BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0248000 Os09g0248000|AK065068
         (176 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0248000  Protein of unknown function DUF516 family protein   357   3e-99
Os08g0339200  Protein of unknown function DUF516 family protein   254   2e-68

>Os09g0248000 Protein of unknown function DUF516 family protein
          Length = 176

 Score =  357 bits (915), Expect = 3e-99,   Method: Compositional matrix adjust.
 Identities = 176/176 (100%), Positives = 176/176 (100%)

Query: 1   MVVLVVATASDPASIGPAAAFLAMPGWSPGPPIPEGMESFTNGDVRLLKHERSIIAEDDL 60
           MVVLVVATASDPASIGPAAAFLAMPGWSPGPPIPEGMESFTNGDVRLLKHERSIIAEDDL
Sbjct: 1   MVVLVVATASDPASIGPAAAFLAMPGWSPGPPIPEGMESFTNGDVRLLKHERSIIAEDDL 60

Query: 61  DNRWQAATGEAVSEVIFLSKHTAVSNRPALTVHPIGVPHLREDETPPQGGRPGWAALPNP 120
           DNRWQAATGEAVSEVIFLSKHTAVSNRPALTVHPIGVPHLREDETPPQGGRPGWAALPNP
Sbjct: 61  DNRWQAATGEAVSEVIFLSKHTAVSNRPALTVHPIGVPHLREDETPPQGGRPGWAALPNP 120

Query: 121 RIGPWLRLMQKIAVDQGLVPEFEITLEATHHGPVTNTPTMFVEIGIDLLPCLYSLY 176
           RIGPWLRLMQKIAVDQGLVPEFEITLEATHHGPVTNTPTMFVEIGIDLLPCLYSLY
Sbjct: 121 RIGPWLRLMQKIAVDQGLVPEFEITLEATHHGPVTNTPTMFVEIGIDLLPCLYSLY 176



>Os08g0339200 Protein of unknown function DUF516 family protein
          Length = 316

 Score =  254 bits (650), Expect = 2e-68,   Method: Compositional matrix adjust.
 Identities = 132/167 (79%), Positives = 147/167 (88%), Gaps = 2/167 (1%)

Query: 1   MVVLVVATASDPASIGPAAAFLAMPGWSPGPPIPEGMESFTNG-DVRLLKHERSIIAEDD 59
           MVVLVVAT SD AS  PAAAFLAMPGW+PGP IP+G+ESF NG  VRLL+H R I+ EDD
Sbjct: 1   MVVLVVATTSDAASATPAAAFLAMPGWTPGPSIPDGVESFANGAGVRLLRHARGIVEEDD 60

Query: 60  LDNRWQAATGEAVSEVIFLSKHTAVSNRPALTVHPIGVPHLRED-ETPPQGGRPGWAALP 118
           LD RW+AATGE VSEV+ LS+HTAVSNRPALTVHPIGVPHLR+  + PPQGGRPGWAA P
Sbjct: 61  LDLRWEAATGERVSEVVLLSRHTAVSNRPALTVHPIGVPHLRDGVDVPPQGGRPGWAAPP 120

Query: 119 NPRIGPWLRLMQKIAVDQGLVPEFEITLEATHHGPVTNTPTMFVEIG 165
           NPRIGPWLRL+++IA D+GLVPEFEITLE THHGPVTNTPT+FVEIG
Sbjct: 121 NPRIGPWLRLLRRIAADRGLVPEFEITLEGTHHGPVTNTPTLFVEIG 167



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.137    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,371,347
Number of extensions: 340078
Number of successful extensions: 1148
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1146
Number of HSP's successfully gapped: 2
Length of query: 176
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 82
Effective length of database: 12,127,685
Effective search space: 994470170
Effective search space used: 994470170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 152 (63.2 bits)