BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0446800 Os10g0446800|J100070K17
         (90 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0446800  Similar to Ketol-acid reductoisomerase, chloro...   183   2e-47
Os05g0573700  Similar to Ketol-acid reductoisomerase, chloro...    89   6e-19
Os01g0652600  Similar to Ketol-acid reductoisomerase, chloro...    64   2e-11

>Os10g0446800 Similar to Ketol-acid reductoisomerase, chloroplast precursor (EC
          1.1.1.86) (Acetohydroxy-acid reductoisomerase)
          (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 90

 Score =  183 bits (464), Expect = 2e-47,   Method: Compositional matrix adjust.
 Identities = 90/90 (100%), Positives = 90/90 (100%)

Query: 1  KITRAIQFVDLDIIIHKPLYFVLLTYAILHCSVSPTFYYALSFHIGLRKGSKSFDEACAA 60
          KITRAIQFVDLDIIIHKPLYFVLLTYAILHCSVSPTFYYALSFHIGLRKGSKSFDEACAA
Sbjct: 1  KITRAIQFVDLDIIIHKPLYFVLLTYAILHCSVSPTFYYALSFHIGLRKGSKSFDEACAA 60

Query: 61 GFTEESGTLGDIWETVSGSDLMLLLISDTA 90
          GFTEESGTLGDIWETVSGSDLMLLLISDTA
Sbjct: 61 GFTEESGTLGDIWETVSGSDLMLLLISDTA 90



>Os05g0573700 Similar to Ketol-acid reductoisomerase, chloroplast precursor (EC
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 578

 Score = 89.4 bits (220), Expect = 6e-19,   Method: Composition-based stats.
 Identities = 43/46 (93%), Positives = 44/46 (95%)

Query: 45  IGLRKGSKSFDEACAAGFTEESGTLGDIWETVSGSDLMLLLISDTA 90
           IGLRKGSKSFDEA AAGFTEESGTLGDIWETVSGSDL+LLLISD A
Sbjct: 141 IGLRKGSKSFDEARAAGFTEESGTLGDIWETVSGSDLVLLLISDAA 186



>Os01g0652600 Similar to Ketol-acid reductoisomerase, chloroplast precursor (EC
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
          Length = 548

 Score = 63.9 bits (154), Expect = 2e-11,   Method: Composition-based stats.
 Identities = 29/31 (93%), Positives = 30/31 (96%)

Query: 45  IGLRKGSKSFDEACAAGFTEESGTLGDIWET 75
           IGLRKGSKSF+EA AAGFTEESGTLGDIWET
Sbjct: 111 IGLRKGSKSFEEARAAGFTEESGTLGDIWET 141



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.140    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,837,780
Number of extensions: 98781
Number of successful extensions: 220
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 220
Number of HSP's successfully gapped: 3
Length of query: 90
Length of database: 17,035,801
Length adjustment: 60
Effective length of query: 30
Effective length of database: 13,902,961
Effective search space: 417088830
Effective search space used: 417088830
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)