BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0550100 Os08g0550100|AK061287
         (486 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0550100  Similar to 26S proteasome subunit RPN3a             838   0.0  
Os09g0541900  Similar to 26S proteasome subunit RPN3a             775   0.0  
AK108887                                                          293   2e-79

>Os08g0550100 Similar to 26S proteasome subunit RPN3a
          Length = 486

 Score =  838 bits (2164), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 417/486 (85%), Positives = 417/486 (85%)

Query: 1   MTDVQMSDSEXXXXXXXXXXXXXLHHLKEIASVIEAGSLSKEVRRISXXXXXXXXXXXXX 60
           MTDVQMSDSE             LHHLKEIASVIEAGSLSKEVRRIS             
Sbjct: 1   MTDVQMSDSEPQAAPPAAPAASALHHLKEIASVIEAGSLSKEVRRISRAVRLTVALRRRL 60

Query: 61  XXXXXXXFLAFALPPSSEAYTRLTSLVPKEDDTEMDIDMAAPATQISIKHGLPEIEIYCY 120
                  FLAFALPPSSEAYTRLTSLVPKEDDTEMDIDMAAPATQISIKHGLPEIEIYCY
Sbjct: 61  AARDVSAFLAFALPPSSEAYTRLTSLVPKEDDTEMDIDMAAPATQISIKHGLPEIEIYCY 120

Query: 121 LLVLIFLIDQKKYDDAKACANASIARLKNLNRRTVDVLASRLYFYYSYVHELTNSLAEIR 180
           LLVLIFLIDQKKYDDAKACANASIARLKNLNRRTVDVLASRLYFYYSYVHELTNSLAEIR
Sbjct: 121 LLVLIFLIDQKKYDDAKACANASIARLKNLNRRTVDVLASRLYFYYSYVHELTNSLAEIR 180

Query: 181 GNLLALHRMATLHRDELGQETXXXXXXXXXXXXXXXDQAEKLRSKAPRFEAHSNQQFCRY 240
           GNLLALHRMATLHRDELGQET               DQAEKLRSKAPRFEAHSNQQFCRY
Sbjct: 181 GNLLALHRMATLHRDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQFCRY 240

Query: 241 LFYLGKIRTIQLEYTDAKESLLQAARKAPITARGFRIQCNKWAIIVRLLLGEIPERTVFM 300
           LFYLGKIRTIQLEYTDAKESLLQAARKAPITARGFRIQCNKWAIIVRLLLGEIPERTVFM
Sbjct: 241 LFYLGKIRTIQLEYTDAKESLLQAARKAPITARGFRIQCNKWAIIVRLLLGEIPERTVFM 300

Query: 301 QKGMKEALTPYFELTNAVRIGDLELFRAVADKFVSTFSADRTRNLIVRLRHNVIRTGLRN 360
           QKGMKEALTPYFELTNAVRIGDLELFRAVADKFVSTFSADRTRNLIVRLRHNVIRTGLRN
Sbjct: 301 QKGMKEALTPYFELTNAVRIGDLELFRAVADKFVSTFSADRTRNLIVRLRHNVIRTGLRN 360

Query: 361 ISISYSRISLADIAKKLRLDSDNPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDV 420
           ISISYSRISLADIAKKLRLDSDNPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDV
Sbjct: 361 ISISYSRISLADIAKKLRLDSDNPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDV 420

Query: 421 YSTNEPQIAFNSRIAFCLNMHNEAVKAMRFPPNSHXXXXXXXXXXXXXXXXXXXXXHMAE 480
           YSTNEPQIAFNSRIAFCLNMHNEAVKAMRFPPNSH                     HMAE
Sbjct: 421 YSTNEPQIAFNSRIAFCLNMHNEAVKAMRFPPNSHKEKESAEKRRERLQQEEELAKHMAE 480

Query: 481 EDDDDF 486
           EDDDDF
Sbjct: 481 EDDDDF 486



>Os09g0541900 Similar to 26S proteasome subunit RPN3a
          Length = 487

 Score =  775 bits (2002), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 381/484 (78%), Positives = 402/484 (83%)

Query: 3   DVQMSDSEXXXXXXXXXXXXXLHHLKEIASVIEAGSLSKEVRRISXXXXXXXXXXXXXXX 62
           DV+M+DS+             L HLKEIASVIEAGSL+KEVRRIS               
Sbjct: 4   DVEMNDSQPQPAAAPPPAASTLQHLKEIASVIEAGSLTKEVRRISRAVRLTVALRRRLAA 63

Query: 63  XXXXXFLAFALPPSSEAYTRLTSLVPKEDDTEMDIDMAAPATQISIKHGLPEIEIYCYLL 122
                FLAFALP SSEA+ RL+SL+PKED +EMD+D+AAPA Q+SIKHGLPEIEIYCYLL
Sbjct: 64  RDVAAFLAFALPHSSEAFARLSSLLPKEDGSEMDVDLAAPAAQVSIKHGLPEIEIYCYLL 123

Query: 123 VLIFLIDQKKYDDAKACANASIARLKNLNRRTVDVLASRLYFYYSYVHELTNSLAEIRGN 182
           VLIFLID KKYD+AKACA+ASI RLKNLNRRTVDVLASR++ YYSYVHELT+SLAEIRG 
Sbjct: 124 VLIFLIDNKKYDEAKACASASITRLKNLNRRTVDVLASRVFTYYSYVHELTSSLAEIRGT 183

