BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0516500 Os09g0516500|012-091-H12
         (187 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0516500  Reticulon family protein                            374   e-104
Os03g0405000  Reticulon family protein                             88   3e-18

>Os09g0516500 Reticulon family protein
          Length = 187

 Score =  374 bits (960), Expect = e-104,   Method: Compositional matrix adjust.
 Identities = 187/187 (100%), Positives = 187/187 (100%)

Query: 1   NFVAAADILLWRDEKKTFSYVTVLFLLFYWFLLSDRTFVSSAAKILLVISLALFIHGVLP 60
           NFVAAADILLWRDEKKTFSYVTVLFLLFYWFLLSDRTFVSSAAKILLVISLALFIHGVLP
Sbjct: 1   NFVAAADILLWRDEKKTFSYVTVLFLLFYWFLLSDRTFVSSAAKILLVISLALFIHGVLP 60

Query: 61  PQVFGFTVEKVTSDYFEVSQETLKNTLVWMASIWNGGIYKLRVLAEGDDWTTFLKAFAFL 120
           PQVFGFTVEKVTSDYFEVSQETLKNTLVWMASIWNGGIYKLRVLAEGDDWTTFLKAFAFL
Sbjct: 61  PQVFGFTVEKVTSDYFEVSQETLKNTLVWMASIWNGGIYKLRVLAEGDDWTTFLKAFAFL 120

Query: 121 YCVKVMLNLQFRMLMGLVLAFMFVVFIVYEQCEEEIDSLVAFASVKVKSLVGKVIGNLPD 180
           YCVKVMLNLQFRMLMGLVLAFMFVVFIVYEQCEEEIDSLVAFASVKVKSLVGKVIGNLPD
Sbjct: 121 YCVKVMLNLQFRMLMGLVLAFMFVVFIVYEQCEEEIDSLVAFASVKVKSLVGKVIGNLPD 180

Query: 181 ALKAYIS 187
           ALKAYIS
Sbjct: 181 ALKAYIS 187



>Os03g0405000 Reticulon family protein
          Length = 561

 Score = 88.2 bits (217), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 50/158 (31%), Positives = 85/158 (53%)

Query: 6   ADILLWRDEKKTFSYVTVLFLLFYWFLLSDRTFVSSAAKILLVISLALFIHGVLPPQVFG 65
           A  +LW D K+T + + +L +++Y       TF+++ AK+  + +L LFIHG+LP  VFG
Sbjct: 383 AKTVLWEDMKQTMTVLLLLAVIYYHLFTCGYTFITAMAKLFSLTALFLFIHGILPANVFG 442

Query: 66  FTVEKVTSDYFEVSQETLKNTLVWMASIWNGGIYKLRVLAEGDDWTTFLKAFAFLYCVKV 125
             VEK+    F ++Q    +    ++S WN  +  L+ L  G+DW  F K    L  V +
Sbjct: 443 HKVEKLEPSNFHITQVEAHHIAHSVSSTWNSLVGVLKSLCRGNDWPLFFKVVFSLLVVSI 502

Query: 126 MLNLQFRMLMGLVLAFMFVVFIVYEQCEEEIDSLVAFA 163
           + ++  +    + ++  F+ F  YE+ E+ ID LV  A
Sbjct: 503 LSSMSSQSAFKIGISMAFLGFKAYEKWEDTIDDLVGNA 540



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.330    0.142    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,420,898
Number of extensions: 178638
Number of successful extensions: 528
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 529
Number of HSP's successfully gapped: 2
Length of query: 187
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 92
Effective length of database: 12,075,471
Effective search space: 1110943332
Effective search space used: 1110943332
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 153 (63.5 bits)