BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0566400 Os08g0566400|AK067804
         (398 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0566400  Ribokinase family protein                           613   e-176
Os10g0465900  PfkB domain containing protein                      188   6e-48

>Os08g0566400 Ribokinase family protein
          Length = 398

 Score =  613 bits (1581), Expect = e-176,   Method: Compositional matrix adjust.
 Identities = 314/398 (78%), Positives = 314/398 (78%)

Query: 1   MTMVRIIGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVLGCGAVSADYLAT 60
           MTMVRIIG                                     VVLGCGAVSADYLAT
Sbjct: 1   MTMVRIIGPSPAQSSSPTPRRPRPPRPRRRPPTMASAAASSSKPPVVLGCGAVSADYLAT 60

Query: 61  VASFPNPDDKIRSLTLKVQXXXXXXXXXXXXXXXXXXPRIISKVSNDPQGRNILKELQDD 120
           VASFPNPDDKIRSLTLKVQ                  PRIISKVSNDPQGRNILKELQDD
Sbjct: 61  VASFPNPDDKIRSLTLKVQGGGNTGNALTAAARLGLRPRIISKVSNDPQGRNILKELQDD 120

Query: 121 GVDTSHILVAEEGNSPFTYIIVDNQTKTRTCIHTPGYPPMVPEELTQENLFAALDGADIV 180
           GVDTSHILVAEEGNSPFTYIIVDNQTKTRTCIHTPGYPPMVPEELTQENLFAALDGADIV
Sbjct: 121 GVDTSHILVAEEGNSPFTYIIVDNQTKTRTCIHTPGYPPMVPEELTQENLFAALDGADIV 180

Query: 181 YFDVRLHETALLVAEEASQRKLPILIDAERKRDGLDELLNFASYVVCSAKFPQAWTGASS 240
           YFDVRLHETALLVAEEASQRKLPILIDAERKRDGLDELLNFASYVVCSAKFPQAWTGASS
Sbjct: 181 YFDVRLHETALLVAEEASQRKLPILIDAERKRDGLDELLNFASYVVCSAKFPQAWTGASS 240

Query: 241 TPVALVSMLLRLPNIKFIIVTLGEKGCLMLERSTTDASEAEEIDXXXXXXXXXXXXXXXX 300
           TPVALVSMLLRLPNIKFIIVTLGEKGCLMLERSTTDASEAEEID                
Sbjct: 241 TPVALVSMLLRLPNIKFIIVTLGEKGCLMLERSTTDASEAEEIDVESLLESLEKKEVLSS 300

Query: 301 XMPKCIASKSNLRISADXXXXXXXXXXXXTAEIIPSEELIDTTGAGDAFIGAVLYGLCSG 360
            MPKCIASKSNLRISAD            TAEIIPSEELIDTTGAGDAFIGAVLYGLCSG
Sbjct: 301 SMPKCIASKSNLRISADGIGSISGRLLLGTAEIIPSEELIDTTGAGDAFIGAVLYGLCSG 360

Query: 361 MPPEKMLPFAAQVAACGCRGLGARTALPHRTDPRLVAY 398
           MPPEKMLPFAAQVAACGCRGLGARTALPHRTDPRLVAY
Sbjct: 361 MPPEKMLPFAAQVAACGCRGLGARTALPHRTDPRLVAY 398



>Os10g0465900 PfkB domain containing protein
          Length = 223

 Score =  188 bits (477), Expect = 6e-48,   Method: Compositional matrix adjust.
 Identities = 101/222 (45%), Positives = 135/222 (60%), Gaps = 6/222 (2%)

Query: 182 FDVRLHETALLVAE--EASQRKLPILIDAE--RKRDGLDELLNFASYVVCSAKFPQAWTG 237
            D+++     L++   +A Q K+PIL+DAE  R R+ L  LL+ ASY+VC+ KFP+ WT 
Sbjct: 1   MDIQMRWPWQLLSRNVQADQMKIPILVDAEPERTREELGGLLSLASYIVCNGKFPEKWTS 60

Query: 238 ASSTPVALVSMLLRLPNIKFIIVTLGEKGCLMLERSTT-DASEAEEIDXXXXXXXXXXXX 296
             S P AL+ +LL+ P   F +VTLGE GC+MLER    +  E E +D            
Sbjct: 61  VPSIPSALLEILLQYPRACFAVVTLGENGCMMLERGKDGENYETEPVDIENVAESLRLKV 120

Query: 297 XXXXXMPKCIASKSNLRISADXXXXXXXXXXXXTAEIIPSEELIDTTGAGDAFIGAVLYG 356
                +P C++SK  +R++A             TAE IP+ EL+DTTG GDAFIGAVL+G
Sbjct: 121 DKDDILPTCVSSKF-MRLTARGSGNIFARLLIGTAESIPASELVDTTGCGDAFIGAVLHG 179

Query: 357 LCSGMPPEKMLPFAAQVAACGCRGLGARTALPHRTDPRLVAY 398
           L + MPPEKMLPFA QVA   CR +GAR+ LP ++DPRL  Y
Sbjct: 180 LSTEMPPEKMLPFACQVAGIKCRAIGARSGLPWQSDPRLTKY 221



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,612,546
Number of extensions: 374586
Number of successful extensions: 780
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 776
Number of HSP's successfully gapped: 2
Length of query: 398
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 295
Effective length of database: 11,657,759
Effective search space: 3439038905
Effective search space used: 3439038905
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)