BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0541900 Os09g0541900|AK101536
         (487 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0541900  Similar to 26S proteasome subunit RPN3a             826   0.0  
Os08g0550100  Similar to 26S proteasome subunit RPN3a             761   0.0  
AK108887                                                          296   3e-80

>Os09g0541900 Similar to 26S proteasome subunit RPN3a
          Length = 487

 Score =  826 bits (2133), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 413/487 (84%), Positives = 413/487 (84%)

Query: 1   MPEDVEMNDSXXXXXXXXXXXXSTLQHLKEIASVIEAGSLTKEVRRISXXXXXXXXXXXX 60
           MPEDVEMNDS            STLQHLKEIASVIEAGSLTKEVRRIS            
Sbjct: 1   MPEDVEMNDSQPQPAAAPPPAASTLQHLKEIASVIEAGSLTKEVRRISRAVRLTVALRRR 60

Query: 61  XXXXXXXXXXXXXXPHSSEAFARLSSLLPKEDGSEMDVDLAAPAAQVSIKHGLPEIEIYC 120
                         PHSSEAFARLSSLLPKEDGSEMDVDLAAPAAQVSIKHGLPEIEIYC
Sbjct: 61  LAARDVAAFLAFALPHSSEAFARLSSLLPKEDGSEMDVDLAAPAAQVSIKHGLPEIEIYC 120

Query: 121 YLLVLIFLIDNKKYDEAKACASASITRLKNLNRRTVDVLASRVFTYYSYVHELTSSLAEI 180
           YLLVLIFLIDNKKYDEAKACASASITRLKNLNRRTVDVLASRVFTYYSYVHELTSSLAEI
Sbjct: 121 YLLVLIFLIDNKKYDEAKACASASITRLKNLNRRTVDVLASRVFTYYSYVHELTSSLAEI 180

Query: 181 RGTLLALHRMATLHRDELGQETXXXXXXXXXXXXXXXDQAEKLRSKAPRFEAHSNQQFCR 240
           RGTLLALHRMATLHRDELGQET               DQAEKLRSKAPRFEAHSNQQFCR
Sbjct: 181 RGTLLALHRMATLHRDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQFCR 240

Query: 241 YLFYLGKIRTIQLEYTDAKESLLQAARKAPTTARGFRVQCNKWAIIVRLLLGEIPERTVF 300
           YLFYLGKIRTIQLEYTDAKESLLQAARKAPTTARGFRVQCNKWAIIVRLLLGEIPERTVF
Sbjct: 241 YLFYLGKIRTIQLEYTDAKESLLQAARKAPTTARGFRVQCNKWAIIVRLLLGEIPERTVF 300

Query: 301 MQKGMKAALAPYFELTNAVRVGDLELFRAVAEKFASTFSADRTRNLIVRLRHNVIRTGLR 360
           MQKGMKAALAPYFELTNAVRVGDLELFRAVAEKFASTFSADRTRNLIVRLRHNVIRTGLR
Sbjct: 301 MQKGMKAALAPYFELTNAVRVGDLELFRAVAEKFASTFSADRTRNLIVRLRHNVIRTGLR 360

Query: 361 NISISYSRISLADIAKKLRLDSENPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGD 420
           NISISYSRISLADIAKKLRLDSENPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGD
Sbjct: 361 NISISYSRISLADIAKKLRLDSENPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGD 420

Query: 421 VYSTNEPQIAFNSRIAFCLNMHNEAVKALRFPPNSHXXXXXXXXXXXXXXXXXXXXXHMA 480
           VYSTNEPQIAFNSRIAFCLNMHNEAVKALRFPPNSH                     HMA
Sbjct: 421 VYSTNEPQIAFNSRIAFCLNMHNEAVKALRFPPNSHKEKESAEKRRERLQQEEELAKHMA 480

Query: 481 EEDDDDF 487
           EEDDDDF
Sbjct: 481 EEDDDDF 487



>Os08g0550100 Similar to 26S proteasome subunit RPN3a
          Length = 486

 Score =  761 bits (1966), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 376/484 (77%), Positives = 396/484 (81%)

Query: 4   DVEMNDSXXXXXXXXXXXXSTLQHLKEIASVIEAGSLTKEVRRISXXXXXXXXXXXXXXX 63
           DV+M+DS            S L HLKEIASVIEAGSL+KEVRRIS               
Sbjct: 3   DVQMSDSEPQAAPPAAPAASALHHLKEIASVIEAGSLSKEVRRISRAVRLTVALRRRLAA 62

Query: 64  XXXXXXXXXXXPHSSEAFARLSSLLPKEDGSEMDVDLAAPAAQVSIKHGLPEIEIYCYLL 123
                      P SSEA+ RL+SL+PKED +EMD+D+AAPA Q+SIKHGLPEIEIYCYLL
Sbjct: 63  RDVSAFLAFALPPSSEAYTRLTSLVPKEDDTEMDIDMAAPATQISIKHGLPEIEIYCYLL 122

Query: 124 VLIFLIDNKKYDEAKACASASITRLKNLNRRTVDVLASRVFTYYSYVHELTSSLAEIRGT 183
           VLIFLID KKYD+AKACA+ASI RLKNLNRRTVDVLASR++ YYSYVHELT+SLAEIRG 
Sbjct: 123 VLIFLIDQKKYDDAKACANASIARLKNLNRRTVDVLASRLYFYYSYVHELTNSLAEIRGN 182

