BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0524400 Os08g0524400|J100068F10
         (263 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0524400  DOMON related domain containing protein             289   1e-78
Os08g0524200  DOMON related domain containing protein             111   6e-25

>Os08g0524400 DOMON related domain containing protein
          Length = 263

 Score =  289 bits (740), Expect = 1e-78,   Method: Compositional matrix adjust.
 Identities = 152/193 (78%), Positives = 152/193 (78%)

Query: 1   MAHPHRXXXXXXXXXXXXXXXXXXXXXXXXGEKFPAGRAYAACEDLPSLGAALHYTYDAS 60
           MAHPHR                        GEKFPAGRAYAACEDLPSLGAALHYTYDAS
Sbjct: 1   MAHPHRLVAVLALILLASPAAMRAAEAACAGEKFPAGRAYAACEDLPSLGAALHYTYDAS 60

Query: 61  KSSLSXXXXXXXXXXXXXXXXXLNPTGEGMAGTQALVALKGGSSSSAPAVKTYNITGYVA 120
           KSSLS                 LNPTGEGMAGTQALVALKGGSSSSAPAVKTYNITGYVA
Sbjct: 61  KSSLSVAFVAAPAGAGGWVAWGLNPTGEGMAGTQALVALKGGSSSSAPAVKTYNITGYVA 120

Query: 121 LGGASTPIAFPATDLAADEGSGGKIRLYGKLQLHKGMKSVNQVWQVGSSVTGGAPDKHAF 180
           LGGASTPIAFPATDLAADEGSGGKIRLYGKLQLHKGMKSVNQVWQVGSSVTGGAPDKHAF
Sbjct: 121 LGGASTPIAFPATDLAADEGSGGKIRLYGKLQLHKGMKSVNQVWQVGSSVTGGAPDKHAF 180

Query: 181 GPANLASKAKLVL 193
           GPANLASKAKLVL
Sbjct: 181 GPANLASKAKLVL 193



>Os08g0524200 DOMON related domain containing protein
          Length = 390

 Score =  111 bits (277), Expect = 6e-25,   Method: Compositional matrix adjust.
 Identities = 74/164 (45%), Positives = 95/164 (57%), Gaps = 7/164 (4%)

Query: 32  EKFPAGRAYAACEDLPSLGAALHYTYDASKSSLSXXXXXXXXXX-XXXXXXXLNPTGEGM 90
           E+F + R YAAC DLP LGA++H+TYDA+ S+                    LNPTG GM
Sbjct: 29  ERFSSNRVYAACSDLPHLGASVHWTYDAAASASLSVAFVAAPPSPGGWVAWGLNPTGGGM 88

Query: 91  AGTQALVAL-KGGSSSSAPAVKTYNITGYVALGGASTPIAFPATDLAADEGSGGKIRLYG 149
           AGTQALVAL KGG       V+T++I GY     A   + +PATDLAA+  + G++ ++G
Sbjct: 89  AGTQALVALPKGGGGGYE--VQTFDIEGYSL--SAPGKLKYPATDLAAEVAADGRVSVFG 144

Query: 150 KLQLHKGMKSVNQVWQVGSSVTGGAPDKHAFGPANLASKAKLVL 193
           KL L  G   VNQVWQVG  V+ G+   HA    N A+  KL L
Sbjct: 145 KLALQNGTAEVNQVWQVG-PVSSGSMVPHAMSSDNKAAMGKLNL 187



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.129    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,957,855
Number of extensions: 228758
Number of successful extensions: 703
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 698
Number of HSP's successfully gapped: 4
Length of query: 263
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 164
Effective length of database: 11,866,615
Effective search space: 1946124860
Effective search space used: 1946124860
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 155 (64.3 bits)