BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0133900 Os12g0133900|AK102656
         (542 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0133900  Similar to Epa4p                                    685   0.0  
Os11g0137100  Similar to U3 small nucleolar ribonucleoprotei...    96   7e-20

>Os12g0133900 Similar to Epa4p
          Length = 542

 Score =  685 bits (1768), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 371/542 (68%), Positives = 371/542 (68%)

Query: 1   MAMDLDEPLDAEKGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKHLYASLVPFSPAQPP 60
           MAMDLDEPLDAEKGE                             KHLYASLVPFSPAQPP
Sbjct: 1   MAMDLDEPLDAEKGEAALRRLRDADPALYLSPSADLAAAAREASKHLYASLVPFSPAQPP 60

Query: 61  PLSNLLAGPAFDAEQIWSQIELLSRPLIPHLRRQLRRLEKQPPSQPPPRTXXXXXXXXXX 120
           PLSNLLAGPAFDAEQIWSQIELLSRPLIPHLRRQLRRLEKQPPSQPPPRT          
Sbjct: 61  PLSNLLAGPAFDAEQIWSQIELLSRPLIPHLRRQLRRLEKQPPSQPPPRTESKSADAEEK 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNQVEDEFFKIKDLD 180
                                                         NQVEDEFFKIKDLD
Sbjct: 121 SEEEDEEDGEGDEELEELDDVDDEEESEEEEEEEEEDKEGLEDKAGNQVEDEFFKIKDLD 180

Query: 181 KFMVXXXXXXXXXXXXXXXXXXXTENWMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           KFMV                   TENWM                                
Sbjct: 181 KFMVKGEEAEYGGGAKQGKKKKKTENWMEEDSDEEEEEDLDEDEDNEDEEDDDEHLDLED 240

Query: 241 XXXXXXXXXGAVGDIMYKDFFEEADDQKVRKKGGSKKVQFXXXXXXXXXXXXXXXGNDEQ 300
                    GAVGDIMYKDFFEEADDQKVRKKGGSKKVQF               GNDEQ
Sbjct: 241 FDDEEEEEEGAVGDIMYKDFFEEADDQKVRKKGGSKKVQFKDEPDEPEVDDKNDDGNDEQ 300

Query: 301 GLSTHEKARLKMHAKIEEMEKANLEPSMWTMQGEVNASSRPKNSALEVDLDFEHNVRPAP 360
           GLSTHEKARLKMHAKIEEMEKANLEPSMWTMQGEVNASSRPKNSALEVDLDFEHNVRPAP
Sbjct: 301 GLSTHEKARLKMHAKIEEMEKANLEPSMWTMQGEVNASSRPKNSALEVDLDFEHNVRPAP 360

Query: 361 VITEEVTASLEEMIKKRIIEGHFDDVEKPSPLQFKSPKEQKDLDESKSKKGLAELYEDDY 420
           VITEEVTASLEEMIKKRIIEGHFDDVEKPSPLQFKSPKEQKDLDESKSKKGLAELYEDDY
Sbjct: 361 VITEEVTASLEEMIKKRIIEGHFDDVEKPSPLQFKSPKEQKDLDESKSKKGLAELYEDDY 420

Query: 421 AQKAGLAPAPLSISDELKNEANTLFKRICLKLDALSHFHFAPKPVIEDMSIQANVPALAM 480
           AQKAGLAPAPLSISDELKNEANTLFKRICLKLDALSHFHFAPKPVIEDMSIQANVPALAM
Sbjct: 421 AQKAGLAPAPLSISDELKNEANTLFKRICLKLDALSHFHFAPKPVIEDMSIQANVPALAM 480

Query: 481 EEIAPVAVSDAAMLAPEEIFEGKGDVKEEAELTQAEXXXXXXXXXXXYAGSHKERPAKMQ 540
           EEIAPVAVSDAAMLAPEEIFEGKGDVKEEAELTQAE           YAGSHKERPAKMQ
Sbjct: 481 EEIAPVAVSDAAMLAPEEIFEGKGDVKEEAELTQAERRRRRANKKRRYAGSHKERPAKMQ 540

Query: 541 KD 542
           KD
Sbjct: 541 KD 542



>Os11g0137100 Similar to U3 small nucleolar ribonucleoprotein protein MPP10 (M
           phase phosphoprotein 10)
          Length = 74

 Score = 95.9 bits (237), Expect = 7e-20,   Method: Composition-based stats.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 335 VNASSRPKNSALEVDLDFEHNVRPAPVITEEVTASLEEMIKKRIIE 380
           VNASSRPKNSALEVDLDFEHNVRPAPVITEEVTASLEEMIKKRIIE
Sbjct: 1   VNASSRPKNSALEVDLDFEHNVRPAPVITEEVTASLEEMIKKRIIE 46



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.130    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,745,849
Number of extensions: 519494
Number of successful extensions: 1371
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1370
Number of HSP's successfully gapped: 3
Length of query: 542
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 436
Effective length of database: 11,501,117
Effective search space: 5014487012
Effective search space used: 5014487012
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 158 (65.5 bits)