BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0433600 Os08g0433600|AK067255
         (200 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0433600  Protein of unknown function DUF962 family protein   231   3e-61
Os02g0578800  Protein of unknown function DUF962 family protein    89   2e-18

>Os08g0433600 Protein of unknown function DUF962 family protein
          Length = 200

 Score =  231 bits (589), Expect = 3e-61,   Method: Compositional matrix adjust.
 Identities = 125/184 (67%), Positives = 125/184 (67%)

Query: 17  GVLDLEAQFAFFRSQHRHPVNAAAHALLAWPILFTGLLVLHXXXXXXXXXXXXXXXXXXX 76
           GVLDLEAQFAFFRSQHRHPVNAAAHALLAWPILFTGLLVLH                   
Sbjct: 17  GVLDLEAQFAFFRSQHRHPVNAAAHALLAWPILFTGLLVLHFLPSPPALPLDPALALALA 76

Query: 77  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKAALATQLFCWTWQFLGHGL 136
                                                   KAALATQLFCWTWQFLGHGL
Sbjct: 77  YAAAYVAADRRAGALAGLLLAAGWAASRALAARLGFALAWKAALATQLFCWTWQFLGHGL 136

Query: 137 FEKRGPAVGDLPEVFLMEPFLILLQILNKQFGYEPYPGFSKNVDKKMEAILRENREELKQ 196
           FEKRGPAVGDLPEVFLMEPFLILLQILNKQFGYEPYPGFSKNVDKKMEAILRENREELKQ
Sbjct: 137 FEKRGPAVGDLPEVFLMEPFLILLQILNKQFGYEPYPGFSKNVDKKMEAILRENREELKQ 196

Query: 197 RKAT 200
           RKAT
Sbjct: 197 RKAT 200



>Os02g0578800 Protein of unknown function DUF962 family protein
          Length = 187

 Score = 89.0 bits (219), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 45/84 (53%), Positives = 57/84 (67%), Gaps = 5/84 (5%)

Query: 117 KAALATQLFCWTWQFLGHGLFEKRGPAVGD-LPEVFLMEPFLILLQILNKQFGYEPYPGF 175
           K  L  QLFCWT QF+GHG+FEKR PA+ D L + FLM PF +LL+ L+K  GYEPYPGF
Sbjct: 104 KVVLVAQLFCWTMQFIGHGVFEKRAPALLDNLVQAFLMAPFFVLLEALHKFVGYEPYPGF 163

Query: 176 SKNVDKKMEAILRENREELKQRKA 199
              V K    ++ E R+E + +KA
Sbjct: 164 HAKVQK----LIDEARKEWEDKKA 183



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.327    0.141    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,178,044
Number of extensions: 128623
Number of successful extensions: 294
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 293
Number of HSP's successfully gapped: 3
Length of query: 200
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 104
Effective length of database: 12,023,257
Effective search space: 1250418728
Effective search space used: 1250418728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 153 (63.5 bits)