BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0474600 Os08g0474600|AK101574
         (174 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0474600  Heat shock protein DnaJ, N-terminal domain con...   362   e-100
Os09g0460000  Heat shock protein DnaJ, N-terminal domain con...   230   4e-61

>Os08g0474600 Heat shock protein DnaJ, N-terminal domain containing protein
          Length = 174

 Score =  362 bits (928), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 174/174 (100%), Positives = 174/174 (100%)

Query: 1   MPLSSNISTQKTHYEVLSVNEGATYDEVRAGYRAAILNAHPDKSQAKLDSLVSSVEHGEF 60
           MPLSSNISTQKTHYEVLSVNEGATYDEVRAGYRAAILNAHPDKSQAKLDSLVSSVEHGEF
Sbjct: 1   MPLSSNISTQKTHYEVLSVNEGATYDEVRAGYRAAILNAHPDKSQAKLDSLVSSVEHGEF 60

Query: 61  FSVQKAWEVLRDPKSRTEYDKQLQTSRQNSANIAYEIEIEEMTVESTGDAMELMYPCRCG 120
           FSVQKAWEVLRDPKSRTEYDKQLQTSRQNSANIAYEIEIEEMTVESTGDAMELMYPCRCG
Sbjct: 61  FSVQKAWEVLRDPKSRTEYDKQLQTSRQNSANIAYEIEIEEMTVESTGDAMELMYPCRCG 120

Query: 121 DHFSILSSDLAEMGILVDDDGEIYFQSSDCVSASVVLSCGSCSLKTRLVINRTS 174
           DHFSILSSDLAEMGILVDDDGEIYFQSSDCVSASVVLSCGSCSLKTRLVINRTS
Sbjct: 121 DHFSILSSDLAEMGILVDDDGEIYFQSSDCVSASVVLSCGSCSLKTRLVINRTS 174



>Os09g0460000 Heat shock protein DnaJ, N-terminal domain containing protein
          Length = 173

 Score =  230 bits (586), Expect = 4e-61,   Method: Compositional matrix adjust.
 Identities = 108/168 (64%), Positives = 133/168 (79%)

Query: 6   NISTQKTHYEVLSVNEGATYDEVRAGYRAAILNAHPDKSQAKLDSLVSSVEHGEFFSVQK 65
           N S Q+T YEVLSV + ATYDE+RA Y++A+LN HPDK+Q  L+ LVSS E  EF SVQK
Sbjct: 6   NFSNQETLYEVLSVRKDATYDEIRAAYKSAVLNTHPDKAQMALNPLVSSSERNEFLSVQK 65

Query: 66  AWEVLRDPKSRTEYDKQLQTSRQNSANIAYEIEIEEMTVESTGDAMELMYPCRCGDHFSI 125
           AWE+LR PKSR EYDKQLQ+SRQN   +A EIEI++M VEST D++EL+YPCRCGD+FSI
Sbjct: 66  AWEILRYPKSRAEYDKQLQSSRQNLEIVATEIEIDDMIVESTADSVELLYPCRCGDYFSI 125

Query: 126 LSSDLAEMGILVDDDGEIYFQSSDCVSASVVLSCGSCSLKTRLVINRT 173
            S +L ++GI V +DGE+   +SD V +SVVL CGSCSLK RLV N+T
Sbjct: 126 TSRELGQIGISVREDGEMELHTSDSVPSSVVLGCGSCSLKARLVTNKT 173



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.127    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,259,094
Number of extensions: 180878
Number of successful extensions: 577
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 594
Number of HSP's successfully gapped: 2
Length of query: 174
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 80
Effective length of database: 12,127,685
Effective search space: 970214800
Effective search space used: 970214800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 152 (63.2 bits)