BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os12g0109200 Os12g0109200|AK103380
         (128 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os12g0109200  Similar to Ca(2+)-dependent nuclease                181   1e-46
Os11g0109500  Staphylococcus nuclease (SNase-like) domain co...   115   1e-26

>Os12g0109200 Similar to Ca(2+)-dependent nuclease
          Length = 128

 Score =  181 bits (459), Expect = 1e-46,   Method: Compositional matrix adjust.
 Identities = 93/128 (72%), Positives = 93/128 (72%)

Query: 1   MGNILRCFKXXXXXXXHYPYYKPTSRXXXXXXXXXXXXXXXXXXXXXXXXXXXXVTPSTV 60
           MGNILRCFK       HYPYYKPTSR                            VTPSTV
Sbjct: 1   MGNILRCFKGDDDGGDHYPYYKPTSRPHYQPPHYHGQPAAPPAPPQQQPLGPHGVTPSTV 60

Query: 61  GVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWAFLNFTTFRSHHSPQHRQTTNHPR 120
           GVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWAFLNFTTFRSHHSPQHRQTTNHPR
Sbjct: 61  GVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWAFLNFTTFRSHHSPQHRQTTNHPR 120

Query: 121 YQRACSLC 128
           YQRACSLC
Sbjct: 121 YQRACSLC 128



>Os11g0109500 Staphylococcus nuclease (SNase-like) domain containing protein
          Length = 311

 Score =  115 bits (287), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 61/96 (63%), Positives = 61/96 (63%)

Query: 1  MGNILRCFKXXXXXXXHYPYYKPTSRXXXXXXXXXXXXXXXXXXXXXXXXXXXXVTPSTV 60
          MGNILRCFK       HYPYYKPTSR                            VTPSTV
Sbjct: 1  MGNILRCFKGDDDGGDHYPYYKPTSRPHYQPPHYHGQPAAPPAPLQQQHLGPHGVTPSTV 60

Query: 61 GVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKW 96
          GVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKW
Sbjct: 61 GVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKW 96



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.131    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,229,818
Number of extensions: 84807
Number of successful extensions: 250
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 248
Number of HSP's successfully gapped: 2
Length of query: 128
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 39
Effective length of database: 12,388,755
Effective search space: 483161445
Effective search space used: 483161445
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 150 (62.4 bits)