BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0442300 Os08g0442300|AK071719
         (113 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0442300  Similar to Calcineurin-like protein                 237   1e-63
Os02g0802400  EF-Hand type domain containing protein              161   1e-40

>Os08g0442300 Similar to Calcineurin-like protein
          Length = 113

 Score =  237 bits (605), Expect = 1e-63,   Method: Compositional matrix adjust.
 Identities = 113/113 (100%), Positives = 113/113 (100%)

Query: 1   MGSTSSMLTQYDIEEVQDHCDHAFSQQEIVSLYHRFCQLDRNGGGFVSAEEFMTVPEFAV 60
           MGSTSSMLTQYDIEEVQDHCDHAFSQQEIVSLYHRFCQLDRNGGGFVSAEEFMTVPEFAV
Sbjct: 1   MGSTSSMLTQYDIEEVQDHCDHAFSQQEIVSLYHRFCQLDRNGGGFVSAEEFMTVPEFAV 60

Query: 61  NPLSQRLLRMLDGLNFKEFVAFLSAFSPRASLQQKIEWFMTLTAMGRWHLMIS 113
           NPLSQRLLRMLDGLNFKEFVAFLSAFSPRASLQQKIEWFMTLTAMGRWHLMIS
Sbjct: 61  NPLSQRLLRMLDGLNFKEFVAFLSAFSPRASLQQKIEWFMTLTAMGRWHLMIS 113



>Os02g0802400 EF-Hand type domain containing protein
          Length = 100

 Score =  161 bits (407), Expect = 1e-40,   Method: Compositional matrix adjust.
 Identities = 71/97 (73%), Positives = 89/97 (91%)

Query: 1  MGSTSSMLTQYDIEEVQDHCDHAFSQQEIVSLYHRFCQLDRNGGGFVSAEEFMTVPEFAV 60
          MG+ SSMLTQYDIEEVQ+HC++ FSQQEIVSLY RFCQLDR+  GF+S +EF+++PEF++
Sbjct: 1  MGNASSMLTQYDIEEVQEHCNYLFSQQEIVSLYERFCQLDRSAKGFISEDEFLSIPEFSL 60

Query: 61 NPLSQRLLRMLDGLNFKEFVAFLSAFSPRASLQQKIE 97
          NPLS+RLLRM+DGLNFK+FV+FLS FS +AS+QQKIE
Sbjct: 61 NPLSKRLLRMVDGLNFKDFVSFLSTFSAKASVQQKIE 97



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.135    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,475,301
Number of extensions: 116957
Number of successful extensions: 387
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 387
Number of HSP's successfully gapped: 2
Length of query: 113
Length of database: 17,035,801
Length adjustment: 80
Effective length of query: 33
Effective length of database: 12,858,681
Effective search space: 424336473
Effective search space used: 424336473
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 149 (62.0 bits)