC+WS+DGK
Sbjct: 219 WHICQGAINAISFSPDGTYLATIGRDGYLRVFDFAKEQLIFGGKSYFGALLCCSWSTDGK 278

Query: 359 YLLTGGEDDLVQVWSMDDRKIVAWGEGHNSWVSGVSFDSYWSP----------------- 401
           YLL+GGEDDLVQVWSM DRK+VAWGEGH SWVS V+FDSYWSP                 
Sbjct: 279 YLLSGGEDDLVQVWSMHDRKMVAWGEGHKSWVSAVAFDSYWSPPKPYERKQNSMHRFASP 338

Query: 402 ---------------PNSDGTGENT--VYRFGSVGQDTQLLLWDLALDEIAVPLRHXXXX 444
                          P SD T ENT  +YRF S+GQD QLLLWDL  DE+ V L H    
Sbjct: 339 KSDEAEEDPIYSFASPKSDETKENTNIMYRFASIGQDAQLLLWDLTKDELNVSLTHASSC 398

Query: 445 XXXXXXXXXXAHWD---------NACLPTGVLQPSPRMRDVPKLSPLVAHRVHVDPLSGL 495
                     A            +   P G L PSPR+++VPKLSP VAH V V+PL  L
Sbjct: 399 SESSSSGSCSASSSSGSSSTEDRDKEFPLGFLHPSPRLQEVPKLSPEVAHLVGVEPLFTL 458

Query: 496 VFTNESILTICREGLIKIWVRP 517
            FT+ES++T+CR G  +I  RP
Sbjct: 459 EFTSESVITVCRRG--RITTRP 478



>Os10g0104500 Similar to Notchless-related
          Length = 480

 Score = 67.8 bits (164), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 50/189 (26%), Positives = 82/189 (43%), Gaps = 26/189 (13%)

Query: 258 NLYVYDKCKDGNTECTFPAIKDPAQLMISHAKSSKSNPIARWHVCQGSIN-------AIS 310
            L  Y + K+ N E T   +  P  +        +  P+ R   C  +I        A+S
Sbjct: 73  QLGAYMQQKNANVEVTLRIVYQPQAIF-------RIRPVNR---CSATIAGHTEAVLAVS 122

Query: 311 FSPDGAYLATVGRDGYLRVFDFSKEQLIFGGKSYYGALLCCTWSSDGKYLLTGGEDDLVQ 370
           FSPDG  LA+   D  +R +D S +  +F  K +   +LC  WS DG +L++G +   + 
Sbjct: 123 FSPDGRCLASGSGDTTVRFWDLSTQTPLFTCKGHKNWVLCIAWSPDGNHLVSGSKSGELI 182

Query: 371 VWSMDDRKIVAWG-EGHNSWVSGVSFDSYWSPPNSDGTGENTVYRFGSVGQDTQLLLWDL 429
           +W     K +     GH  W++ VS    W P +     ++   RF S  +D    +WD+
Sbjct: 183 LWDPKTGKQLGTPLTGHRKWITAVS----WEPVHL----QSPCRRFVSTSKDGDARIWDM 234

Query: 430 ALDEIAVPL 438
              +  + L
Sbjct: 235 TTRKCVIAL 243



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.134    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 19,035,313
Number of extensions: 835499
Number of successful extensions: 2069
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 2078
Number of HSP's successfully gapped: 4
Length of query: 563
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 457
Effective length of database: 11,501,117
Effective search space: 5256010469
Effective search space used: 5256010469
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 159 (65.9 bits)