BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0396900 Os09g0396900|AK071589
         (166 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0396900  Protein of unknown function DUF125, transmembr...   300   3e-82
Os04g0463400  Protein of unknown function DUF125, transmembr...   190   4e-49

>Os09g0396900 Protein of unknown function DUF125, transmembrane family protein
          Length = 166

 Score =  300 bits (769), Expect = 3e-82,   Method: Compositional matrix adjust.
 Identities = 149/166 (89%), Positives = 149/166 (89%)

Query: 1   MVKEFVQDEEKQRLLLDEHTEKHFTAGEVAAEIADILSQYGLGPEEYGPVVNSLRSNPKA 60
           MVKEFVQDEEKQRLLLDEHTEKHFTAGEVAAEIADILSQYGLGPEEYGPVVNSLRSNPKA
Sbjct: 1   MVKEFVQDEEKQRLLLDEHTEKHFTAGEVAAEIADILSQYGLGPEEYGPVVNSLRSNPKA 60

Query: 61  WLEFMMKFELGLEKPEPRRALMSAGTIALAYVVGGLVPLLPYMFVPTADRAMATSVVVTL 120
           WLEFMMKFELGLEKPEPRRALMSAGTIALAYVVGGLVPLLPYMFVPTADRAMATSVVVTL
Sbjct: 61  WLEFMMKFELGLEKPEPRRALMSAGTIALAYVVGGLVPLLPYMFVPTADRAMATSVVVTL 120

Query: 121 AALLFFGYVKGRFTGNRPFISAFQTXXXXXXXXXXXXXXXXXVQSI 166
           AALLFFGYVKGRFTGNRPFISAFQT                 VQSI
Sbjct: 121 AALLFFGYVKGRFTGNRPFISAFQTAVIGALASAAAFGMAKAVQSI 166



>Os04g0463400 Protein of unknown function DUF125, transmembrane family protein
          Length = 252

 Score =  190 bits (483), Expect = 4e-49,   Method: Compositional matrix adjust.
 Identities = 92/139 (66%), Positives = 109/139 (78%)

Query: 7   QDEEKQRLLLDEHTEKHFTAGEVAAEIADILSQYGLGPEEYGPVVNSLRSNPKAWLEFMM 66
           + +  QR +  E  E        AAEI +I+SQYGL P EYGPVV+ LR NP+AWL+FMM
Sbjct: 93  EADHYQREMKREQEEIIAVPDTEAAEIGEIMSQYGLEPHEYGPVVDGLRRNPQAWLDFMM 152

Query: 67  KFELGLEKPEPRRALMSAGTIALAYVVGGLVPLLPYMFVPTADRAMATSVVVTLAALLFF 126
           +FELGLEKP+P+RA+ SA TIAL+YV+GGLVPLLPYMF+ TA  AM TSV VTL ALLFF
Sbjct: 153 RFELGLEKPDPKRAIQSALTIALSYVIGGLVPLLPYMFISTAQNAMLTSVGVTLVALLFF 212

Query: 127 GYVKGRFTGNRPFISAFQT 145
           GY+KGRFTGNRPF+SA QT
Sbjct: 213 GYIKGRFTGNRPFLSAVQT 231



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,735,215
Number of extensions: 178572
Number of successful extensions: 409
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 409
Number of HSP's successfully gapped: 2
Length of query: 166
Length of database: 17,035,801
Length adjustment: 93
Effective length of query: 73
Effective length of database: 12,179,899
Effective search space: 889132627
Effective search space used: 889132627
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 152 (63.2 bits)