Query: 183 LLALHRMATLHRDELGQETXXXXXXXXXXXXXXXDQAEKLRSKAPRFEAHSNQQFCRYLF 242
           LLALHRMATLHRDELGQET               DQAEKLRSKAPRFEAHSNQQFCRYLF
Sbjct: 184 LLALHRMATLHRDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQFCRYLF 243

Query: 243 YLGKIRTIQLEYTDAKESLLQAARKAPITARGFRIQCNKWAIIVRLLLGEIPERTVFMQK 302
           YLGKIRTIQLEYTDAKESLLQAARKAP TARGFR+QCNKWAIIVRLLLGEIPERTVFMQK
Sbjct: 244 YLGKIRTIQLEYTDAKESLLQAARKAPTTARGFRVQCNKWAIIVRLLLGEIPERTVFMQK 303

Query: 303 GMKEALTPYFELTNAVRIGDLELFRAVADKFVSTFSADRTRNLIVRLRHNVIRTGLRNIS 362
           GMK AL PYFELTNAVR+GDLELFRAVA+KF STFSADRTRNLIVRLRHNVIRTGLRNIS
Sbjct: 304 GMKAALAPYFELTNAVRVGDLELFRAVAEKFASTFSADRTRNLIVRLRHNVIRTGLRNIS 363

Query: 363 ISYSRISLADIAKKLRLDSDNPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYS 422
           ISYSRISLADIAKKLRLDS+NPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYS
Sbjct: 364 ISYSRISLADIAKKLRLDSENPVADAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYS 423

Query: 423 TNEPQIAFNSRIAFCLNMHNEAVKAMRFPPNSHXXXXXXXXXXXXXXXXXXXXXHMAEED 482
           TNEPQIAFNSRIAFCLNMHNEAVKA+RFPPNSH                     HMAEED
Sbjct: 424 TNEPQIAFNSRIAFCLNMHNEAVKALRFPPNSHKEKESAEKRRERLQQEEELAKHMAEED 483

Query: 483 DDDF 486
           DDDF
Sbjct: 484 DDDF 487



>AK108887 
          Length = 648

 Score =  293 bits (749), Expect = 2e-79,   Method: Compositional matrix adjust.
 Identities = 166/370 (44%), Positives = 211/370 (57%), Gaps = 27/370 (7%)

Query: 112 LPEIEIYCYLLVLIFLIDQKKYDDAKACANASIARLKNLNRRTVDVLASRLYFYYSYVHE 171
           LPEI+ Y +LLV+I+LID    ++A A A A    L   NRR++D L+++  +YY   HE
Sbjct: 253 LPEIQTYLHLLVIIYLIDHSAKEEAVAAATALNDFLAKHNRRSLDALSAKALYYYVLTHE 312

Query: 172 LTNSLAEIRGNLLALHRMATLHRDELGQETXXXXXXXXXXXXXXXDQAEKLRSKAPRFEA 231
                  IR NLL  HR A L  DE GQ                  QA+K R  +  F  
Sbjct: 313 KVGRANAIRNNLLLAHRTACLRHDEPGQAVLTNAILRNYLQNNLYTQADKFRLNS-HFPT 371

Query: 232 H-SNQQFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIT-ARGFRIQCNKWAIIVRLL 289
           H SN Q  RYL+Y+GKI+T+QL Y+DA  SL Q+ RKAP   A GFR    K  +IV+LL
Sbjct: 372 HISNSQHARYLYYIGKIQTVQLHYSDAYTSLSQSIRKAPTAGAIGFRQTVTKLLVIVQLL 431

Query: 290 LGEIPERTVFMQKGMKEALTPYFELTNAVRIGDLELFRAVADKFVSTFSADRTRNLIVRL 349
           +G+IPER++F    +K +L PYF+LT AVR+G+L  F        + F  D     I RL
Sbjct: 432 MGDIPERSLFRTPQLKRSLKPYFQLTQAVRVGNLAAFGTCVASHEAIFRRDHMLTFIARL 491

Query: 350 RHNVIRTGLRNISISYSRISLADIAKKLRLDSDNPVA----------------------- 386
           RHNVI+TGLR I+ISYSRIS+ DI  KL L      A                       
Sbjct: 492 RHNVIKTGLRRINISYSRISIHDICDKLHLTEATAHAGSPHHAASVGAAAAAGISVGSSE 551

Query: 387 -DAESIVAKAIRDGAIDATIDHANGWMVSKETGDVYSTNEPQIAFNSRIAFCLNMHNEAV 445
            DAE IVAKAI DG I+ATID    ++ SK+  DVY+TNEPQ  F+ RI FCL+ +N AV
Sbjct: 552 EDAEFIVAKAIHDGVIEATIDRKQHFVFSKDNADVYATNEPQQQFHKRIQFCLDSYNAAV 611

Query: 446 KAMRFPPNSH 455
           K MRFPP++H
Sbjct: 612 KDMRFPPDAH 621



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,279,539
Number of extensions: 409517
Number of successful extensions: 992
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 985
Number of HSP's successfully gapped: 3
Length of query: 486
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 381
Effective length of database: 11,553,331
Effective search space: 4401819111
Effective search space used: 4401819111
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 158 (65.5 bits)