BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0297000 Os09g0297000|AK068174
         (482 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0297000  Similar to Ferrochelatase II, chloroplast prec...   921   0.0  
Os05g0361200  Similar to Ferrochelatase                           495   e-140

>Os09g0297000 Similar to Ferrochelatase II, chloroplast precursor (EC 4.99.1.1)
           (Protoheme ferro-lyase) (Heme synthetase)
          Length = 482

 Score =  921 bits (2380), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 453/482 (93%), Positives = 453/482 (93%)

Query: 1   MECVRSGSGVLDPRCSPRFLGKKGGSLTSCGKATSTNLAICTKHEQNLHGNVKPSQLAAS 60
           MECVRSGSGVLDPRCSPRFLGKKGGSLTSCGKATSTNLAICTKHEQNLHGNVKPSQLAAS
Sbjct: 1   MECVRSGSGVLDPRCSPRFLGKKGGSLTSCGKATSTNLAICTKHEQNLHGNVKPSQLAAS 60

Query: 61  GSSYSVHRSPVLKQRQNLSARSTSADVYTTFDENVRAVSSHAAEEKVGVLLLNLGGPETL 120
           GSSYSVHRSPVLKQRQNLSARSTSADVYTTFDENVRAVSSHAAEEKVGVLLLNLGGPETL
Sbjct: 61  GSSYSVHRSPVLKQRQNLSARSTSADVYTTFDENVRAVSSHAAEEKVGVLLLNLGGPETL 120

Query: 121 DDVQPFLFNLFADPDIIXXXXXXXXXXXXXAKLISTFRAPKSKEGYASIGGGSPLRKITD 180
           DDVQPFLFNLFADPDII             AKLISTFRAPKSKEGYASIGGGSPLRKITD
Sbjct: 121 DDVQPFLFNLFADPDIIRLPRLFRFLQRPLAKLISTFRAPKSKEGYASIGGGSPLRKITD 180

Query: 181 EQXXXXXXXXXXXXXXXXIYVGMRYWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSG 240
           EQ                IYVGMRYWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSG
Sbjct: 181 EQANALKVALKKKNLNANIYVGMRYWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSG 240

Query: 241 SSIRVLQNIVKEDSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSA 300
           SSIRVLQNIVKEDSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSA
Sbjct: 241 SSIRVLQNIVKEDSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSA 300

Query: 301 HGVPLTYVTDAGDPYRDQMEDCIALIMGELKSRGILNSHTLAYQSRVGPVQWLKPYTDEV 360
           HGVPLTYVTDAGDPYRDQMEDCIALIMGELKSRGILNSHTLAYQSRVGPVQWLKPYTDEV
Sbjct: 301 HGVPLTYVTDAGDPYRDQMEDCIALIMGELKSRGILNSHTLAYQSRVGPVQWLKPYTDEV 360

Query: 361 LVELGQQGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIENWGRVPALGCTSSFIS 420
           LVELGQQGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIENWGRVPALGCTSSFIS
Sbjct: 361 LVELGQQGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIENWGRVPALGCTSSFIS 420

Query: 421 DLADAVVEALPSASALVTKKVDESDSDMDLMHYLSKMFFGSILAFVLLLSPRLISAFRNT 480
           DLADAVVEALPSASALVTKKVDESDSDMDLMHYLSKMFFGSILAFVLLLSPRLISAFRNT
Sbjct: 421 DLADAVVEALPSASALVTKKVDESDSDMDLMHYLSKMFFGSILAFVLLLSPRLISAFRNT 480

Query: 481 LL 482
           LL
Sbjct: 481 LL 482



>Os05g0361200 Similar to Ferrochelatase
          Length = 526

 Score =  495 bits (1274), Expect = e-140,   Method: Compositional matrix adjust.
 Identities = 246/430 (57%), Positives = 304/430 (70%), Gaps = 11/430 (2%)

Query: 13  PRCSPRFLGKKGGSLTSCGKATSTNLAICTKHEQNLHGNVKPSQLAASGSSYSVHRSPVL 72
           PR S R    +  ++ SC  ++ T L +         G     + +A          P+ 
Sbjct: 31  PRNSSRVNLSRTYAIKSCSVSSRTGLCL---------GQCYHKKSSACKCKLGWSSQPLS 81

Query: 73  KQRQNLSARSTSADVYTTFDENVRAVSSHAAEEKVGVLLLNLGGPETLDDVQPFLFNLFA 132
             R +L   S++++   T   +   +      EK+GVLLLNLGGPETLDDVQPFLFNLFA
Sbjct: 82  SLRHHLRVHSSASEAVLTSQSDFTKLL--VGNEKIGVLLLNLGGPETLDDVQPFLFNLFA 139

Query: 133 DPDIIXXXXXXXXXXXXXAKLISTFRAPKSKEGYASIGGGSPLRKITDEQXXXXXXXXXX 192
           DPDII             A+ IS  RAPKSKEGYASIGGGSPLR+ITD Q          
Sbjct: 140 DPDIIRLPRLFRFLQKPLAQFISVVRAPKSKEGYASIGGGSPLRQITDAQAEALRKALCD 199

Query: 193 XXXXXXIYVGMRYWYPFTEEAIDQIKKDKITKLVVLPLYPQYSISTSGSSIRVLQNIVKE 252
                 +YVGMRYW+PFTEEAI+QIK+D ITKLVVLPLYPQ+SISTSGSS+R+L+ I +E
Sbjct: 200 KDIPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQFSISTSGSSLRLLEGIFRE 259

Query: 253 DSYFAGLPISIIESWYQRDGYVKSMADLIEKELSIFSNPEEVMIFFSAHGVPLTYVTDAG 312
           D Y   +  ++I SWYQR+GY+K+MA LIEKEL  FS P++VMIFFSAHGVPL YV +AG
Sbjct: 260 DEYLVNMQHTVIPSWYQREGYIKAMATLIEKELRTFSEPQKVMIFFSAHGVPLAYVEEAG 319

Query: 313 DPYRDQMEDCIALIMGELKSRGILNSHTLAYQSRVGPVQWLKPYTDEVLVELGQQGVKSL 372
           DPY+ +ME+C+ LIM EL+ RGI NS TLAYQSRVGPV+WL+PYTDE ++ELGQ+GVKSL
Sbjct: 320 DPYKAEMEECVDLIMEELEKRGITNSCTLAYQSRVGPVEWLRPYTDETIIELGQKGVKSL 379

Query: 373 LAVPVSFVSEHIETLEEIDMEYKELALESGIENWGRVPALGCTSSFISDLADAVVEALPS 432
           LAVP+SFVSEHIETLEEID+EYKELALESGI++WGRVPALGC  +FI+DLADAV+E+LP 
Sbjct: 380 LAVPISFVSEHIETLEEIDVEYKELALESGIKHWGRVPALGCEPTFITDLADAVIESLPY 439

Query: 433 ASALVTKKVD 442
             A+    ++
Sbjct: 440 VGAMAVSNLE 449



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,970,070
Number of extensions: 571497
Number of successful extensions: 1507
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1505
Number of HSP's successfully gapped: 2
Length of query: 482
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 377
Effective length of database: 11,553,331
Effective search space: 4355605787
Effective search space used: 4355605787
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)