BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0486700 Os08g0486700|J065121F09
         (164 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0486700  Conserved hypothetical protein                      173   5e-44
Os09g0473600  Conserved hypothetical protein                       70   7e-13

>Os08g0486700 Conserved hypothetical protein
          Length = 164

 Score =  173 bits (438), Expect = 5e-44,   Method: Compositional matrix adjust.
 Identities = 94/164 (57%), Positives = 94/164 (57%)

Query: 1   MESSHITGDDGEGCNSSESGWTMYLASPMHXXXXXXXXXXXXXXXNVDDGYGYISGRGSR 60
           MESSHITGDDGEGCNSSESGWTMYLASPMH               NVDDGYGYISGRGSR
Sbjct: 1   MESSHITGDDGEGCNSSESGWTMYLASPMHGDDDGGGKRSGSEGSNVDDGYGYISGRGSR 60

Query: 61  KEYEXXXXXXXXXSLASDASTGPAKVKVPSAPDGDDAXXXXXXXXXXXXXXXXXXXXXXX 120
           KEYE         SLASDASTGPAKVKVPSAPDGDDA                       
Sbjct: 61  KEYEDDGDGDDDDSLASDASTGPAKVKVPSAPDGDDAGGRRKHDGDEDGGGKGEEEEEED 120

Query: 121 XXLHTKFSMXXXXXXXXXXXXXXXXXXXXXQNKRGSSSRTRFFW 164
             LHTKFSM                     QNKRGSSSRTRFFW
Sbjct: 121 HGLHTKFSMSSGKKAGKTEKGGEGKSSKKGQNKRGSSSRTRFFW 164



>Os09g0473600 Conserved hypothetical protein
          Length = 165

 Score = 70.1 bits (170), Expect = 7e-13,   Method: Compositional matrix adjust.
 Identities = 44/97 (45%), Positives = 53/97 (54%), Gaps = 4/97 (4%)

Query: 1  MESSHITGDDGEGCNSSESGWTMYLASPMHXXXXXXXXXXXXXXXNVDDGYGYISGRGSR 60
          MES  +  DDGE CNS+ESGWTMYLASP H               NV+DG G+ + R   
Sbjct: 1  MESPRLIEDDGEECNSNESGWTMYLASPTH---SDDVRAIVSEGSNVEDGSGFSNERRRG 57

Query: 61 KEYEXXXXXXXX-XSLASDASTGPAKVKVPSAPDGDD 96
          KE +          SLASDASTGPA+VK+    +  D
Sbjct: 58 KENKGHANDDGDYDSLASDASTGPAEVKMQEGKEEKD 94



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.309    0.127    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,749,553
Number of extensions: 99577
Number of successful extensions: 101
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 99
Number of HSP's successfully gapped: 2
Length of query: 164
Length of database: 17,035,801
Length adjustment: 93
Effective length of query: 71
Effective length of database: 12,179,899
Effective search space: 864772829
Effective search space used: 864772829
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 152 (63.2 bits)