BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0405300 Os10g0405300|AK066928
         (118 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0405300  Conserved hypothetical protein                      200   2e-52
Os03g0169900  Conserved hypothetical protein                      132   7e-32

>Os10g0405300 Conserved hypothetical protein
          Length = 118

 Score =  200 bits (509), Expect = 2e-52,   Method: Compositional matrix adjust.
 Identities = 98/118 (83%), Positives = 98/118 (83%)

Query: 1   MESRKEEEQHGGGGAVGWMTVPAFGEWDMKNGAVPDYSMDFSKIREMRKQNKRELSRASL 60
           MESRKEEEQHGGGGAVGWMTVPAFGEWDMKNGAVPDYSMDFSKIREMRKQNKRELSRASL
Sbjct: 1   MESRKEEEQHGGGGAVGWMTVPAFGEWDMKNGAVPDYSMDFSKIREMRKQNKRELSRASL 60

Query: 61  GGDDDLLXXXXXXXXXXXXXXXXXXXXDDHRRPLHAAHDDSPTGRKKFLSYFQCCIRA 118
           GGDDDLL                    DDHRRPLHAAHDDSPTGRKKFLSYFQCCIRA
Sbjct: 61  GGDDDLLAAQQHKAAAPQPAPNASAAADDHRRPLHAAHDDSPTGRKKFLSYFQCCIRA 118



>Os03g0169900 Conserved hypothetical protein
          Length = 116

 Score =  132 bits (331), Expect = 7e-32,   Method: Compositional matrix adjust.
 Identities = 70/106 (66%), Positives = 75/106 (70%), Gaps = 5/106 (4%)

Query: 17  GWMTVPAFGEWDMKNGAVPDYSMDFSKIREMRKQNKRELSRASLGGDDDLLXXXXXXXXX 76
           GWMTVPAFG+WDMKNGA+PDYSMDFSKIREMRKQNK+ELSR SLGGDDDLL         
Sbjct: 12  GWMTVPAFGDWDMKNGALPDYSMDFSKIREMRKQNKKELSRTSLGGDDDLLAQQKQQQPQ 71

Query: 77  XXXXXXXXXX---XDDHR-RPLHAAHDDSPTGRKKFLSYFQCCIRA 118
                         DDHR R LH  H  SPTGR+ FLSYFQCCI+A
Sbjct: 72  PQQKPAKANLGRPADDHRHRQLHGRH-GSPTGRRTFLSYFQCCIKA 116



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,391,382
Number of extensions: 147515
Number of successful extensions: 687
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 686
Number of HSP's successfully gapped: 2
Length of query: 118
Length of database: 17,035,801
Length adjustment: 85
Effective length of query: 33
Effective length of database: 12,597,611
Effective search space: 415721163
Effective search space used: 415721163
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)