BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0512200 Os09g0512200|AK065469
         (65 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0512200  Similar to AMP-binding protein                      127   1e-30
Os03g0845500  Similar to Acyl-CoA synthetase-like protein          93   5e-20
>Os09g0512200 Similar to AMP-binding protein
          Length = 65

 Score =  127 bits (320), Expect = 1e-30,   Method: Compositional matrix adjust.
 Identities = 65/65 (100%), Positives = 65/65 (100%)

Query: 1  DSLQGIVLLSNPVKPSFIVHMVNWLLARIVAALLWSLHNLAKKLVFKKIHSAIGIPKVLG 60
          DSLQGIVLLSNPVKPSFIVHMVNWLLARIVAALLWSLHNLAKKLVFKKIHSAIGIPKVLG
Sbjct: 1  DSLQGIVLLSNPVKPSFIVHMVNWLLARIVAALLWSLHNLAKKLVFKKIHSAIGIPKVLG 60

Query: 61 TVGTQ 65
          TVGTQ
Sbjct: 61 TVGTQ 65
>Os03g0845500 Similar to Acyl-CoA synthetase-like protein
          Length = 750

 Score = 92.8 bits (229), Expect = 5e-20,   Method: Composition-based stats.
 Identities = 44/54 (81%), Positives = 48/54 (88%)

Query: 4   QGIVLLSNPVKPSFIVHMVNWLLARIVAALLWSLHNLAKKLVFKKIHSAIGIPK 57
           +G VL +NPVKPSFIV+MVNWL ARIVAALLW LHNLAK LV+KKIHSAIGI K
Sbjct: 429 EGTVLSNNPVKPSFIVYMVNWLSARIVAALLWPLHNLAKTLVYKKIHSAIGISK 482
  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.139    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,036,893
Number of extensions: 56304
Number of successful extensions: 125
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 125
Number of HSP's successfully gapped: 2
Length of query: 65
Length of database: 17,035,801
Length adjustment: 37
Effective length of query: 28
Effective length of database: 15,103,883
Effective search space: 422908724
Effective search space used: 422908724
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)