BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0538500 Os09g0538500|AK121538
         (515 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0538500  Similar to Male sterility MS5                       879   0.0  
Os05g0506000  Similar to MS5-like protein (Fragment)              296   2e-80
Os08g0130300  Tetratricopeptide-like helical domain containi...   249   2e-66
Os03g0165900  Tetratricopeptide-like helical domain containi...   239   3e-63

>Os09g0538500 Similar to Male sterility MS5
          Length = 515

 Score =  879 bits (2271), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 448/515 (86%), Positives = 448/515 (86%)

Query: 1   MMXXXXXXETWLAAAAAVGLRPTKSAPCSPIKPXXXXXXXXXXXXMLRTNSDSFHVAHKV 60
           MM      ETWLAAAAAVGLRPTKSAPCSPIKP            MLRTNSDSFHVAHKV
Sbjct: 1   MMQQQQQQETWLAAAAAVGLRPTKSAPCSPIKPAESSASAAAAAAMLRTNSDSFHVAHKV 60

Query: 61  PVGDTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQQNRAEEAIEA 120
           PVGDTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQQNRAEEAIEA
Sbjct: 61  PVGDTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQQNRAEEAIEA 120

Query: 121 IKSLRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAFNGKRTKTARS 180
           IKSLRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAFNGKRTKTARS
Sbjct: 121 IKSLRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAFNGKRTKTARS 180

Query: 181 QGRKFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKMCNLGICLMKQ 240
           QGRKFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKMCNLGICLMKQ
Sbjct: 181 QGRKFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKMCNLGICLMKQ 240

Query: 241 GRVLEAKDVLKQVRPAGVDGLRGADSHLKAYERAQEMLRDLEAKLVGRRLPRAGDQLVDK 300
           GRVLEAKDVLKQVRPAGVDGLRGADSHLKAYERAQEMLRDLEAKLVGRRLPRAGDQLVDK
Sbjct: 241 GRVLEAKDVLKQVRPAGVDGLRGADSHLKAYERAQEMLRDLEAKLVGRRLPRAGDQLVDK 300

Query: 301 SWLFDALLLGSSSSIWQPQPCIDHMLXXXXXXXRDQFADEXXXXXXXXXXXXXXXLQPNI 360
           SWLFDALLLGSSSSIWQPQPCIDHML       RDQFADE               LQPNI
Sbjct: 301 SWLFDALLLGSSSSIWQPQPCIDHMLPPPPPPPRDQFADENAAAAAANKKAAAAALQPNI 360

Query: 361 LRVDAQPFYSLRMPPLATKPQNIQQKPPTPQPQVHDPMGNLKRTRSGNAMDKXXXXXXGP 420
           LRVDAQPFYSLRMPPLATKPQNIQQKPPTPQPQVHDPMGNLKRTRSGNAMDK      GP
Sbjct: 361 LRVDAQPFYSLRMPPLATKPQNIQQKPPTPQPQVHDPMGNLKRTRSGNAMDKAAAAAAGP 420

Query: 421 VEKEPINDENSGRRKSLSAEERWPELPDHSAFXXXXXXXXXXXXXXXXXXXXXERNDNCC 480
           VEKEPINDENSGRRKSLSAEERWPELPDHSAF                     ERNDNCC
Sbjct: 421 VEKEPINDENSGRRKSLSAEERWPELPDHSAFDEALVAAVLAPVLDDSAAAAAERNDNCC 480

Query: 481 KPAPPAGCDTSPAMKEKIGKRLRIFQDITQTLNTF 515
           KPAPPAGCDTSPAMKEKIGKRLRIFQDITQTLNTF
Sbjct: 481 KPAPPAGCDTSPAMKEKIGKRLRIFQDITQTLNTF 515



>Os05g0506000 Similar to MS5-like protein (Fragment)
          Length = 299

 Score =  296 bits (759), Expect = 2e-80,   Method: Compositional matrix adjust.
 Identities = 144/231 (62%), Positives = 179/231 (77%), Gaps = 4/231 (1%)

Query: 52  DSFHVAHKVPVGDTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQQ 111
           D FHV HKVP GD+PYVRAK +QLVDKDPE AI  FW AIN+ D+VDSALKDMA+VMKQQ
Sbjct: 20  DLFHVVHKVPAGDSPYVRAKHLQLVDKDPETAIVWFWKAINSRDKVDSALKDMAVVMKQQ 79

Query: 112 NRAEEAIEAIKSLRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAFN 171
           +RA+EAIEAI+S R  CS QAQESLDN+L+DLYK+CG++D+QI LLK KL++I+ G AFN
Sbjct: 80  DRAKEAIEAIRSFRHLCSRQAQESLDNLLIDLYKKCGKVDEQIDLLKQKLKMIYLGEAFN 139

Query: 172 GKRTKTARSQGRKFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKMC 231
           GK TKTARS G+KFQV+++QE +R+LGNLGWA MQ+ NY+ AE  YR+A  I PD N+ C
Sbjct: 140 GKATKTARSHGKKFQVSIQQETSRILGNLGWAYMQQSNYSAAELVYRKAQSIEPDANRAC 199

Query: 232 NLGICLMKQGRVLEAKDVLKQVRPAGVDGLRGADSHLKAYERAQEMLRDLE 282
           NLG+CL+KQ R  EA+ VL  V    + G    D   K   RA+++L +LE
Sbjct: 200 NLGLCLIKQSRHDEARQVLHDVVLRRISG--SEDD--KVVARAKQLLHELE 246



>Os08g0130300 Tetratricopeptide-like helical domain containing protein
          Length = 769

 Score =  249 bits (637), Expect = 2e-66,   Method: Compositional matrix adjust.
 Identities = 124/220 (56%), Positives = 162/220 (73%), Gaps = 5/220 (2%)

Query: 64  DTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQQNRAEEAIEAIKS 123
           D+PYVRAK+ Q+++KDP KA+ LFWAAIN+GDR++SALKDMA V+KQ NRAEEAIEAI+S
Sbjct: 85  DSPYVRAKQAQVIEKDPNKAVPLFWAAINSGDRIESALKDMATVLKQANRAEEAIEAIRS 144

Query: 124 LRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAFNGKRTKTARSQGR 183
            R RC ++AQESLDNILLDLYK+CGR  +QI +L  KL+++ +  A    +TK ++S GR
Sbjct: 145 FRDRCPNEAQESLDNILLDLYKKCGRTKEQIEMLTLKLRIVDEELASGRWKTKLSKSHGR 204

Query: 184 KFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKMCNLGICLMKQGRV 243
              ++L  E  RLLGNL WA MQ ENY EAE  YR+AL I  D NK CNL ICL+K G+V
Sbjct: 205 VVYLSLRDEKARLLGNLAWAHMQSENYDEAEMLYRQALAIEADYNKECNLAICLIKTGKV 264

Query: 244 LEAKDVLKQVRPAGVDGLRGADSHLKAYERAQEMLRDLEA 283
            EAK +L+ +     D     +SH+++  RA+EML +LE+
Sbjct: 265 AEAKYLLQSIPDNCSD-----ESHVRSLARAREMLMELES 299



>Os03g0165900 Tetratricopeptide-like helical domain containing protein
          Length = 315

 Score =  239 bits (611), Expect = 3e-63,   Method: Compositional matrix adjust.
 Identities = 117/203 (57%), Positives = 151/203 (74%)

Query: 51  SDSFHVAHKVPVGDTPYVRAKRVQLVDKDPEKAIALFWAAINAGDRVDSALKDMAIVMKQ 110
           S + HVA+K+P GD PY RAK  QLV+KD + +IA FW AIN+GD+VDSALKDMA+VMKQ
Sbjct: 15  SAAAHVAYKIPAGDGPYARAKHYQLVEKDLDASIAWFWKAINSGDKVDSALKDMAVVMKQ 74

Query: 111 QNRAEEAIEAIKSLRSRCSDQAQESLDNILLDLYKRCGRLDDQISLLKHKLQLIHQGHAF 170
           +    +AI+AIKSLR  C  Q+Q+SLDNILLDLYK  GR  ++I LLK KL+ I+ G AF
Sbjct: 75  RGYHADAIDAIKSLRHLCPKQSQDSLDNILLDLYKASGRTKEEIELLKQKLRKIYLGEAF 134

Query: 171 NGKRTKTARSQGRKFQVTLEQEATRLLGNLGWALMQKENYTEAEGAYRRALLIGPDNNKM 230
           +GK TK ARS GRK  V+++QE +R+LGNL WA MQ+ N+  AE  YR+A ++ PD NK 
Sbjct: 135 HGKTTKRARSHGRKIHVSVKQETSRVLGNLAWAYMQQGNFMAAEVVYRKAQMVDPDANKA 194

Query: 231 CNLGICLMKQGRVLEAKDVLKQV 253
           CNL +CL++Q R  +A+ VL  V
Sbjct: 195 CNLALCLIEQRRPADAEAVLAGV 217



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.133    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,899,370
Number of extensions: 614676
Number of successful extensions: 2707
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 2705
Number of HSP's successfully gapped: 4
Length of query: 515
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 410
Effective length of database: 11,553,331
Effective search space: 4736865710
Effective search space used: 4736865710
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)