BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0548400 Os08g0548400|Os08g0548400
         (146 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0548400  Similar to Chaperone protein dnaJ                   160   3e-40
Os03g0244950  Similar to Chaperone protein dnaJ                    89   1e-18
Os06g0716100  Similar to Chaperone protein dnaJ                    88   2e-18
Os01g0101700  Heat shock protein DnaJ, N-terminal domain con...    68   3e-12

>Os08g0548400 Similar to Chaperone protein dnaJ
          Length = 146

 Score =  160 bits (405), Expect = 3e-40,   Method: Compositional matrix adjust.
 Identities = 92/146 (63%), Positives = 92/146 (63%)

Query: 1   MISAPSVGIXXXXXXXXXXXVGSYXXXXXXXXXXXXXXXXXXGTLYDVLGLRAGATVREI 60
           MISAPSVGI           VGSY                  GTLYDVLGLRAGATVREI
Sbjct: 1   MISAPSVGIAFAPKPPPAAAVGSYRARKVRCAVAVAPAPAPAGTLYDVLGLRAGATVREI 60

Query: 61  KAAYRRLARERHPDVAASAGADDFVRLHDAYATLSDPDSRARYDRDVVAVASMARXXXXX 120
           KAAYRRLARERHPDVAASAGADDFVRLHDAYATLSDPDSRARYDRDVVAVASMAR     
Sbjct: 61  KAAYRRLARERHPDVAASAGADDFVRLHDAYATLSDPDSRARYDRDVVAVASMARGAHHR 120

Query: 121 XXXXXXXXXXXXXXXXXXXXETDQCW 146
                               ETDQCW
Sbjct: 121 TMAAPAAAPRWYGRRPRRTWETDQCW 146



>Os03g0244950 Similar to Chaperone protein dnaJ
          Length = 165

 Score = 88.6 bits (218), Expect = 1e-18,   Method: Compositional matrix adjust.
 Identities = 51/106 (48%), Positives = 63/106 (59%), Gaps = 8/106 (7%)

Query: 44  TLYDVLGLRAGATVREIKAAYRRLARERHPDVA---ASAGADDFVRLHDAYATLSDPDSR 100
           + YDVLGL AGA+ REIK AYRRLAR  HPD +   A+A +DDF+R+H AY+TLSDPD R
Sbjct: 65  SFYDVLGLHAGASAREIKDAYRRLARAVHPDASPHPAAASSDDFIRVHAAYSTLSDPDKR 124

Query: 101 ARYDRDVVAVASMARXXXXXXXXXXXXXXXXXXXXXXXXXETDQCW 146
           A YDR ++ +A+  R                         ETDQCW
Sbjct: 125 ADYDRRLLLLAAGRR-----RATPSLGRSPTFPARRRRTWETDQCW 165



>Os06g0716100 Similar to Chaperone protein dnaJ
          Length = 133

 Score = 88.2 bits (217), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 52/103 (50%), Positives = 61/103 (59%), Gaps = 13/103 (12%)

Query: 44  TLYDVLGLRAGATVREIKAAYRRLARERHPDVAASAGADDFVRLHDAYATLSDPDSRARY 103
           T Y+VLG+ AGA+  EIKAAYRRLARE HPD  A+ G +DF+RLH AYATL+DPD RARY
Sbjct: 44  THYEVLGVGAGASRGEIKAAYRRLAREVHPDAGAT-GDEDFIRLHAAYATLADPDERARY 102

Query: 104 DRDVVAVASMARXXXXXXXXXXXXXXXXXXXXXXXXXETDQCW 146
           DR +   A+ A                          ETDQCW
Sbjct: 103 DRAMAGPAASA------------FRRAPASSFRRRTWETDQCW 133



>Os01g0101700 Heat shock protein DnaJ, N-terminal domain containing protein
          Length = 170

 Score = 67.8 bits (164), Expect = 3e-12,   Method: Compositional matrix adjust.
 Identities = 34/66 (51%), Positives = 46/66 (69%), Gaps = 4/66 (6%)

Query: 44  TLYDVLGLRAGATVREIKAAYRRLARERHPDV----AASAGADDFVRLHDAYATLSDPDS 99
           TLYD+LG+ +  T+ E++AAYRR+AR+ HPDV    AA+     F+ + +AY TLSDP  
Sbjct: 54  TLYDLLGISSEGTLDEVRAAYRRMARKYHPDVSPPDAAAENTRRFIEVQEAYETLSDPSR 113

Query: 100 RARYDR 105
           RA YDR
Sbjct: 114 RATYDR 119



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,810,580
Number of extensions: 80796
Number of successful extensions: 507
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 534
Number of HSP's successfully gapped: 4
Length of query: 146
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 55
Effective length of database: 12,284,327
Effective search space: 675637985
Effective search space used: 675637985
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 151 (62.8 bits)