BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0505300 Os08g0505300|D29722
         (66 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0505300  Similar to 60S ribosomal protein L31                 96   5e-21
Os06g0319700  Similar to 60S ribosomal protein L31                 87   3e-18
Os02g0717800  Similar to 60S ribosomal protein L31                 79   5e-16

>Os08g0505300 Similar to 60S ribosomal protein L31
          Length = 66

 Score = 95.9 bits (237), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 51/66 (77%), Positives = 51/66 (77%)

Query: 1  RVDVKLNKHIWSSGIXXXXXXXXXXXXXXXNDEEDAKEELYSLVTVAEVPPEGLKGLGTK 60
          RVDVKLNKHIWSSGI               NDEEDAKEELYSLVTVAEVPPEGLKGLGTK
Sbjct: 1  RVDVKLNKHIWSSGIRSVPRRVRVRIARKRNDEEDAKEELYSLVTVAEVPPEGLKGLGTK 60

Query: 61 VVDETD 66
          VVDETD
Sbjct: 61 VVDETD 66



>Os06g0319700 Similar to 60S ribosomal protein L31
          Length = 124

 Score = 87.0 bits (214), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 45/64 (70%), Positives = 48/64 (75%)

Query: 1   RVDVKLNKHIWSSGIXXXXXXXXXXXXXXXNDEEDAKEELYSLVTVAEVPPEGLKGLGTK 60
           RVDVKLNKHIWSSGI               NDEEDAKEELYSLVTVAEVP EGLKGLGTK
Sbjct: 59  RVDVKLNKHIWSSGIRSVPRRVRVRIARRRNDEEDAKEELYSLVTVAEVPQEGLKGLGTK 118

Query: 61  VVDE 64
           +V++
Sbjct: 119 LVED 122



>Os02g0717800 Similar to 60S ribosomal protein L31
          Length = 98

 Score = 79.3 bits (194), Expect = 5e-16,   Method: Compositional matrix adjust.
 Identities = 41/64 (64%), Positives = 47/64 (73%)

Query: 1  RVDVKLNKHIWSSGIXXXXXXXXXXXXXXXNDEEDAKEELYSLVTVAEVPPEGLKGLGTK 60
          R+DVKLNK IW++GI               NDEEDAKEELYSLVTVAE+P EGLKGLGTK
Sbjct: 33 RIDVKLNKAIWTNGIRSVPRRVRVRISRKRNDEEDAKEELYSLVTVAEIPAEGLKGLGTK 92

Query: 61 VVDE 64
          VV++
Sbjct: 93 VVED 96



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.306    0.131    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,832,592
Number of extensions: 41063
Number of successful extensions: 29
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 3
Length of query: 66
Length of database: 17,035,801
Length adjustment: 38
Effective length of query: 28
Effective length of database: 15,051,669
Effective search space: 421446732
Effective search space used: 421446732
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)
S2: 149 (62.0 bits)