BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0498900 Os10g0498900|AK108732
         (332 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0498900  Similar to Microtubule-associated protein EB1-...   618   e-177
AK100467                                                          303   8e-83
Os04g0642100  Similar to Microtubule-associated protein EB1       226   1e-59

>Os10g0498900 Similar to Microtubule-associated protein EB1-like protein
          Length = 332

 Score =  618 bits (1593), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 299/332 (90%), Positives = 299/332 (90%)

Query: 1   MAASNIGMMDGAYFVGRNEILAWINTTLQLGLSKVEEAASGAVACQLMDAAHPGAVPMHK 60
           MAASNIGMMDGAYFVGRNEILAWINTTLQLGLSKVEEAASGAVACQLMDAAHPGAVPMHK
Sbjct: 1   MAASNIGMMDGAYFVGRNEILAWINTTLQLGLSKVEEAASGAVACQLMDAAHPGAVPMHK 60

Query: 61  VNFDAKTEYEMIQNYKVLQDVFNKLKITKHIEVNKLTKGRPLDNLEFMQWMKRYCDSVNG 120
           VNFDAKTEYEMIQNYKVLQDVFNKLKITKHIEVNKLTKGRPLDNLEFMQWMKRYCDSVNG
Sbjct: 61  VNFDAKTEYEMIQNYKVLQDVFNKLKITKHIEVNKLTKGRPLDNLEFMQWMKRYCDSVNG 120

Query: 121 GFMNSYNASERRESSKGGKETNRRTSVPSQAPAKSSSATHKAQASSHGAKKANGHXXXXX 180
           GFMNSYNASERRESSKGGKETNRRTSVPSQAPAKSSSATHKAQASSHGAKKANGH     
Sbjct: 121 GFMNSYNASERRESSKGGKETNRRTSVPSQAPAKSSSATHKAQASSHGAKKANGHAPNAP 180

Query: 181 XXXXXXXXXXXXXGPAYDEQITELKLLVDSLEKERDFYFSKLRDVEILCQSPEVEHLPIV 240
                        GPAYDEQITELKLLVDSLEKERDFYFSKLRDVEILCQSPEVEHLPIV
Sbjct: 181 PSRSAKPSPANSAGPAYDEQITELKLLVDSLEKERDFYFSKLRDVEILCQSPEVEHLPIV 240

Query: 241 NAIHKVLYAAEDDPSMVXXXXXXXXXXXXXXXPMLSPILEASEERPAKQEAHKRKSISDL 300
           NAIHKVLYAAEDDPSMV               PMLSPILEASEERPAKQEAHKRKSISDL
Sbjct: 241 NAIHKVLYAAEDDPSMVAEAQAMISQQQQSEQPMLSPILEASEERPAKQEAHKRKSISDL 300

Query: 301 ELEEFGMASSSRQRLSDISDVQLCGSPLTSFT 332
           ELEEFGMASSSRQRLSDISDVQLCGSPLTSFT
Sbjct: 301 ELEEFGMASSSRQRLSDISDVQLCGSPLTSFT 332



>AK100467 
          Length = 269

 Score =  303 bits (777), Expect = 8e-83,   Method: Compositional matrix adjust.
 Identities = 155/252 (61%), Positives = 179/252 (71%), Gaps = 14/252 (5%)

Query: 6   IGMMDGAYFVGRNEILAWINTTLQLGLSKVEEAASGAVACQLMDAAHPGAVPMHKVNFDA 65
           IGMMD AYFVGR EIL+WIN TLQL L+KVEEAASGAV CQLMD  HPG VPMHKVNFDA
Sbjct: 10  IGMMDSAYFVGRGEILSWINATLQLSLAKVEEAASGAVQCQLMDMVHPGVVPMHKVNFDA 69

Query: 66  KTEYEMIQNYKVLQDVFNKLKITKHIEVNKLTKGRPLDNLEFMQWMKRYCDSVNGGFMN- 124
           KTEY+MIQNYK+LQDVFNKL+++K+IEVNKL KGRPLDNLEF+QW+KRYCDSVNGG MN 
Sbjct: 70  KTEYDMIQNYKILQDVFNKLRLSKNIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGGIMNE 129

Query: 125 SYNASERRESSKGGKETNRRTSVPSQAPAKSSSATHKAQASSHGAKKANGHXXXXXXXXX 184
           +YN  ERR  SKG KE   R+   S   +KS  A   + A+S       G          
Sbjct: 130 NYNPVERR--SKGCKE---RSLKGSNKSSKSLQANRLSSANSADGGPCVGKVNVISEEHY 184

Query: 185 XXXXXXXXXGPAYDEQITELKLLVDSLEKERDFYFSKLRDVEILCQSPEVEHLPIVNAIH 244
                         E+I  LK+ +DS EKERDFYFSKLRD+EILCQ PE+EHLP+  A+ 
Sbjct: 185 IEQIQQ------LSEKIAGLKISMDSAEKERDFYFSKLRDIEILCQRPELEHLPMTKAVQ 238

Query: 245 KVLYAAE--DDP 254
           K+LYAA+  D P
Sbjct: 239 KILYAADARDSP 250



>Os04g0642100 Similar to Microtubule-associated protein EB1
          Length = 135

 Score =  226 bits (577), Expect = 1e-59,   Method: Compositional matrix adjust.
 Identities = 100/121 (82%), Positives = 110/121 (90%)

Query: 6   IGMMDGAYFVGRNEILAWINTTLQLGLSKVEEAASGAVACQLMDAAHPGAVPMHKVNFDA 65
           IGMMD AYFVGR EIL+WIN TLQL L+KVEEAASGAV CQLMD  HPG VPMHKVNFDA
Sbjct: 10  IGMMDSAYFVGRGEILSWINATLQLSLAKVEEAASGAVQCQLMDMVHPGVVPMHKVNFDA 69

Query: 66  KTEYEMIQNYKVLQDVFNKLKITKHIEVNKLTKGRPLDNLEFMQWMKRYCDSVNGGFMNS 125
           KTEY+MIQNYK+LQDVFNKL+++K+IEVNKL KGRPLDNLEF+QW+KRYCDSVNGG MN 
Sbjct: 70  KTEYDMIQNYKILQDVFNKLRLSKNIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGGIMNE 129

Query: 126 Y 126
           Y
Sbjct: 130 Y 130



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.128    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,583,051
Number of extensions: 346709
Number of successful extensions: 1042
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1040
Number of HSP's successfully gapped: 3
Length of query: 332
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 231
Effective length of database: 11,762,187
Effective search space: 2717065197
Effective search space used: 2717065197
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 156 (64.7 bits)