BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0254500 Os08g0254500|AK121121
         (556 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0254500  Similar to Preprotein translocase secY subunit...   868   0.0  
Os05g0397700  SecY protein family protein                         117   2e-26

>Os08g0254500 Similar to Preprotein translocase secY subunit, chloroplast
           precursor (CpSecY)
          Length = 556

 Score =  868 bits (2243), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 455/556 (81%), Positives = 455/556 (81%)

Query: 1   MAAMATPQQCWLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
           MAAMATPQQCWL                                                
Sbjct: 1   MAAMATPQQCWLPTRARAPPPPPPRAPSSASPVSGAPASLRFRGRRAASASASAARRRRG 60

Query: 61  XXXPCSPRCALETAGPGFDPLGLYNDGPSRNDTQSPLSNFFGVLSPVFGSSSGGRKEKSY 120
              PCSPRCALETAGPGFDPLGLYNDGPSRNDTQSPLSNFFGVLSPVFGSSSGGRKEKSY
Sbjct: 61  ASLPCSPRCALETAGPGFDPLGLYNDGPSRNDTQSPLSNFFGVLSPVFGSSSGGRKEKSY 120

Query: 121 GRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYLALSRLGIYIPLGGVNRDAFAGNLDQN 180
           GR                              YLALSRLGIYIPLGGVNRDAFAGNLDQN
Sbjct: 121 GRGAAAAIEDSSIDIGDFFKGPLPGKFLKLLGYLALSRLGIYIPLGGVNRDAFAGNLDQN 180

Query: 181 SLLGTLDSFSGGGIGRLGICSLGIVPFINAQIVFQLLAQLYPKLQDLQKKEGEAGRKKVL 240
           SLLGTLDSFSGGGIGRLGICSLGIVPFINAQIVFQLLAQLYPKLQDLQKKEGEAGRKKVL
Sbjct: 181 SLLGTLDSFSGGGIGRLGICSLGIVPFINAQIVFQLLAQLYPKLQDLQKKEGEAGRKKVL 240

Query: 241 QYTRYASVGFAIVQAIGQVLFLRPYVNDFXXXXXXXXXXXXXXXXXXXXFIGERISDLKL 300
           QYTRYASVGFAIVQAIGQVLFLRPYVNDF                    FIGERISDLKL
Sbjct: 241 QYTRYASVGFAIVQAIGQVLFLRPYVNDFSTEWVLTSVTLLTLGSVFTTFIGERISDLKL 300

Query: 301 GNGTSLLIFTSIISYLPASFGRTVAQAFQDGNYVGLLTIILSFLFLVLGIVYVQEAERKI 360
           GNGTSLLIFTSIISYLPASFGRTVAQAFQDGNYVGLLTIILSFLFLVLGIVYVQEAERKI
Sbjct: 301 GNGTSLLIFTSIISYLPASFGRTVAQAFQDGNYVGLLTIILSFLFLVLGIVYVQEAERKI 360

Query: 361 PLNYASRYSSRSGGLQRSAYLPFKVNSSGVMPIIFSTSSLALPGTLARFTGLEFLKKAAI 420
           PLNYASRYSSRSGGLQRSAYLPFKVNSSGVMPIIFSTSSLALPGTLARFTGLEFLKKAAI
Sbjct: 361 PLNYASRYSSRSGGLQRSAYLPFKVNSSGVMPIIFSTSSLALPGTLARFTGLEFLKKAAI 420

Query: 421 SLNPGGALYIPTNVLLIAFFNYYYTFLQLDPDDLSEQLKRQGASIPLVRPGKSTAAFIKT 480
           SLNPGGALYIPTNVLLIAFFNYYYTFLQLDPDDLSEQLKRQGASIPLVRPGKSTAAFIKT
Sbjct: 421 SLNPGGALYIPTNVLLIAFFNYYYTFLQLDPDDLSEQLKRQGASIPLVRPGKSTAAFIKT 480

