BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0516600 Os09g0516600|AY054407
         (336 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0516600  Glyoxalase II                                       675   0.0  
Os03g0332400  Similar to Hydroxyacylglutathione hydrolase cy...   173   2e-43
Os01g0667200  Similar to Glyoxalase II                             67   2e-11

>Os09g0516600 Glyoxalase II
          Length = 336

 Score =  675 bits (1742), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 325/336 (96%), Positives = 325/336 (96%)

Query: 1   MRMLSKACSLVASSLPRCSSSAAPTIRGQPSLLPSVRKEWLGKPLLYGIGTLLVMPLRTL 60
           MRMLSKACSLVASSLPRCSSSAAPTIRGQPSLLPSVRKEWLGKPLLYGIGTLLVMPLRTL
Sbjct: 1   MRMLSKACSLVASSLPRCSSSAAPTIRGQPSLLPSVRKEWLGKPLLYGIGTLLVMPLRTL 60

Query: 61  HGVGRMFGAGRFLCNMTSVSSSLQIELVPCLQDNYAYILHXXXXXXXXXXXPSEATPIIN 120
           HGVGRMFGAGRFLCNMTSVSSSLQIELVPCLQDNYAYILH           PSEATPIIN
Sbjct: 61  HGVGRMFGAGRFLCNMTSVSSSLQIELVPCLQDNYAYILHDVDTGTVGVVDPSEATPIIN 120

Query: 121 ALEKRNQNLTYILNTHHHYDHTGGNLELKAKYGAKVIGSAKDRDRIPGIDITLSEGDTWM 180
           ALEKRNQNLTYILNTHHHYDHTGGNLELKAKYGAKVIGSAKDRDRIPGIDITLSEGDTWM
Sbjct: 121 ALEKRNQNLTYILNTHHHYDHTGGNLELKAKYGAKVIGSAKDRDRIPGIDITLSEGDTWM 180

Query: 181 FAGHQVLVMETPGHTSGHVCYHFPGSGAIFTGDTLFSLSCGKLFEGTPQQMYSSLQKIIA 240
           FAGHQVLVMETPGHTSGHVCYHFPGSGAIFTGDTLFSLSCGKLFEGTPQQMYSSLQKIIA
Sbjct: 181 FAGHQVLVMETPGHTSGHVCYHFPGSGAIFTGDTLFSLSCGKLFEGTPQQMYSSLQKIIA 240

Query: 241 LPDETRVYCGHEYTLSNSKFALSIEPGNKDLQEYAANAADLRKRNTPTVPTTIGREKQCN 300
           LPDETRVYCGHEYTLSNSKFALSIEPGNKDLQEYAANAADLRKRNTPTVPTTIGREKQCN
Sbjct: 241 LPDETRVYCGHEYTLSNSKFALSIEPGNKDLQEYAANAADLRKRNTPTVPTTIGREKQCN 300

Query: 301 PFLRTSSPEIKNTLSIPDHFDDARVLEVVRRAKDNF 336
           PFLRTSSPEIKNTLSIPDHFDDARVLEVVRRAKDNF
Sbjct: 301 PFLRTSSPEIKNTLSIPDHFDDARVLEVVRRAKDNF 336



>Os03g0332400 Similar to Hydroxyacylglutathione hydrolase cytoplasmic (EC
           3.1.2.6) (Glyoxalase II) (Glx II)
          Length = 258

 Score =  173 bits (438), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 96/261 (36%), Positives = 139/261 (53%), Gaps = 12/261 (4%)

Query: 83  LQIELVPCLQDNYAYILHXXXXXXXXXXXPSEATPIINALEKRNQNLTYILNTHHHYDHT 142
           ++I  V CL+DNYAY++            P E   ++ A  +    +  +L THHH+DH 
Sbjct: 1   MKIIPVACLEDNYAYLIVDESTKSAAAVDPVEPEKVLAAAAEVGVRIDCVLTTHHHWDHA 60

Query: 143 GGNLELKAKY-GAKVIGSAKDRDRIPGIDITLSEGDTWMFAGH-QVLVMETPGHTSGHVC 200
           GGN ++     G KV G +   D + G    +  G         ++L + TP HT GH+ 
Sbjct: 61  GGNEKMAQSVPGIKVYGGS--LDNVKGCTDQVENGTKLSLGKDIEILCLHTPCHTKGHIS 118

Query: 201 YHFPGSG----AIFTGDTLFSLSCGKLFEGTPQQMYSSLQKII-ALPDETRVYCGHEYTL 255
           Y+         A+FTGDTLF   CG+ FEGT +QMY SL   + +LP  T+VYCGHEYT+
Sbjct: 119 YYVTSKEEEDPAVFTGDTLFIAGCGRFFEGTAEQMYQSLCVTLGSLPKPTQVYCGHEYTV 178

Query: 256 SNSKFALSIEPGNKDLQEYAANAADLRKRNTPTVPTTIGREKQCNPFLRTSSPEIKNTLS 315
            N KF L++EP N+ +++    A   R+ N PT+P+TIG E + N F+R   PEI+    
Sbjct: 179 KNLKFILTVEPDNEKVKQKLEWAQKQREANQPTIPSTIGEEFETNTFMRVDLPEIQAKFG 238

Query: 316 IPDHFDDARVLEVVRRAKDNF 336
                +    L  VR+ KDN+
Sbjct: 239 AKSPVE---ALREVRKTKDNW 256



>Os01g0667200 Similar to Glyoxalase II
          Length = 305

 Score = 66.6 bits (161), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 47/145 (32%), Positives = 71/145 (48%), Gaps = 13/145 (8%)

Query: 119 INALEKRNQNLTYILNTHHHYDHTGGNLELKAKY-GAKVIGSAKDRDRIPGIDITLSEGD 177
           +N +++    L Y +NTH H DH  G   +K K  G K + +   + +    D  +  GD
Sbjct: 104 LNLIKELGLKLVYAMNTHVHADHVTGTGLIKTKLPGVKSVIAKVSKAK---ADHFIEHGD 160

Query: 178 TWMFAGHQVLVMETPGHTSGHVCY------HFPGSGAIFTGDTLFSLSCGKL-FE-GTPQ 229
              F    + V  TPGHT+G V Y        P     FTGD L   +CG+  F+ G+  
Sbjct: 161 KIYFGNLFLEVRSTPGHTAGCVTYVTGEGDDQPSPRMAFTGDALLIRACGRTDFQGGSSD 220

Query: 230 QMYSSLQ-KIIALPDETRVYCGHEY 253
           ++Y S+  +I  LP +T +Y GH+Y
Sbjct: 221 ELYESVHSQIFTLPKDTLLYPGHDY 245



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,589,706
Number of extensions: 484720
Number of successful extensions: 919
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 914
Number of HSP's successfully gapped: 3
Length of query: 336
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 235
Effective length of database: 11,762,187
Effective search space: 2764113945
Effective search space used: 2764113945
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)