Query: 184 LLALHRMATLHRDELGQETXXXXXXXXXXXXXXXDQAEKLRSKAPRFEAHSNQQFCRYLF 243
           LLALHRMATLHRDELGQET               DQAEKLRSKAPRFEAHSNQQFCRYLF
Sbjct: 183 LLALHRMATLHRDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQFCRYLF 242

Query: 244 YLGKIRTIQLEYTDAKESLLQAARKAPTTARGFRVQCNKWAIIVRLLLGEIPERTVFMQK 303
           YLGKIRTIQLEYTDAKESLLQAARKAP TARGFR+QCNKWAIIVRLLLGEIPERTVFMQK
Sbjct: 243 YLGKIRTIQLEYTDAKESLLQAARKAPITARGFRIQCNKWAIIVRLLLGEIPERTVFMQK 302

Query: 304 GMKAALAPYFELTNAVRVGDLELFRAVAEKFASTFSADRTRNLIVRLRHNVIRTGLRNIS 363
           GMK AL PYFELTNAVR+GDLELFRAVA+KF STFSADRTRNLIVRLRHNVIRTGLRNIS
Sbjct: 303 GMKEALTPYFELTNAVRIGDLELFRAVADKFVSTFSADRTRNLIVRLRHNVIRTGLRNIS 362

Query: 364 ISYSRISLADIAKKLRLDSENPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYS 423
           ISYSRISLADIAKKLRLDS+NPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYS
Sbjct: 363 ISYSRISLADIAKKLRLDSDNPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYS 422

Query: 424 TNEPQIAFNSRIAFCLNMHNEAVKALRFPPNSHXXXXXXXXXXXXXXXXXXXXXHMAEED 483
           TNEPQIAFNSRIAFCLNMHNEAVKA+RFPPNSH                     HMAEED
Sbjct: 423 TNEPQIAFNSRIAFCLNMHNEAVKAMRFPPNSHKEKESAEKRRERLQQEEELAKHMAEED 482

Query: 484 DDDF 487
           DDDF
Sbjct: 483 DDDF 486



>AK108887 
          Length = 648

 Score =  296 bits (757), Expect = 3e-80,   Method: Compositional matrix adjust.
 Identities = 167/370 (45%), Positives = 212/370 (57%), Gaps = 27/370 (7%)

Query: 113 LPEIEIYCYLLVLIFLIDNKKYDEAKACASASITRLKNLNRRTVDVLASRVFTYYSYVHE 172
           LPEI+ Y +LLV+I+LID+   +EA A A+A    L   NRR++D L+++   YY   HE
Sbjct: 253 LPEIQTYLHLLVIIYLIDHSAKEEAVAAATALNDFLAKHNRRSLDALSAKALYYYVLTHE 312

Query: 173 LTSSLAEIRGTLLALHRMATLHRDELGQETXXXXXXXXXXXXXXXDQAEKLRSKAPRFEA 232
                  IR  LL  HR A L  DE GQ                  QA+K R  +  F  
Sbjct: 313 KVGRANAIRNNLLLAHRTACLRHDEPGQAVLTNAILRNYLQNNLYTQADKFRLNS-HFPT 371

Query: 233 H-SNQQFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTT-ARGFRVQCNKWAIIVRLL 290
           H SN Q  RYL+Y+GKI+T+QL Y+DA  SL Q+ RKAPT  A GFR    K  +IV+LL
Sbjct: 372 HISNSQHARYLYYIGKIQTVQLHYSDAYTSLSQSIRKAPTAGAIGFRQTVTKLLVIVQLL 431

Query: 291 LGEIPERTVFMQKGMKAALAPYFELTNAVRVGDLELFRAVAEKFASTFSADRTRNLIVRL 350
           +G+IPER++F    +K +L PYF+LT AVRVG+L  F        + F  D     I RL
Sbjct: 432 MGDIPERSLFRTPQLKRSLKPYFQLTQAVRVGNLAAFGTCVASHEAIFRRDHMLTFIARL 491

Query: 351 RHNVIRTGLRNISISYSRISLADIAKKLRLDSENPVA----------------------- 387
           RHNVI+TGLR I+ISYSRIS+ DI  KL L      A                       
Sbjct: 492 RHNVIKTGLRRINISYSRISIHDICDKLHLTEATAHAGSPHHAASVGAAAAAGISVGSSE 551

Query: 388 -DAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYSTNEPQIAFNSRIAFCLNMHNEAV 446
            DAE IVAKAI DG I+ATID    ++ SK+  DVY+TNEPQ  F+ RI FCL+ +N AV
Sbjct: 552 EDAEFIVAKAIHDGVIEATIDRKQHFVFSKDNADVYATNEPQQQFHKRIQFCLDSYNAAV 611

Query: 447 KALRFPPNSH 456
           K +RFPP++H
Sbjct: 612 KDMRFPPDAH 621



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.132    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,841,178
Number of extensions: 382481
Number of successful extensions: 882
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 876
Number of HSP's successfully gapped: 3
Length of query: 487
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 382
Effective length of database: 11,553,331
Effective search space: 4413372442
Effective search space used: 4413372442
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)