BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0502600 Os10g0502600|AK069511
         (232 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0502600  Cytochrome b5 domain containing protein             252   1e-67
Os02g0640300  Similar to Membrane steroid binding protein 1 ...   177   7e-45
Os10g0502500  Cytochrome b5 domain containing protein             165   3e-41
Os03g0321000  Similar to Steroid membrane binding protein-like    140   1e-33
Os02g0793700  Cytochrome b5 domain containing protein             112   3e-25

>Os10g0502600 Cytochrome b5 domain containing protein
          Length = 232

 Score =  252 bits (644), Expect = 1e-67,   Method: Compositional matrix adjust.
 Identities = 132/178 (74%), Positives = 132/178 (74%)

Query: 1   MAAAVAELWETLKQAIVAYTGLSPXXXXXXXXXXXXLYHVVSGIFAGXXXXXXXXXXXXX 60
           MAAAVAELWETLKQAIVAYTGLSP            LYHVVSGIFAG             
Sbjct: 1   MAAAVAELWETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGPPPPPPPRPRDEP 60

Query: 61  XXXXXXXXVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAG 120
                   VQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAG
Sbjct: 61  EAEPLPPPVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAG 120

Query: 121 KDASRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMXXXXXXXXXXXXXPVEDG 178
           KDASRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFM             PVEDG
Sbjct: 121 KDASRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVGTVKKTVPVEDG 178



>Os02g0640300 Similar to Membrane steroid binding protein 1 (AtMP1)
          Length = 333

 Score =  177 bits (448), Expect = 7e-45,   Method: Compositional matrix adjust.
 Identities = 101/175 (57%), Positives = 108/175 (61%), Gaps = 3/175 (1%)

Query: 4   AVAELWETLKQAIVAYTGLSPXXXXXXXXXXXXLYHVVSGIFAGXXXXXXXXXXXXXXXX 63
           A AE WE     I AYTGLSP            LY  VSG+                   
Sbjct: 3   AAAEWWEAT---IAAYTGLSPAAFFTAVAVAAALYVAVSGLLTRRPPPLPRRQEEARASQ 59

Query: 64  XXXXXVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDA 123
                VQLGEV+EEELR YDGSDP KPLLMAIKGQIYDVTQSRMFYGPGGPYALFAG+DA
Sbjct: 60  PLPPPVQLGEVTEEELRVYDGSDPNKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGRDA 119

Query: 124 SRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMXXXXXXXXXXXXXPVEDG 178
           SRALAKMSFE  DLTGD+SGLGP EL+AL +WE KFM             PV +G
Sbjct: 120 SRALAKMSFELDDLTGDVSGLGPIELEALHEWEGKFMSKYVKVGTIKKIIPVSEG 174



>Os10g0502500 Cytochrome b5 domain containing protein
          Length = 265

 Score =  165 bits (417), Expect = 3e-41,   Method: Compositional matrix adjust.
 Identities = 78/91 (85%), Positives = 84/91 (92%)

Query: 69  VQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDASRALA 128
           VQ+GE++ EEL QYDGSDP+KPLLMAIKGQIYDV+QSR+FYGPGGPYALFAGKDASRALA
Sbjct: 116 VQVGEITAEELLQYDGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRALA 175

Query: 129 KMSFEPQDLTGDISGLGPFELDALQDWEYKF 159
           KMSFEPQDLT DISGL   EL ALQDWEYKF
Sbjct: 176 KMSFEPQDLTDDISGLSLLELSALQDWEYKF 206



>Os03g0321000 Similar to Steroid membrane binding protein-like
          Length = 191

 Score =  140 bits (352), Expect = 1e-33,   Method: Compositional matrix adjust.
 Identities = 74/162 (45%), Positives = 95/162 (58%), Gaps = 14/162 (8%)

Query: 12  LKQAIVAYTGLSPXXXXXXXXXXXXLYHVVSGIFAGXXXXXXXXXXXXXXXXXXXXX--- 68
           + +A+ AYTGLSP             Y +VS +F                          
Sbjct: 6   IAEAVQAYTGLSPAAAVTILALMLATYLLVSSLFVAPDAAPPKPPPQRKEEEQQQQQEEE 65

Query: 69  -----------VQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYAL 117
                      VQ+GE++ E+L  YDG DP KP+L+AI+GQ+YDVT+ R+FYGP GPY+L
Sbjct: 66  EAGAFVPYPDPVQVGEITLEQLAAYDGKDPAKPILIAIRGQVYDVTRGRLFYGPQGPYSL 125

Query: 118 FAGKDASRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKF 159
           FAG+DA+RALA MSF+P DLTGD+ GLGP EL+ LQDWE KF
Sbjct: 126 FAGRDATRALALMSFDPIDLTGDLDGLGPDELEVLQDWEDKF 167



>Os02g0793700 Cytochrome b5 domain containing protein
          Length = 102

 Score =  112 bits (279), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 50/87 (57%), Positives = 67/87 (77%)

Query: 73  EVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDASRALAKMSF 132
           E++  +LR YDGSDP KP+ ++++G++YDVT  R FYGPGG YA+FAG++ASRAL KMS 
Sbjct: 4   ELTAAQLRAYDGSDPSKPIYVSVRGKVYDVTSGRGFYGPGGAYAVFAGREASRALGKMSK 63

Query: 133 EPQDLTGDISGLGPFELDALQDWEYKF 159
           +  D++GD+SGL   EL  L DWE KF
Sbjct: 64  DDADVSGDLSGLSDKELGVLADWETKF 90



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,381,513
Number of extensions: 141900
Number of successful extensions: 190
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 188
Number of HSP's successfully gapped: 5
Length of query: 232
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 134
Effective length of database: 11,918,829
Effective search space: 1597123086
Effective search space used: 1597123086
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)