BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0446200 Os09g0446200|011-092-H04
         (544 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0446200  Spc97/Spc98 family protein                         1009   0.0  
Os04g0501700  Spc97/Spc98 family protein                          108   7e-24

>Os09g0446200 Spc97/Spc98 family protein
          Length = 544

 Score = 1009 bits (2610), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 498/544 (91%), Positives = 498/544 (91%)

Query: 1   DSGVSGNYLSLRRLAVWLAEPAVRMRLMAVLVDGCRGLRXXXXXXXXXXXXXXXDPTFQD 60
           DSGVSGNYLSLRRLAVWLAEPAVRMRLMAVLVDGCRGLR               DPTFQD
Sbjct: 1   DSGVSGNYLSLRRLAVWLAEPAVRMRLMAVLVDGCRGLRGGAMAGAIHGHAQHGDPTFQD 60

Query: 61  FMGRLLRRVCSPLFEMVRSWVLEGELEDVFAEFFIVGQPVKAESLWQEGYLLQSDMLPAF 120
           FMGRLLRRVCSPLFEMVRSWVLEGELEDVFAEFFIVGQPVKAESLWQEGYLLQSDMLPAF
Sbjct: 61  FMGRLLRRVCSPLFEMVRSWVLEGELEDVFAEFFIVGQPVKAESLWQEGYLLQSDMLPAF 120

Query: 121 ISPVLAQRILRTGKSINFLKVCCDDNGWADAATEAAVCVGTTTSRGGLGYGQIDALEALV 180
           ISPVLAQRILRTGKSINFLKVCCDDNGWADAATEAAVCVGTTTSRGGLGYGQIDALEALV
Sbjct: 121 ISPVLAQRILRTGKSINFLKVCCDDNGWADAATEAAVCVGTTTSRGGLGYGQIDALEALV 180

Query: 181 VEAAKRIDRHLMDVIHKRYRFKDHCLAIKRYLLLGQGDFVQYLMDVVGPELSEPANRISS 240
           VEAAKRIDRHLMDVIHKRYRFKDHCLAIKRYLLLGQGDFVQYLMDVVGPELSEPANRISS
Sbjct: 181 VEAAKRIDRHLMDVIHKRYRFKDHCLAIKRYLLLGQGDFVQYLMDVVGPELSEPANRISS 240

Query: 241 FHLAGLLETAIRASNAQYDDRDILDRIKVKMMDHGDGDRGWDVFSLEYDARVPLDTVFTA 300
           FHLAGLLETAIRASNAQYDDRDILDRIKVKMMDHGDGDRGWDVFSLEYDARVPLDTVFTA
Sbjct: 241 FHLAGLLETAIRASNAQYDDRDILDRIKVKMMDHGDGDRGWDVFSLEYDARVPLDTVFTA 300

Query: 301 SVMKRYLKIFNFLWKLKRVDHSLTGIWKTMKPNCIVSSPFYKEGTNIRSQFVSVLRKCQV 360
           SVMKRYLKIFNFLWKLKRVDHSLTGIWKTMKPNCIVSSPFYKEGTNIRSQFVSVLRKCQV
Sbjct: 301 SVMKRYLKIFNFLWKLKRVDHSLTGIWKTMKPNCIVSSPFYKEGTNIRSQFVSVLRKCQV 360

Query: 361 LFNEMNHFVTNFQYYIMFEVLEVSWARFSEEMXXXXXXXXXXXXXXXXXTSIVEKSLLGE 420
           LFNEMNHFVTNFQYYIMFEVLEVSWARFSEEM                 TSIVEKSLLGE
Sbjct: 361 LFNEMNHFVTNFQYYIMFEVLEVSWARFSEEMDSAKDLDDLLLAHDKYLTSIVEKSLLGE 420