Query: 481 VLSNISVLGSAFLAVLAAGPSVVEQITHLTAFRGFAGTSVLILVGCATDTARKVQAEIIS 540
           VLSNISVLGSAFLAVLAAGPSVVEQITHLTAFRGFAGTSVLILVGCATDTARKVQAEIIS
Sbjct: 481 VLSNISVLGSAFLAVLAAGPSVVEQITHLTAFRGFAGTSVLILVGCATDTARKVQAEIIS 540

Query: 541 QKYKNIEFYDVNRFDQ 556
           QKYKNIEFYDVNRFDQ
Sbjct: 541 QKYKNIEFYDVNRFDQ 556



>Os05g0397700 SecY protein family protein
          Length = 542

 Score =  117 bits (294), Expect = 2e-26,   Method: Compositional matrix adjust.
 Identities = 107/401 (26%), Positives = 184/401 (45%), Gaps = 25/401 (6%)

Query: 156 LSRLGIYIPLGGVNRDAFAGNLDQNSLLGTLDSFSGGGIGRLGICSLGIVPFINAQIVFQ 215
           LSR+G ++PL G +R     +    + L   D        +L    LGI   I+A IV Q
Sbjct: 131 LSRVGYFVPLPGFDRRLIPDSYLSFAPLPADDLGDFSSELKLSFFQLGISHQISASIVMQ 190

Query: 216 LLAQLYPKLQDLQKKEGEAGRKKVLQYTRYASVGFAIVQAIGQVLF---LRPYVNDFXXX 272
           +L  + P L+ L +KEG  G +K+  Y  + S+GFA+V A     +      Y   +   
Sbjct: 191 VLCHVLPSLEKL-RKEGLDGHEKIKGYIWWLSLGFALVAAFTVSCYSLQYSIYAASYRVK 249

Query: 273 XXXXXXXXXXXXXXXXXFIGERISDLKLGNGTSLLIFTSIISYLPASFGRTVAQAFQDGN 332
                            +I + IS+   G+G+SL+I   I++    +  + + Q    GN
Sbjct: 250 HVMITSLFLVLGAMTMTWICDTISESGFGHGSSLIICVGILTGYTDTLHKMLTQF--SGN 307

Query: 333 YVGLLTIIL----SFLFLVLGIVYVQEAERKIPLNY-----ASRYSSRSGGL-QRSAYLP 382
           +      IL    +F+ + +G V V E  RKI L Y     AS   S S  + +   Y+P
Sbjct: 308 WYSCWPYILGIAGTFILVTMGAVLVTEGCRKIKLQYYGFKLASGARSESSPVTEVEPYIP 367

Query: 383 FKVNSSGVMPIIFSTSSLALPGTLARFTGLEFLKKAAISLNP------GGALYIPTNVLL 436
           F +N +G+ P++ ++  LA P  +A   G +F +    +LNP      G  +Y  T   L
Sbjct: 368 FNINPTGMQPLLTTSYLLAFPSIMASIFGTQFWESLKETLNPKTSVGGGPWVYYLTYAFL 427

Query: 437 IAFFNYYYTFLQLDPDDLSEQLKRQGASIPLVRPGKSTAAFIKTVLSNISVLGSAFLAVL 496
           +  FN +   +   P ++S+ L +  A +P ++PG++T  ++  + ++    G   L++L
Sbjct: 428 VFVFNIFD--IANLPKEISDYLNKMSARVPKIKPGRATVEYLTKIQTSTRFWGGILLSLL 485

Query: 497 AAGPSVVEQ-ITHLTAFRGFAGTSVLILVGCATDTARKVQA 536
           A    ++++ +  +        TSVLI+VG   +  R  QA
Sbjct: 486 ATSSLLLDRYLRQINEGFSIGFTSVLIIVGSIIELRRSYQA 526



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.140    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,599,465
Number of extensions: 541567
Number of successful extensions: 1029
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1022
Number of HSP's successfully gapped: 2
Length of query: 556
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 450
Effective length of database: 11,501,117
Effective search space: 5175502650
Effective search space used: 5175502650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 159 (65.9 bits)