BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0337600 Os10g0337600|J065013H19
         (89 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0337600  Got1-like protein family protein                    144   1e-35
Os03g0209400  Got1-like protein family protein                     94   2e-20

>Os10g0337600 Got1-like protein family protein
          Length = 89

 Score =  144 bits (363), Expect = 1e-35,   Method: Compositional matrix adjust.
 Identities = 74/89 (83%), Positives = 74/89 (83%)

Query: 1  MVSFEMNDLQKIGIGLTXXXXXXXXXXXXXXXDKGLLAMGNILFLSGLGLTIGLKSTLQF 60
          MVSFEMNDLQKIGIGLT               DKGLLAMGNILFLSGLGLTIGLKSTLQF
Sbjct: 1  MVSFEMNDLQKIGIGLTGFGVFFSFLGIIFFFDKGLLAMGNILFLSGLGLTIGLKSTLQF 60

Query: 61 FTKPKNYKVCKFTVAYQSFCPWTICYLIN 89
          FTKPKNYKVCKFTVAYQSFCPWTICYLIN
Sbjct: 61 FTKPKNYKVCKFTVAYQSFCPWTICYLIN 89



>Os03g0209400 Got1-like protein family protein
          Length = 140

 Score = 94.4 bits (233), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 49/68 (72%), Positives = 53/68 (77%)

Query: 1  MVSFEMNDLQKIGIGLTXXXXXXXXXXXXXXXDKGLLAMGNILFLSGLGLTIGLKSTLQF 60
          MVSFEMNDL+KIG+GLT               DKGL+AMGNILFLSGLGLTIGLKST+QF
Sbjct: 1  MVSFEMNDLKKIGLGLTGFGVFFSFLGIIFFFDKGLIAMGNILFLSGLGLTIGLKSTMQF 60

Query: 61 FTKPKNYK 68
          FTKPKNYK
Sbjct: 61 FTKPKNYK 68



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.144    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,046,003
Number of extensions: 51672
Number of successful extensions: 90
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 88
Number of HSP's successfully gapped: 2
Length of query: 89
Length of database: 17,035,801
Length adjustment: 59
Effective length of query: 30
Effective length of database: 13,955,175
Effective search space: 418655250
Effective search space used: 418655250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 149 (62.0 bits)