BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0538000 Os08g0538000|AK119836
         (698 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0538000  Similar to Glycyl-tRNA synthetase, mitochondri...  1293   0.0  
Os04g0398300  Similar to Glycyl-tRNA synthetase, mitochondri...   465   e-131
AK059027                                                          283   4e-76

>Os08g0538000 Similar to Glycyl-tRNA synthetase, mitochondrial precursor (EC
           6.1.1.14) (Glycine--tRNA ligase) (GlyRS)
          Length = 698

 Score = 1293 bits (3345), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 618/618 (100%), Positives = 618/618 (100%)

Query: 81  REEMRQAVVNTLERKLFYIPSFKIYRGVAGLYDYGPPGCAVKANVLAFWRQHFVLEENML 140
           REEMRQAVVNTLERKLFYIPSFKIYRGVAGLYDYGPPGCAVKANVLAFWRQHFVLEENML
Sbjct: 81  REEMRQAVVNTLERKLFYIPSFKIYRGVAGLYDYGPPGCAVKANVLAFWRQHFVLEENML 140

Query: 141 EVDCPCVTPEVVLKASGHVEKFTDLMVKDEKTGTCYRADHLLKDHCKEKLEKDLTLSPEK 200
           EVDCPCVTPEVVLKASGHVEKFTDLMVKDEKTGTCYRADHLLKDHCKEKLEKDLTLSPEK
Sbjct: 141 EVDCPCVTPEVVLKASGHVEKFTDLMVKDEKTGTCYRADHLLKDHCKEKLEKDLTLSPEK 200

Query: 201 AAELKHVLAVLDDLSADELGAKIKEYGIVAPDTKNPLSDPYPFNLMFQTSIGPTGLNVGY 260
           AAELKHVLAVLDDLSADELGAKIKEYGIVAPDTKNPLSDPYPFNLMFQTSIGPTGLNVGY
Sbjct: 201 AAELKHVLAVLDDLSADELGAKIKEYGIVAPDTKNPLSDPYPFNLMFQTSIGPTGLNVGY 260

Query: 261 MRPETAQGIFVNFKDLYYYNGQKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFV 320
           MRPETAQGIFVNFKDLYYYNGQKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFV
Sbjct: 261 MRPETAQGIFVNFKDLYYYNGQKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFV 320

Query: 321 DPEDKSHPKFVDVADLEFLMFPRELQLSGESAKLVKLGEAVSKGTVNNETLGYFIGRVYL 380
           DPEDKSHPKFVDVADLEFLMFPRELQLSGESAKLVKLGEAVSKGTVNNETLGYFIGRVYL
Sbjct: 321 DPEDKSHPKFVDVADLEFLMFPRELQLSGESAKLVKLGEAVSKGTVNNETLGYFIGRVYL 380

Query: 381 FLTRLGIDKNRLRFRQHLPNEMAHYAADCWDAEIECSYGWIECVGIADRSAYDLRAHSDK 440
           FLTRLGIDKNRLRFRQHLPNEMAHYAADCWDAEIECSYGWIECVGIADRSAYDLRAHSDK
Sbjct: 381 FLTRLGIDKNRLRFRQHLPNEMAHYAADCWDAEIECSYGWIECVGIADRSAYDLRAHSDK 440

Query: 441 SGVPLVAHEKFSKPREVEKLVIVPSKKDLGLAFKGNQKMVVEALEAMSEKEAMDMKAALE 500
           SGVPLVAHEKFSKPREVEKLVIVPSKKDLGLAFKGNQKMVVEALEAMSEKEAMDMKAALE
Sbjct: 441 SGVPLVAHEKFSKPREVEKLVIVPSKKDLGLAFKGNQKMVVEALEAMSEKEAMDMKAALE 500

Query: 501 SKGETNFQVCTLGKDVVITKKMVSISMEKKLEHQRVFTPSVIEPSFGIGRIIYCLFEHSF 560
           SKGETNFQVCTLGKDVVITKKMVSISMEKKLEHQRVFTPSVIEPSFGIGRIIYCLFEHSF
Sbjct: 501 SKGETNFQVCTLGKDVVITKKMVSISMEKKLEHQRVFTPSVIEPSFGIGRIIYCLFEHSF 560

Query: 561 YTRPSKSEEEQLNVFRFPPIVAPIKCTVFPLVKNQEFDDAAKVIDKALTTAGISHIIDTT 620
           YTRPSKSEEEQLNVFRFPPIVAPIKCTVFPLVKNQEFDDAAKVIDKALTTAGISHIIDTT
Sbjct: 561 YTRPSKSEEEQLNVFRFPPIVAPIKCTVFPLVKNQEFDDAAKVIDKALTTAGISHIIDTT 620