Query: 421 RSHGILRNLFALFDIILQFRSHADRWFERIYELQLRXXXXXXXXXXXXXXEVDSWVDGGR 480
           RSHGILRNLFALFDIILQFRSHADRWFERIYELQLR              EVDSWVDGGR
Sbjct: 421 RSHGILRNLFALFDIILQFRSHADRWFERIYELQLRGKGKPKSKAKAKSKEVDSWVDGGR 480

Query: 481 KAMIQLAGELFRKMGEDLDSIAKDYTSSLDAFIAQLPMQQHVDLKFLLFRLDFTEYYSRV 540
           KAMIQLAGELFRKMGEDLDSIAKDYTSSLDAFIAQLPMQQHVDLKFLLFRLDFTEYYSRV
Sbjct: 481 KAMIQLAGELFRKMGEDLDSIAKDYTSSLDAFIAQLPMQQHVDLKFLLFRLDFTEYYSRV 540

Query: 541 SSNK 544
           SSNK
Sbjct: 541 SSNK 544



>Os04g0501700 Spc97/Spc98 family protein
          Length = 711

 Score =  108 bits (271), Expect = 7e-24,   Method: Compositional matrix adjust.
 Identities = 96/369 (26%), Positives = 158/369 (42%), Gaps = 64/369 (17%)

Query: 55  DPTFQDFMGRLLRRVCSPLFEMVRSWVLEGELEDVFAEFFIV-GQPVKAESL-------- 105
           D   +  + ++     +    M+  WV EG ++D + EFFI   + ++ ESL        
Sbjct: 223 DSAVRSLLEKMTECASAAYLRMLERWVYEGVIDDPYGEFFIAENKSLQKESLTQDYDAKY 282

Query: 106 WQEGYLLQSDMLPAFISPVLAQRILRTGKSINFLKVCCDDNGWADAATEAAVCVGTTTSR 165
           WQ+ Y L+ D +P+F++ V A  IL TGK +N ++ C           E  V V  + S 
Sbjct: 283 WQQRYSLK-DGIPSFLTNV-AATILTTGKYLNVMREC-----------EYTVQVPLSESS 329

Query: 166 GGLGYGQIDALEALVVEAAKRIDRHLMDVIHKRYRFKDHCLAIKRYLLLGQGDFVQYLMD 225
             +G+G        +  A       L+ ++  +Y       ++KRYLLL QGDF+ + MD
Sbjct: 330 KLMGFGSNHQYLECIKSAYDFASGELLTLMKDKYDLIGKLRSLKRYLLLDQGDFLVHFMD 389

Query: 226 VVGPELSEPANRISSFHLAGLLETAIRASNAQYD----------DRDIL----------- 264
           +   EL++    IS   L  L++ A+R++ A  D          +R  L           
Sbjct: 390 IAREELTKKPEEISVEKLQSLVDIALRSTAAASDPSHEDLTCCVERSSLLKKLSTLKDLD 449

Query: 265 -----DRIKVKMMDHGD--GDRGWDVFSLEYDARVPLDTVFTASVMKRYLKIFNFLWKLK 317
                D++    +DH       G + F L Y  + PL  V +   + +Y  IF  L+  K
Sbjct: 450 CAYPSDKLVAADVDHPMPLSVTGLETFCLSYKVQWPLSLVISRKSLTKYQLIFRLLFHCK 509

Query: 318 RVDHSLTGIWKTMKPNCIVSSPFYKEG-TNIRSQFVSVLRKCQVLFNEMNHFVTNFQYYI 376
            V   L   W+             ++G  +++     VLR   +L   M  FV +  +Y+
Sbjct: 510 HVSRQLCTAWQI------------QQGFRSVKILGTPVLRS-SILCRSMLKFVNSLLHYL 556

Query: 377 MFEVLEVSW 385
            FEVLE +W
Sbjct: 557 TFEVLEPNW 565



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.140    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 16,040,597
Number of extensions: 593982
Number of successful extensions: 1453
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1452
Number of HSP's successfully gapped: 2
Length of query: 544
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 438
Effective length of database: 11,501,117
Effective search space: 5037489246
Effective search space used: 5037489246
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 158 (65.5 bits)