BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0375900 Os08g0375900|AK069663
         (250 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0375900  Similar to Nudix hydrolase 22, chloroplast pre...   421   e-118
Os08g0376200  Similar to Nudix hydrolase 15, mitochondrial p...   246   8e-66
Os05g0209400  Similar to Nudix hydrolase 15, mitochondrial p...   185   3e-47

>Os08g0375900 Similar to Nudix hydrolase 22, chloroplast precursor (EC 3.6.1.-)
           (AtNUDT22)
          Length = 250

 Score =  421 bits (1083), Expect = e-118,   Method: Compositional matrix adjust.
 Identities = 213/250 (85%), Positives = 213/250 (85%)

Query: 1   MSTDLTIPPSMEAPGADIQALIQRLRLHXXXXXXXXXXXXXXXXXXXXXXELLFRPRRAA 60
           MSTDLTIPPSMEAPGADIQALIQRLRLH                      ELLFRPRRAA
Sbjct: 1   MSTDLTIPPSMEAPGADIQALIQRLRLHRPRPSPYAAAAAPSPDAAAPPAELLFRPRRAA 60

Query: 61  VLICLFRGAAGELRVLLTKRSSKLSSHSGEVSLPXXXXXXXXXXXXXXXLREAKEEIGLD 120
           VLICLFRGAAGELRVLLTKRSSKLSSHSGEVSLP               LREAKEEIGLD
Sbjct: 61  VLICLFRGAAGELRVLLTKRSSKLSSHSGEVSLPGGKAEEGDADDAATALREAKEEIGLD 120

Query: 121 PASVTVVASLEHFLSKHLLVVVPVVGILSDIQAFKPVLNVDEVDSIFDVPLEMFLKDEKR 180
           PASVTVVASLEHFLSKHLLVVVPVVGILSDIQAFKPVLNVDEVDSIFDVPLEMFLKDEKR
Sbjct: 121 PASVTVVASLEHFLSKHLLVVVPVVGILSDIQAFKPVLNVDEVDSIFDVPLEMFLKDEKR 180

Query: 181 TSEEREWMGQEFTIHYFNYEKGSEKYVIWGLTAGILIHAASVVFQRPPDFPARTVQFNLP 240
           TSEEREWMGQEFTIHYFNYEKGSEKYVIWGLTAGILIHAASVVFQRPPDFPARTVQFNLP
Sbjct: 181 TSEEREWMGQEFTIHYFNYEKGSEKYVIWGLTAGILIHAASVVFQRPPDFPARTVQFNLP 240

Query: 241 KYSKECSSMP 250
           KYSKECSSMP
Sbjct: 241 KYSKECSSMP 250



>Os08g0376200 Similar to Nudix hydrolase 15, mitochondrial precursor (EC 3.6.1.-)
           (AtNUDT15)
          Length = 163

 Score =  246 bits (629), Expect = 8e-66,   Method: Compositional matrix adjust.
 Identities = 121/155 (78%), Positives = 130/155 (83%)

Query: 88  SGEVSLPXXXXXXXXXXXXXXXLREAKEEIGLDPASVTVVASLEHFLSKHLLVVVPVVGI 147
           SGEV+LP               LREAKEEIGLDP+ VTVVASLEHFLSKHLLVVVP+VGI
Sbjct: 9   SGEVALPGGKAEEGDADDAATALREAKEEIGLDPSLVTVVASLEHFLSKHLLVVVPIVGI 68

Query: 148 LSDIQAFKPVLNVDEVDSIFDVPLEMFLKDEKRTSEEREWMGQEFTIHYFNYEKGSEKYV 207
           LSDI+AFKPVLNVDEVD IFDVPLEMFLKDE RTSEERE MGQ FTIHYFNYEK ++KY+
Sbjct: 69  LSDIEAFKPVLNVDEVDDIFDVPLEMFLKDENRTSEEREKMGQTFTIHYFNYEKENQKYL 128

Query: 208 IWGLTAGILIHAASVVFQRPPDFPARTVQFNLPKY 242
           IWGLTA ILIHAASVV+QRPPDFP R V FNLPK+
Sbjct: 129 IWGLTARILIHAASVVYQRPPDFPERRVHFNLPKF 163



>Os05g0209400 Similar to Nudix hydrolase 15, mitochondrial precursor (EC 3.6.1.-)
           (AtNUDT15)
          Length = 258

 Score =  185 bits (469), Expect = 3e-47,   Method: Compositional matrix adjust.
 Identities = 102/191 (53%), Positives = 121/191 (63%), Gaps = 6/191 (3%)

Query: 59  AAVLICLFRGA-AGELRVLLTKRSSKLSSHSGEVSLPXXXXXXXXXXXXXXXLREAKEEI 117
           AAVL+CLF     G+ RV+LTKR++ LSSHSGEVSLP               LREAKEEI
Sbjct: 66  AAVLVCLFEDPHGGDPRVILTKRAASLSSHSGEVSLPGGKVEEGDADATATALREAKEEI 125

Query: 118 GLDPASVTVVASLEHFLSKHLLVVVPVVGILSDIQAFKPVLNVDEVDSIFDVPLEMFLKD 177
           GLDPA V++V  LE FLSK+ L V PV+GILSD   FKPVLN  EV  IFD PLEMFLKD
Sbjct: 126 GLDPALVSIVTVLEPFLSKNGLHVTPVIGILSDKALFKPVLNESEVADIFDAPLEMFLKD 185

Query: 178 EKRTSEEREWMGQEFTIHYFNYEKGSEKYVIWGLTAGILIHAASVVFQRPPDFPARTVQF 237
           + R ++E  WMG    +  F Y+   + +VIWGLTA IL  AA+VV  R P F    V+F
Sbjct: 186 DNRKTQESNWMGMNIPVQSFEYQSEDKTFVIWGLTAHILTRAAAVVLPREPSF----VEF 241

Query: 238 NLPKYSKECSS 248
             P+Y    S 
Sbjct: 242 R-PRYVNSPSG 251



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,950,997
Number of extensions: 229522
Number of successful extensions: 382
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 381
Number of HSP's successfully gapped: 3
Length of query: 250
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 152
Effective length of database: 11,918,829
Effective search space: 1811662008
Effective search space used: 1811662008
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)