BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0149000 Os12g0149000|AK108734
         (359 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0149000  Conserved hypothetical protein                      186   3e-47
Os11g0150100  Protein of unknown function DUF868, plant fami...    77   3e-14

>Os12g0149000 Conserved hypothetical protein
          Length = 359

 Score =  186 bits (471), Expect = 3e-47,   Method: Compositional matrix adjust.
 Identities = 98/126 (77%), Positives = 98/126 (77%)

Query: 13  VRDFASCIGDGAACTAPSSTRTRRGSAAVQAQPSTLSVTASYRVXXXXXXXXXXQLRLTW 72
           VRDFASCIGDGAACTAPSSTRTRRGSAAVQAQPSTLSVTASYRV          QLRLTW
Sbjct: 13  VRDFASCIGDGAACTAPSSTRTRRGSAAVQAQPSTLSVTASYRVALASSSSPPLQLRLTW 72

Query: 73  AHSPLGPTLSFSPSASGRKVLVRRRRGSCSVLXXXXXXXXXXXXXXXXXXPRLALFWDLT 132
           AHSPLGPTLSFSPSASGRKVLVRRRRGSCSVL                  PRLALFWDLT
Sbjct: 73  AHSPLGPTLSFSPSASGRKVLVRRRRGSCSVLSSGEDEAVESESELSASSPRLALFWDLT 132

Query: 133 AARFDP 138
           AARFDP
Sbjct: 133 AARFDP 138



>Os11g0150100 Protein of unknown function DUF868, plant family protein
          Length = 338

 Score = 76.6 bits (187), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 64/143 (44%), Positives = 71/143 (49%), Gaps = 17/143 (11%)

Query: 13  VRDFASCIGDGA-------ACTAPSSTRTRRGSAAVQAQPSTLS----VTASYRVXXXXX 61
           VRDFASCIGDGA       ACTAPSSTR    S++     S+ S    VT SYR      
Sbjct: 13  VRDFASCIGDGAVRVADVAACTAPSSTRVSTCSSSSSTTASSSSPTLSVTVSYRATLLAA 72

Query: 62  XXXXXQLRLTWAHSPLGPTLSFSPSASGRKV------LVRRRRGSCSVLXXXXXXXXXXX 115
                QLRLTW HSPLGPTLSF+PS + R +        R      S             
Sbjct: 73  PAPPLQLRLTWGHSPLGPTLSFAPSPTARAIQLRRRRGSRSLPSGSSSGDENGGGGDESG 132

Query: 116 XXXXXXXPRLALFWDLTAARFDP 138
                  P LALFWDLTAAR+DP
Sbjct: 133 TTTPPPPPPLALFWDLTAARYDP 155



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.130    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,324,506
Number of extensions: 99885
Number of successful extensions: 350
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 346
Number of HSP's successfully gapped: 2
Length of query: 359
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 257
Effective length of database: 11,709,973
Effective search space: 3009463061
Effective search space used: 3009463061
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 157 (65.1 bits)