BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0507600 Os09g0507600|J080312K09
         (57 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0507600  Conserved hypothetical protein                      117   2e-27
Os08g0532900  emp24/gp25L/p24 family protein                       74   3e-14

>Os09g0507600 Conserved hypothetical protein
          Length = 57

 Score =  117 bits (293), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 57/57 (100%), Positives = 57/57 (100%)

Query: 1  MWLIPLLWKYVEHFSALFEQIAKLDEALYNIQFEQHWLEAQTDRQAICMFLVSYSLL 57
          MWLIPLLWKYVEHFSALFEQIAKLDEALYNIQFEQHWLEAQTDRQAICMFLVSYSLL
Sbjct: 1  MWLIPLLWKYVEHFSALFEQIAKLDEALYNIQFEQHWLEAQTDRQAICMFLVSYSLL 57



>Os08g0532900 emp24/gp25L/p24 family protein
          Length = 212

 Score = 73.6 bits (179), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 34/36 (94%), Positives = 35/36 (97%)

Query: 12  EHFSALFEQIAKLDEALYNIQFEQHWLEAQTDRQAI 47
           EHF+ LFEQIAKLDEALYNIQFEQHWLEAQTDRQAI
Sbjct: 133 EHFAPLFEQIAKLDEALYNIQFEQHWLEAQTDRQAI 168



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.330    0.138    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,753,744
Number of extensions: 43001
Number of successful extensions: 130
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 130
Number of HSP's successfully gapped: 2
Length of query: 57
Length of database: 17,035,801
Length adjustment: 30
Effective length of query: 27
Effective length of database: 15,469,381
Effective search space: 417673287
Effective search space used: 417673287
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 149 (62.0 bits)