BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os11g0149400 Os11g0149400|AK063900
         (119 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os11g0149400  Phytosulfokines 2 precursor [Contains: Phytosu...   112   7e-26
Os12g0147800  Phytosulfokines 5 precursor (Secretory protein...    68   2e-12

>Os11g0149400 Phytosulfokines 2 precursor [Contains: Phytosulfokine-alpha (PSK-
           alpha) (Phytosulfokine-a)
          Length = 119

 Score =  112 bits (279), Expect = 7e-26,   Method: Compositional matrix adjust.
 Identities = 64/119 (53%), Positives = 64/119 (53%)

Query: 1   MSTTRGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVPRDEHQENGGVKXXXXXXXXXX 60
           MSTTRGV                              VPRDEHQENGGVK          
Sbjct: 1   MSTTRGVSSSSAAAALALLLLFALCFFSFHFAAAARAVPRDEHQENGGVKAVAAVAADQL 60

Query: 61  XXXXEGDTGNGDEVSELMGXXXXXXXXXXXGKNNDECVQRRLLSDAHLDYIYTQHKNKP 119
               EGDTGNGDEVSELMG           GKNNDECVQRRLLSDAHLDYIYTQHKNKP
Sbjct: 61  VLQLEGDTGNGDEVSELMGAAEEEAAACEEGKNNDECVQRRLLSDAHLDYIYTQHKNKP 119



>Os12g0147800 Phytosulfokines 5 precursor (Secretory protein SH27A) [Contains:
           Phytosulfokine-alpha (PSK-alpha) (Phytosulfokine-a)
          Length = 102

 Score = 67.8 bits (164), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 32/55 (58%), Positives = 39/55 (70%), Gaps = 1/55 (1%)

Query: 65  EGDTGNGDEVSELMGXXXXXXXXXXXGKNNDECVQRRLLSDAHLDYIYTQHKNKP 119
           +G TGNGDEVSELMG            + N+ECV+RR+L DAHLDYIYTQ +N+P
Sbjct: 49  DGATGNGDEVSELMGAAEEEAAGLCE-EGNEECVERRMLRDAHLDYIYTQKRNRP 102



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.309    0.132    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,447,731
Number of extensions: 63436
Number of successful extensions: 20
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 2
Length of query: 119
Length of database: 17,035,801
Length adjustment: 85
Effective length of query: 34
Effective length of database: 12,597,611
Effective search space: 428318774
Effective search space used: 428318774
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 149 (62.0 bits)