Query: 621 AISIGRRYARTDEIGVPFAVTVDSATSVTIRERDSKEQIRVGIDEVASVVKQLTDGQSTW 680
           AISIGRRYARTDEIGVPFAVTVDSATSVTIRERDSKEQIRVGIDEVASVVKQLTDGQSTW
Sbjct: 621 AISIGRRYARTDEIGVPFAVTVDSATSVTIRERDSKEQIRVGIDEVASVVKQLTDGQSTW 680

Query: 681 ADVSFKYPSHIGPQGDQE 698
           ADVSFKYPSHIGPQGDQE
Sbjct: 681 ADVSFKYPSHIGPQGDQE 698



>Os04g0398300 Similar to Glycyl-tRNA synthetase, mitochondrial precursor (EC
           6.1.1.14) (Glycine--tRNA ligase) (GlyRS)
          Length = 292

 Score =  465 bits (1197), Expect = e-131,   Method: Compositional matrix adjust.
 Identities = 220/279 (78%), Positives = 251/279 (89%)

Query: 412 AEIECSYGWIECVGIADRSAYDLRAHSDKSGVPLVAHEKFSKPREVEKLVIVPSKKDLGL 471
           AEIECS+GWIECVGIADRSAYDLRAHSDKSGV L AHEKF++PREVEKLVI PSKK+LGL
Sbjct: 1   AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPREVEKLVITPSKKELGL 60

Query: 472 AFKGNQKMVVEALEAMSEKEAMDMKAALESKGETNFQVCTLGKDVVITKKMVSISMEKKL 531
           AFKGNQ+MV+EALEAMSE EA++MK+ALESKGE  F+VCTLGKDV I K MVSI+MEKK 
Sbjct: 61  AFKGNQRMVLEALEAMSETEALNMKSALESKGEVEFKVCTLGKDVTIKKSMVSINMEKKK 120

Query: 532 EHQRVFTPSVIEPSFGIGRIIYCLFEHSFYTRPSKSEEEQLNVFRFPPIVAPIKCTVFPL 591
           EHQR FTPSVIEPSFGIGRIIYCLFEH FY RP K+E+EQLNVF FPP+VAPIKCTVFPL
Sbjct: 121 EHQRKFTPSVIEPSFGIGRIIYCLFEHCFYQRPGKAEDEQLNVFGFPPLVAPIKCTVFPL 180

Query: 592 VKNQEFDDAAKVIDKALTTAGISHIIDTTAISIGRRYARTDEIGVPFAVTVDSATSVTIR 651
           VK ++F+  AK I KALT AGISHIID T  +IG+RYARTDEIGVP A+TVD+ TSVT+R
Sbjct: 181 VKIEKFEVVAKKISKALTAAGISHIIDMTGNTIGKRYARTDEIGVPLAITVDNTTSVTVR 240

Query: 652 ERDSKEQIRVGIDEVASVVKQLTDGQSTWADVSFKYPSH 690
           +RDSK+QIRV +DEVASVVK++TDGQSTWAD+ ++YP+H
Sbjct: 241 DRDSKDQIRVEVDEVASVVKEVTDGQSTWADIMWRYPAH 279



>AK059027 
          Length = 179

 Score =  283 bits (723), Expect = 4e-76,   Method: Compositional matrix adjust.
 Identities = 130/151 (86%), Positives = 142/151 (94%)

Query: 307 RVREFTLAEIEHFVDPEDKSHPKFVDVADLEFLMFPRELQLSGESAKLVKLGEAVSKGTV 366
           RVREFTLAEIEHFVDPEDKSHPKF DV+DLEF MFPRE QL+G+SA  + +G+AVS+GTV
Sbjct: 1   RVREFTLAEIEHFVDPEDKSHPKFRDVSDLEFFMFPREQQLTGKSAMKLNIGQAVSEGTV 60

Query: 367 NNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLPNEMAHYAADCWDAEIECSYGWIECVGI 426
           NNETLGYFIGRVYLFLT+LGIDK+RLRFRQHLPNEMAHYAADCWDAEIECS+GWIECVGI
Sbjct: 61  NNETLGYFIGRVYLFLTQLGIDKDRLRFRQHLPNEMAHYAADCWDAEIECSFGWIECVGI 120

Query: 427 ADRSAYDLRAHSDKSGVPLVAHEKFSKPREV 457
           ADRSAYDLRAHSDKSGV L AHEKF++PRE 
Sbjct: 121 ADRSAYDLRAHSDKSGVALEAHEKFAEPREA 151



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 20,481,030
Number of extensions: 843484
Number of successful extensions: 1891
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1891
Number of HSP's successfully gapped: 3
Length of query: 698
Length of database: 17,035,801
Length adjustment: 108
Effective length of query: 590
Effective length of database: 11,396,689
Effective search space: 6724046510
Effective search space used: 6724046510
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 160 (66.2 bits)