BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0459300 Os08g0459300|AK060409
         (226 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0459300  Conserved hypothetical protein                      397   e-111
Os02g0834300  Conserved hypothetical protein                      110   7e-25

>Os08g0459300 Conserved hypothetical protein
          Length = 226

 Score =  397 bits (1021), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 197/226 (87%), Positives = 197/226 (87%)

Query: 1   MAATTTIPARPXXXXXXXXXXXXXXXXXDSKNNPADQLVALINSNRTASKASTLDDNQGL 60
           MAATTTIPARP                 DSKNNPADQLVALINSNRTASKASTLDDNQGL
Sbjct: 1   MAATTTIPARPLLAAVLAALLLSAASAADSKNNPADQLVALINSNRTASKASTLDDNQGL 60

Query: 61  GCIALQYIKAYEGQCNQVGESKKPPETSFAETFAPNCGVQAATLTKITGRLLACQSNYAT 120
           GCIALQYIKAYEGQCNQVGESKKPPETSFAETFAPNCGVQAATLTKITGRLLACQSNYAT
Sbjct: 61  GCIALQYIKAYEGQCNQVGESKKPPETSFAETFAPNCGVQAATLTKITGRLLACQSNYAT 120

Query: 121 PDQAFNFLVNDAKSIQVLHSKNHTEXXXXXXXXXXXXPYFWCVLFSSGKPTTSFKVDGGV 180
           PDQAFNFLVNDAKSIQVLHSKNHTE            PYFWCVLFSSGKPTTSFKVDGGV
Sbjct: 121 PDQAFNFLVNDAKSIQVLHSKNHTEVGAAVSGTSGGGPYFWCVLFSSGKPTTSFKVDGGV 180

Query: 181 PKSVRPGCFSGNNDDCMGANAAVSIGAGTWRLVAALLFSAACVFAL 226
           PKSVRPGCFSGNNDDCMGANAAVSIGAGTWRLVAALLFSAACVFAL
Sbjct: 181 PKSVRPGCFSGNNDDCMGANAAVSIGAGTWRLVAALLFSAACVFAL 226



>Os02g0834300 Conserved hypothetical protein
          Length = 230

 Score =  110 bits (275), Expect = 7e-25,   Method: Compositional matrix adjust.
 Identities = 65/175 (37%), Positives = 91/175 (52%), Gaps = 21/175 (12%)

Query: 31  KNNPADQLVALINSNRTASKASTLDDNQGLGCIALQYIKAYEGQCNQVG--------ESK 82
             NPA+ LVAL+N+NRTA+K   L  + GLGC+ALQYI      C  +G         + 
Sbjct: 26  HGNPANDLVALVNANRTATKLPHLRTSAGLGCMALQYIS----DCIGIGIGCAGDNTVAC 81

Query: 83  KPPETSFAETFAPNCGVQAATLTKITGRLLACQSNYATPDQAFNFLV----NDAKSIQVL 138
           +PPE    E +A NCGV+  T+  ITGRLL C    +  + A   ++    N   +  V+
Sbjct: 82  QPPEAHITEVYAANCGVELPTVDVITGRLLGCHRQRSDAEAALEAVLSGSGNSTAARAVI 141

Query: 139 HSKNHTEXXXXXXXXXXXXPYFWCVLFSSGKPTTSFKVDG---GVPKSVRPGCFS 190
             K HT+            P+FWC+LFSSG   ++F ++    GV +S   GCFS
Sbjct: 142 RGKEHTQVGAGFDRAHRRGPFFWCLLFSSGSANSTFLLEAAGKGVHQS--HGCFS 194



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.130    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,663,324
Number of extensions: 244614
Number of successful extensions: 420
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 417
Number of HSP's successfully gapped: 2
Length of query: 226
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 129
Effective length of database: 11,971,043
Effective search space: 1544264547
Effective search space used: 1544264547
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 154 (63.9 bits)