BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0230600 Os08g0230600|AK111371
(81 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0230600 LIM, zinc-binding domain containing protein 148 9e-37
Os04g0606200 Conserved hypothetical protein 96 6e-21
Os02g0463300 69 7e-13
Os06g0660400 Conserved hypothetical protein 68 2e-12
>Os08g0230600 LIM, zinc-binding domain containing protein
Length = 81
Score = 148 bits (373), Expect = 9e-37, Method: Compositional matrix adjust.
Identities = 75/75 (100%), Positives = 75/75 (100%)
Query: 1 MAMVEAARRAMGWCGAEEERETASRKAPGACPRCGGAVVATDVESSRRILCLPLCLRNKK 60
MAMVEAARRAMGWCGAEEERETASRKAPGACPRCGGAVVATDVESSRRILCLPLCLRNKK
Sbjct: 1 MAMVEAARRAMGWCGAEEERETASRKAPGACPRCGGAVVATDVESSRRILCLPLCLRNKK 60
Query: 61 KFSCSRCRRALVAIV 75
KFSCSRCRRALVAIV
Sbjct: 61 KFSCSRCRRALVAIV 75
>Os04g0606200 Conserved hypothetical protein
Length = 71
Score = 95.9 bits (237), Expect = 6e-21, Method: Compositional matrix adjust.
Identities = 45/62 (72%), Positives = 52/62 (83%)
Query: 13 WCGAEEERETASRKAPGACPRCGGAVVATDVESSRRILCLPLCLRNKKKFSCSRCRRALV 72
CGA EER + KAPGACPRCGGAVVATDVES RRILCLPLC+++K+K+SCSRC R LV
Sbjct: 8 MCGAAEERVVGTHKAPGACPRCGGAVVATDVESERRILCLPLCVKSKRKYSCSRCLRRLV 67
Query: 73 AI 74
+
Sbjct: 68 TL 69
>Os02g0463300
Length = 67
Score = 68.9 bits (167), Expect = 7e-13, Method: Compositional matrix adjust.
Identities = 33/58 (56%), Positives = 42/58 (72%)
Query: 17 EEERETASRKAPGACPRCGGAVVATDVESSRRILCLPLCLRNKKKFSCSRCRRALVAI 74
E ER A+ +APG CP CGG VVATDVES+ R+ LPLC R +++F CS C R LV++
Sbjct: 9 EPERVVATYQAPGRCPYCGGGVVATDVESAPRLCFLPLCFRLRRRFFCSLCSRRLVSV 66
>Os06g0660400 Conserved hypothetical protein
Length = 68
Score = 67.8 bits (164), Expect = 2e-12, Method: Compositional matrix adjust.
Identities = 31/59 (52%), Positives = 41/59 (69%)
Query: 16 AEEERETASRKAPGACPRCGGAVVATDVESSRRILCLPLCLRNKKKFSCSRCRRALVAI 74
+EE+ +KAPG+CP CGG V ATDVE+ + LPLCL NK++FSC+ C R LV+
Sbjct: 4 GKEEKLLGVQKAPGSCPYCGGGVAATDVEAKWVLCFLPLCLNNKRRFSCTACNRRLVSY 62
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.326 0.133 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,557,035
Number of extensions: 83221
Number of successful extensions: 490
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 490
Number of HSP's successfully gapped: 4
Length of query: 81
Length of database: 17,035,801
Length adjustment: 51
Effective length of query: 30
Effective length of database: 14,372,887
Effective search space: 431186610
Effective search space used: 431186610
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 149 (62.0 bits)