BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os10g0545700 Os10g0545700|AK074009
(160 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os10g0545700 Similar to Dual specificity phosphatase Cdc25 ... 297 2e-81
Os03g0108000 Similar to Dual specificity phosphatase Cdc25 ... 203 4e-53
>Os10g0545700 Similar to Dual specificity phosphatase Cdc25 (EC 3.1.3.48) (Arath
Length = 160
Score = 297 bits (761), Expect = 2e-81, Method: Compositional matrix adjust.
Identities = 145/160 (90%), Positives = 145/160 (90%)
Query: 1 MCRFLISTPFSXXXXXXXXXXXXXXXSVSYVSAAKLLAMARSNPRVAIIDVRDEERSYQA 60
MCRFLISTPFS SVSYVSAAKLLAMARSNPRVAIIDVRDEERSYQA
Sbjct: 1 MCRFLISTPFSRRRGERKAEAGRMARSVSYVSAAKLLAMARSNPRVAIIDVRDEERSYQA 60
Query: 61 HIGGSHHFSSRSFAARLPELARATGDKDTVVFHCALSKVRGPSCAKMFSDYLSETKEESG 120
HIGGSHHFSSRSFAARLPELARATGDKDTVVFHCALSKVRGPSCAKMFSDYLSETKEESG
Sbjct: 61 HIGGSHHFSSRSFAARLPELARATGDKDTVVFHCALSKVRGPSCAKMFSDYLSETKEESG 120
Query: 121 TKNIMVLERGFNGWELSGQPVCRCTDAPCKGTCSPEEPEL 160
TKNIMVLERGFNGWELSGQPVCRCTDAPCKGTCSPEEPEL
Sbjct: 121 TKNIMVLERGFNGWELSGQPVCRCTDAPCKGTCSPEEPEL 160
>Os03g0108000 Similar to Dual specificity phosphatase Cdc25 (EC 3.1.3.48) (Arath
Length = 146
Score = 203 bits (517), Expect = 4e-53, Method: Compositional matrix adjust.
Identities = 94/127 (74%), Positives = 109/127 (85%), Gaps = 1/127 (0%)
Query: 28 VSYVSAAKLLAMARSNPRVAIIDVRDEERSYQAHIGGSHHFSSRSFAARLPELARATGDK 87
VSYVSAA+L+ M R +PR+A++DVRDEER Y AHI GSHH++S SF RLPELA+AT K
Sbjct: 21 VSYVSAAQLVPMLR-DPRIAVVDVRDEERIYDAHIAGSHHYASDSFGERLPELAQATKGK 79
Query: 88 DTVVFHCALSKVRGPSCAKMFSDYLSETKEESGTKNIMVLERGFNGWELSGQPVCRCTDA 147
+T+VFHCALSKVRGPSCA+M+ DYLSE E+S KNIMVLERGFNGWELSG+PVCRC DA
Sbjct: 80 ETLVFHCALSKVRGPSCAQMYLDYLSEADEDSDVKNIMVLERGFNGWELSGRPVCRCKDA 139
Query: 148 PCKGTCS 154
PCKG CS
Sbjct: 140 PCKGVCS 146
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.131 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,942,186
Number of extensions: 172999
Number of successful extensions: 377
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 376
Number of HSP's successfully gapped: 2
Length of query: 160
Length of database: 17,035,801
Length adjustment: 93
Effective length of query: 67
Effective length of database: 12,179,899
Effective search space: 816053233
Effective search space used: 816053233
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 152 (63.2 bits)