BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0464000 Os09g0464000|AK069530
         (306 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0464000  Similar to Carbonate dehydratase-like protein       586   e-168
Os01g0639900  Similar to Carbonic anhydrase, chloroplast pre...   158   5e-39
Os01g0640132  Similar to Carbonic anhydrase, chloroplast pre...   103   2e-22

>Os09g0464000 Similar to Carbonate dehydratase-like protein
          Length = 306

 Score =  586 bits (1511), Expect = e-168,   Method: Compositional matrix adjust.
 Identities = 281/306 (91%), Positives = 281/306 (91%)

Query: 1   MAPSLLRPASPCLNLAPPTADGPGRSRSAVTIGGSRPXXXXXXXXXXXXXDFPCTTMASR 60
           MAPSLLRPASPCLNLAPPTADGPGRSRSAVTIGGSRP             DFPCTTMASR
Sbjct: 1   MAPSLLRPASPCLNLAPPTADGPGRSRSAVTIGGSRPLSVSLRVGGSSRRDFPCTTMASR 60

Query: 61  DHSGLTRQLLDFQHGTVDEIDGEHDPFMELKARFMDFKHRNCVDNISNYQNLAQQQTPKF 120
           DHSGLTRQLLDFQHGTVDEIDGEHDPFMELKARFMDFKHRNCVDNISNYQNLAQQQTPKF
Sbjct: 61  DHSGLTRQLLDFQHGTVDEIDGEHDPFMELKARFMDFKHRNCVDNISNYQNLAQQQTPKF 120

Query: 121 MVVACADSRVCPSSVLGFQPGEAFTVRNIANLVPPYQHGASETSAALEFAXXXXXXXXXX 180
           MVVACADSRVCPSSVLGFQPGEAFTVRNIANLVPPYQHGASETSAALEFA          
Sbjct: 121 MVVACADSRVCPSSVLGFQPGEAFTVRNIANLVPPYQHGASETSAALEFAVNTLEVENVL 180

Query: 181 XXGHSRCGGIQALMSMKSKQDDSQSRSFIRDWVSIAKSARLSTEAAAGNLNFELQCKHCE 240
             GHSRCGGIQALMSMKSKQDDSQSRSFIRDWVSIAKSARLSTEAAAGNLNFELQCKHCE
Sbjct: 181 VVGHSRCGGIQALMSMKSKQDDSQSRSFIRDWVSIAKSARLSTEAAAGNLNFELQCKHCE 240

Query: 241 KESINSSLLNLLTYPWIEKRVNEGTLSLHGGYYNFIDCTFEKWKLVYRQGLEGGSKYAIK 300
           KESINSSLLNLLTYPWIEKRVNEGTLSLHGGYYNFIDCTFEKWKLVYRQGLEGGSKYAIK
Sbjct: 241 KESINSSLLNLLTYPWIEKRVNEGTLSLHGGYYNFIDCTFEKWKLVYRQGLEGGSKYAIK 300

Query: 301 NRTTWS 306
           NRTTWS
Sbjct: 301 NRTTWS 306



>Os01g0639900 Similar to Carbonic anhydrase, chloroplast precursor (EC 4.2.1.1)
           (Carbonate dehydratase)
          Length = 272

 Score =  158 bits (399), Expect = 5e-39,   Method: Compositional matrix adjust.
 Identities = 84/203 (41%), Positives = 116/203 (57%), Gaps = 14/203 (6%)

Query: 85  DPFMELKARFMDFKHRNCVDNISNYQNLAQQQTPKFMVVACADSRVCPSSVLGFQPGEAF 144
           D F + K  F D K          ++ L   Q PK+MV +CADSRVCPS  +G +PGEAF
Sbjct: 80  DGFAKFKTEFYDKKPEL-------FEPLKAGQAPKYMVFSCADSRVCPSVTMGLEPGEAF 132

Query: 145 TVRNIANLVPPY---QHGASETSAALEFAXXXXXXXXXXXXGHSRCGGIQALMSMKSKQD 201
           TVRNIAN+VP Y   +H      +A+E+A            GHSRCGGI+AL+S+K    
Sbjct: 133 TVRNIANMVPAYCKIKHAG--VGSAIEYAVCALKVELIVVIGHSRCGGIKALLSLKDGAP 190

Query: 202 DSQSRSFIRDWVSIAKSARLSTEAAAGNLNFELQCKHCEKESINSSLLNLLTYPWIEKRV 261
           D  S  F+ DWV     A+   +    +L F+ QC   EKE++N SL NL TYP++++ +
Sbjct: 191 D--SFHFVEDWVRTGFPAKKKVQTEHASLPFDDQCAILEKEAVNQSLENLKTYPFVKEGI 248

Query: 262 NEGTLSLHGGYYNFIDCTFEKWK 284
             GTL L GG+Y+F+    + W+
Sbjct: 249 ANGTLKLVGGHYDFVSGNLDLWE 271



>Os01g0640132 Similar to Carbonic anhydrase, chloroplast precursor (EC 4.2.1.1)
           (Carbonate dehydratase)
          Length = 183

 Score =  103 bits (256), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 54/126 (42%), Positives = 72/126 (57%), Gaps = 3/126 (2%)

Query: 121 MVVACADSRVCPSSVLGFQPGEAFTVRNIANLVPPY-QHGASETSAALEFAXXXXXXXXX 179
           MV +CADSRVCP+   G QPGEAFTVRNIA++VP Y + G     +A+E+A         
Sbjct: 1   MVFSCADSRVCPTLTFGLQPGEAFTVRNIASMVPAYDKRGQCSIGSAIEYAVVVLKVECI 60

Query: 180 XXXGHSRCGGIQALMSMKSKQDDSQSRSFIRDWVSIAKSARLSTEAAAGNLNFELQCKHC 239
              GHS CGGI+ L+S+  K+D   +  F+ DWV I  +A+   E     L F+ QC   
Sbjct: 61  IVIGHSCCGGIKELLSL--KEDRPNTFHFVDDWVKIGLAAKKKVERENMLLPFDDQCTVL 118

Query: 240 EKESIN 245
           EK  + 
Sbjct: 119 EKVEVK 124



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.132    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,000,995
Number of extensions: 355688
Number of successful extensions: 785
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 781
Number of HSP's successfully gapped: 3
Length of query: 306
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 205
Effective length of database: 11,762,187
Effective search space: 2411248335
Effective search space used: 2411248335
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)