BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os09g0412400 Os09g0412400|AK073672
(83 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os09g0412400 Conserved hypothetical protein 136 3e-33
Os09g0415200 108 1e-24
>Os09g0412400 Conserved hypothetical protein
Length = 83
Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust.
Identities = 69/83 (83%), Positives = 69/83 (83%)
Query: 1 MAKPRXXXXXXXXXXXXXXSFSHGEAKDVQLVKPARRYGRWRSALQAGDGMVSTVADYSD 60
MAKPR SFSHGEAKDVQLVKPARRYGRWRSALQAGDGMVSTVADYSD
Sbjct: 1 MAKPRLLALLLLAALLLALSFSHGEAKDVQLVKPARRYGRWRSALQAGDGMVSTVADYSD 60
Query: 61 PKPNTNPRGGVLPPTDPNSPPAH 83
PKPNTNPRGGVLPPTDPNSPPAH
Sbjct: 61 PKPNTNPRGGVLPPTDPNSPPAH 83
>Os09g0415200
Length = 86
Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust.
Identities = 54/71 (76%), Positives = 57/71 (80%), Gaps = 7/71 (9%)
Query: 20 SFSHG-------EAKDVQLVKPARRYGRWRSALQAGDGMVSTVADYSDPKPNTNPRGGVL 72
SFSHG +A+DVQLVK RRY RWRS QAG GMVSTVADYSDPK NTNPRGGVL
Sbjct: 16 SFSHGVVVATVAKARDVQLVKLVRRYVRWRSVFQAGIGMVSTVADYSDPKTNTNPRGGVL 75
Query: 73 PPTDPNSPPAH 83
PPT+PNSPPAH
Sbjct: 76 PPTNPNSPPAH 86
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.312 0.131 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,284,599
Number of extensions: 129448
Number of successful extensions: 471
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 471
Number of HSP's successfully gapped: 2
Length of query: 83
Length of database: 17,035,801
Length adjustment: 53
Effective length of query: 30
Effective length of database: 14,268,459
Effective search space: 428053770
Effective search space used: 428053770
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 149 (62.0 bits)