BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0278900 Os08g0278900|AK100085
         (217 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0278900  MIR domain containing protein                       434   e-122
Os08g0440500  MIR domain containing protein                       323   8e-89
AK109994                                                           85   3e-17

>Os08g0278900 MIR domain containing protein
          Length = 217

 Score =  434 bits (1115), Expect = e-122,   Method: Compositional matrix adjust.
 Identities = 208/217 (95%), Positives = 208/217 (95%)

Query: 1   MAAASFAIAXXXXXXXXXPEASPAQSYAADPDNVVEITYGSAIKLMHERTKFRLHSHDVP 60
           MAAASFAIA         PEASPAQSYAADPDNVVEITYGSAIKLMHERTKFRLHSHDVP
Sbjct: 1   MAAASFAIALLLYLGLDLPEASPAQSYAADPDNVVEITYGSAIKLMHERTKFRLHSHDVP 60

Query: 61  YGSGSGQQSVTSFPNVDDSNSYWIVRPQPDTSAKQGDPITHGTVVRLQHMRTRKWLHSHM 120
           YGSGSGQQSVTSFPNVDDSNSYWIVRPQPDTSAKQGDPITHGTVVRLQHMRTRKWLHSHM
Sbjct: 61  YGSGSGQQSVTSFPNVDDSNSYWIVRPQPDTSAKQGDPITHGTVVRLQHMRTRKWLHSHM 120

Query: 121 HASPITGNLEVSCFGGENESDTGDYWRLEIEGSGKSWRQNQKIRLRHVDTGGYLHSHDRK 180
           HASPITGNLEVSCFGGENESDTGDYWRLEIEGSGKSWRQNQKIRLRHVDTGGYLHSHDRK
Sbjct: 121 HASPITGNLEVSCFGGENESDTGDYWRLEIEGSGKSWRQNQKIRLRHVDTGGYLHSHDRK 180

Query: 181 YTRIAGGQQEVCGVGDKRPDNVWLAAEGVYLPVNQQK 217
           YTRIAGGQQEVCGVGDKRPDNVWLAAEGVYLPVNQQK
Sbjct: 181 YTRIAGGQQEVCGVGDKRPDNVWLAAEGVYLPVNQQK 217



>Os08g0440500 MIR domain containing protein
          Length = 217

 Score =  323 bits (827), Expect = 8e-89,   Method: Compositional matrix adjust.
 Identities = 144/183 (78%), Positives = 165/183 (90%)

Query: 35  VEITYGSAIKLMHERTKFRLHSHDVPYGSGSGQQSVTSFPNVDDSNSYWIVRPQPDTSAK 94
           VE+ YGS IKLMHE+TK RLHSHDVPYGSGSGQQSVT FP VDDSNSYWIVRP PD+SAK
Sbjct: 35  VEVAYGSTIKLMHEKTKHRLHSHDVPYGSGSGQQSVTGFPEVDDSNSYWIVRPSPDSSAK 94

Query: 95  QGDPITHGTVVRLQHMRTRKWLHSHMHASPITGNLEVSCFGGENESDTGDYWRLEIEGSG 154
           QGD I  G+++RLQHMRTRKWLHSH+HASP++GNLEVSCFGG+ +SDTGDYWRLEIEG G
Sbjct: 95  QGDAIETGSIIRLQHMRTRKWLHSHLHASPLSGNLEVSCFGGDGQSDTGDYWRLEIEGGG 154

Query: 155 KSWRQNQKIRLRHVDTGGYLHSHDRKYTRIAGGQQEVCGVGDKRPDNVWLAAEGVYLPVN 214
           K W+Q+QK+RLRHVDTGGYLHSH++KY R+ GGQQEVCGV +KR +N+WLA EGVYLPVN
Sbjct: 155 KLWKQDQKVRLRHVDTGGYLHSHNKKYNRLGGGQQEVCGVREKRAENIWLATEGVYLPVN 214

Query: 215 QQK 217
           + K
Sbjct: 215 KSK 217



>AK109994 
          Length = 649

 Score = 85.1 bits (209), Expect = 3e-17,   Method: Compositional matrix adjust.
 Identities = 68/204 (33%), Positives = 100/204 (49%), Gaps = 32/204 (15%)

Query: 32  DNVVEITYGSAIKLMHERTKF-RLHSHDVPYGSGSGQQSVTSFPNVDDSNSY-------- 82
           D   ++ +G+ I L H  T+   LHSH   Y +GS QQ +T +P+ D++N +        
Sbjct: 347 DVPADVAFGARISLRHHNTQGGYLHSHQHMYPTGSKQQQITLYPHKDENNVFLVENQTQP 406

Query: 83  --WIVRPQPDTSAK------QGDP--ITHGTVVRLQHMRTRKWLHSHMHASPITG---NL 129
             W   P  +TS        + DP  +  G+V+RL H+ T + +HSH H  P+T      
Sbjct: 407 IDWAADPTGNTSVAGPMAWDKFDPEWVVDGSVIRLYHVITDRRVHSHDHRPPVTEADWQN 466

Query: 130 EVSCFGGEN-ESDTGDYWRLEIEGS---GKSWRQ-----NQKIRLRHVDTGGYLHSHDRK 180
           EVS +G +N E D  D +R+EI  S   GK  ++       K +L H+ TG  L SH  K
Sbjct: 467 EVSAYGYQNFEGDANDLFRVEIVKSMSDGKEAKERLRTIQTKFKLVHLMTGCVLFSHKVK 526

Query: 181 YTRIAGGQQEV-CGVGDKRPDNVW 203
                  QQEV C  G   P+++W
Sbjct: 527 LPDWGFEQQEVTCAKGGTLPNSIW 550



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.131    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,779,969
Number of extensions: 423603
Number of successful extensions: 715
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 699
Number of HSP's successfully gapped: 3
Length of query: 217
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 120
Effective length of database: 11,971,043
Effective search space: 1436525160
Effective search space used: 1436525160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)