BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0542300 Os08g0542300|J065019N01
(54 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0542300 Similar to 60S ribosomal protein L7-2 116 5e-27
Os08g0542100 Ribosomal protein L7, eukaryotic form family p... 87 2e-18
Os04g0605900 Similar to 60S ribosomal protein L7-2 67 2e-12
Os08g0234000 Similar to 60S ribosomal protein L7-2 65 1e-11
>Os08g0542300 Similar to 60S ribosomal protein L7-2
Length = 54
Score = 116 bits (290), Expect = 5e-27, Method: Compositional matrix adjust.
Identities = 54/54 (100%), Positives = 54/54 (100%)
Query: 1 SWLTSSDLFKKALGEHGIICLEDEHEIATVGPHFRQASNFLMPFKLKCQEGGYR 54
SWLTSSDLFKKALGEHGIICLEDEHEIATVGPHFRQASNFLMPFKLKCQEGGYR
Sbjct: 1 SWLTSSDLFKKALGEHGIICLEDEHEIATVGPHFRQASNFLMPFKLKCQEGGYR 54
>Os08g0542100 Ribosomal protein L7, eukaryotic form family protein
Length = 248
Score = 87.4 bits (215), Expect = 2e-18, Method: Composition-based stats.
Identities = 40/49 (81%), Positives = 45/49 (91%), Gaps = 1/49 (2%)
Query: 3 LTSSDLFKKALGEHGIICLED-EHEIATVGPHFRQASNFLMPFKLKCQE 50
LTS+DL +KALGE+GIICLED HEIA+VGPHFR+ASNFLMPFKLKC E
Sbjct: 169 LTSNDLIEKALGEYGIICLEDLVHEIASVGPHFREASNFLMPFKLKCPE 217
>Os04g0605900 Similar to 60S ribosomal protein L7-2
Length = 244
Score = 67.4 bits (163), Expect = 2e-12, Method: Composition-based stats.
Identities = 30/53 (56%), Positives = 41/53 (77%), Gaps = 1/53 (1%)
Query: 3 LTSSDLFKKALGEHGIICLED-EHEIATVGPHFRQASNFLMPFKLKCQEGGYR 54
LT++ + ++ LG+H IIC+ED HEI TVGPHF++A+NFL PFKLK GG +
Sbjct: 165 LTNNKVIEEGLGKHDIICIEDLVHEIMTVGPHFKEANNFLWPFKLKAPLGGLK 217
>Os08g0234000 Similar to 60S ribosomal protein L7-2
Length = 245
Score = 65.1 bits (157), Expect = 1e-11, Method: Composition-based stats.
Identities = 29/53 (54%), Positives = 40/53 (75%), Gaps = 1/53 (1%)
Query: 3 LTSSDLFKKALGEHGIICLED-EHEIATVGPHFRQASNFLMPFKLKCQEGGYR 54
L ++ + ++ LG+H IIC+ED HEI TVGPHF++A+NFL PFKLK GG +
Sbjct: 166 LQNNKVIEEGLGKHDIICIEDLVHEIMTVGPHFKEANNFLWPFKLKAPLGGLK 218
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.322 0.139 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,956,981
Number of extensions: 51431
Number of successful extensions: 59
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 4
Length of query: 54
Length of database: 17,035,801
Length adjustment: 27
Effective length of query: 27
Effective length of database: 15,626,023
Effective search space: 421902621
Effective search space used: 421902621
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)