BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0103500 Os09g0103500|AK065573
         (448 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0103500  Pseudouridine synthase domain containing protein    773   0.0  
Os08g0520100  Pseudouridine synthase domain containing protein    200   1e-51

>Os09g0103500 Pseudouridine synthase domain containing protein
          Length = 448

 Score =  773 bits (1995), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 389/431 (90%), Positives = 389/431 (90%)

Query: 18  GVAPKPQWLATAARRGALVSGDDGGETGERGKSPWLQLPPFAPLXXXXXXXXXXXXXXXX 77
           GVAPKPQWLATAARRGALVSGDDGGETGERGKSPWLQLPPFAPL                
Sbjct: 18  GVAPKPQWLATAARRGALVSGDDGGETGERGKSPWLQLPPFAPLDAAAAARAISRGGGEG 77

Query: 78  XXXXXXXTAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSA 137
                  TAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSA
Sbjct: 78  GDGEQGATAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSA 137

Query: 138 KDQLMPGDILFLPVNLKESSVAEKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQ 197
           KDQLMPGDILFLPVNLKESSVAEKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQ
Sbjct: 138 KDQLMPGDILFLPVNLKESSVAEKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQ 197

Query: 198 GGVGIKNSIDVLASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTAD 257
           GGVGIKNSIDVLASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTAD
Sbjct: 198 GGVGIKNSIDVLASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTAD 257

Query: 258 ALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDA 317
           ALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDA
Sbjct: 258 ALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDA 317

Query: 318 LTEYRVIESCPQGYTWLELFPRTGRKHQRFLEPQLLGITSMDGKRIRSGCLFPCHGQLMR 377
           LTEYRVIESCPQGYTWLELFPRTGRKHQRFLEPQLLGITSMDGKRIRSGCLFPCHGQLMR
Sbjct: 318 LTEYRVIESCPQGYTWLELFPRTGRKHQRFLEPQLLGITSMDGKRIRSGCLFPCHGQLMR 377

Query: 378 NCSGKGSFPLGLLWVAEASLRSNLSFIYTASKWFFLMSQWLFIGCXXXXXXXXXXXXXXX 437
           NCSGKGSFPLGLLWVAEASLRSNLSFIYTASKWFFLMSQWLFIGC               
Sbjct: 378 NCSGKGSFPLGLLWVAEASLRSNLSFIYTASKWFFLMSQWLFIGCSLQTLILISQILRSS 437

Query: 438 XXXXHCRCICG 448
               HCRCICG
Sbjct: 438 TLLLHCRCICG 448



>Os08g0520100 Pseudouridine synthase domain containing protein
          Length = 475

 Score =  200 bits (509), Expect = 1e-51,   Method: Compositional matrix adjust.
 Identities = 105/272 (38%), Positives = 163/272 (59%), Gaps = 11/272 (4%)

Query: 85  TAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPG 144
           TA+ WV+    D+P  +VQ  F  R V       E+S     +++H L+++   D + PG
Sbjct: 80  TAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETIKSQKHHLKKIKHNDVMEPG 139

Query: 145 DILFLPVNLKESSVAEKTKKFD------NRNEINFLRGLEIYKDEAIIVVNKPPGMPVQG 198
             + LPV++ E  + ++ +         N++EI +LR L I+KD AI+V+NKPP +P++G
Sbjct: 140 MRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVIHKDSAILVLNKPPKVPMKG 199

Query: 199 GVGIKNSIDVLASM-FEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFRE---- 253
            + + NS+DVLA+      + E P+LVHRLDR+ SG+L+ GR + S + LH +F      
Sbjct: 200 NLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLFGRTKESFTRLHWLFTSVNLA 259

Query: 254 KTADALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAAS 313
           KT   + +       ++Y ALVIGTP+  +G++SAPL+K+LL DGK+ER+ +   S    
Sbjct: 260 KTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKVLLDDGKAERVILAHPSGIDG 319

Query: 314 VQDALTEYRVIESCPQGYTWLELFPRTGRKHQ 345
            Q+A+T YRV+     G +W+EL P TGRKHQ
Sbjct: 320 AQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQ 351



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,651,617
Number of extensions: 526372
Number of successful extensions: 1430
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1427
Number of HSP's successfully gapped: 2
Length of query: 448
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 344
Effective length of database: 11,605,545
Effective search space: 3992307480
Effective search space used: 3992307480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 158 (65.5 bits)