BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0224700 Os08g0224700|AK121754
         (987 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0224700  Similar to 26S proteasome subunit RPN2a            1815   0.0  
Os04g0608500  Similar to 26S proteasome subunit RPN2a             726   0.0  

>Os08g0224700 Similar to 26S proteasome subunit RPN2a
          Length = 987

 Score = 1815 bits (4701), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 898/975 (92%), Positives = 898/975 (92%)

Query: 13  GLLAMLQEPAAELKLHALANLNSLVHVFWPEISTSVPAIESLYEDEEFDQRQLAALVVSK 72
           GLLAMLQEPAAELKLHALANLNSLVHVFWPEISTSVPAIESLYEDEEFDQRQLAALVVSK
Sbjct: 13  GLLAMLQEPAAELKLHALANLNSLVHVFWPEISTSVPAIESLYEDEEFDQRQLAALVVSK 72

Query: 73  VFYYLGELDDSLSYALGAGPLFDPSEGSDYAQTLLAKALDEYASIRSKSLKAAGEGDEKM 132
           VFYYLGELDDSLSYALGAGPLFDPSEGSDYAQTLLAKALDEYASIRSKSLKAAGEGDEKM
Sbjct: 73  VFYYLGELDDSLSYALGAGPLFDPSEGSDYAQTLLAKALDEYASIRSKSLKAAGEGDEKM 132

Query: 133 DPRLEAIVERMLDKCILDGKYQQAMGMSLECKRLDKLEEAISRSDNLNGSLSYSIILSHQ 192
           DPRLEAIVERMLDKCILDGKYQQAMGMSLECKRLDKLEEAISRSDNLNGSLSYSIILSHQ
Sbjct: 133 DPRLEAIVERMLDKCILDGKYQQAMGMSLECKRLDKLEEAISRSDNLNGSLSYSIILSHQ 192

Query: 193 YVSHREYRCEILRCLVKIYQTLPNPDYLSICQCLMFLGEPETVGNILGKLLSGSKDDALL 252
           YVSHREYRCEILRCLVKIYQTLPNPDYLSICQCLMFLGEPETVGNILGKLLSGSKDDALL
Sbjct: 193 YVSHREYRCEILRCLVKIYQTLPNPDYLSICQCLMFLGEPETVGNILGKLLSGSKDDALL 252

Query: 253 AFQIAFDLVENENQAFLLNVKNHLDTLSLQTSAQTLPSDQTAATEPSGDAQMTDDAITPN 312
           AFQIAFDLVENENQAFLLNVKNHLDTLSLQTSAQTLPSDQTAATEPSGDAQMTDDAITPN
Sbjct: 253 AFQIAFDLVENENQAFLLNVKNHLDTLSLQTSAQTLPSDQTAATEPSGDAQMTDDAITPN 312

Query: 313 GNAHTVDPNEVAHADRLTKIKGILSGETSIQLTLQFLYSHNRSDLLILKTIKQALEMRNS 372
           GNAHTVDPNEVAHADRLTKIKGILSGETSIQLTLQFLYSHNRSDLLILKTIKQALEMRNS
Sbjct: 313 GNAHTVDPNEVAHADRLTKIKGILSGETSIQLTLQFLYSHNRSDLLILKTIKQALEMRNS 372

Query: 373 VCHSATICSNAIMHAGTTVDTFLRENLEWLSRATNWAKFSATAGLGVIHRGHLTQGRALM 432
           VCHSATICSNAIMHAGTTVDTFLRENLEWLSRATNWAKFSATAGLGVIHRGHLTQGRALM
Sbjct: 373 VCHSATICSNAIMHAGTTVDTFLRENLEWLSRATNWAKFSATAGLGVIHRGHLTQGRALM 432

Query: 433 APYLPQSGAASGGSPYSEGGALYALGLIHANHGEGIKQFLRESLRNTSSEVVQHXXXXXX 492
           APYLPQSGAASGGSPYSEGGALYALGLIHANHGEGIKQFLRESLRNTSSEVVQH      
Sbjct: 433 APYLPQSGAASGGSPYSEGGALYALGLIHANHGEGIKQFLRESLRNTSSEVVQHGACLGL 492

Query: 493 XXXXXXXXDEEIFEDVKNILYTDSAVXXXXXXXXXXXXXXXXXSEKAGEMLAYAHDTQHE 552
                   DEEIFEDVKNILYTDSAV                 SEKAGEMLAYAHDTQHE
Sbjct: 493 GLAALGTADEEIFEDVKNILYTDSAVAGEAAGIGMGLLMAGTASEKAGEMLAYAHDTQHE 552

Query: 553 KIIRGLSLGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAYRGTANNKAIHQL 612
           KIIRGLSLGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAYRGTANNKAIHQL
Sbjct: 553 KIIRGLSLGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAYRGTANNKAIHQL 612

Query: 613 LHFAVSDVSDDVRRTAVLALGFVLYNEPEQTPRIVSLLSESYNPHVRYGAALAVGISCAG 672
           LHFAVSDVSDDVRRTAVLALGFVLYNEPEQTPRIVSLLSESYNPHVRYGAALAVGISCAG
Sbjct: 613 LHFAVSDVSDDVRRTAVLALGFVLYNEPEQTPRIVSLLSESYNPHVRYGAALAVGISCAG 672

Query: 673 TGLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNESYDSRVGTFRRQLEKIILDKHE 732
           TGLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNESYDSRVGTFRRQLEKIILDKHE
Sbjct: 673 TGLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNESYDSRVGTFRRQLEKIILDKHE 732

Query: 733 DTMSKMGAIMATGILDAGGRNVTIRLQSRSKHDKLTAVVGLAVFTQFWYWYPLTYFISLA 792
           DTMSKMGAIMATGILDAGGRNVTIRLQSRSKHDKLTAVVGLAVFTQFWYWYPLTYFISLA
Sbjct: 733 DTMSKMGAIMATGILDAGGRNVTIRLQSRSKHDKLTAVVGLAVFTQFWYWYPLTYFISLA 792

Query: 793 FSPTAFIGLNSDLKVPKFEFLSNTKPSLFDYPKPTTQQTTTASVKLPTAILSTYXXXXXX 852
           FSPTAFIGLNSDLKVPKFEFLSNTKPSLFDYPKPTTQQTTTASVKLPTAILSTY      
Sbjct: 793 FSPTAFIGLNSDLKVPKFEFLSNTKPSLFDYPKPTTQQTTTASVKLPTAILSTYAKAKSR 852

Query: 853 XXXXXXXXXXXXXXXPPSEDASTSMQVDGVAEKKAPEPEPTFQILTNPARVVPAQEKFIK 912
                          PPSEDASTSMQVDGVAEKKAPEPEPTFQILTNPARVVPAQEKFIK
Sbjct: 853 AKKEAESKAQEKAEAPPSEDASTSMQVDGVAEKKAPEPEPTFQILTNPARVVPAQEKFIK 912

Query: 913 FLEGGRYEPVKLAPSGFVLLRDLKPTETEELVLTDXXXXXXXXXXXXXXXXXXXXXXXXX 972
           FLEGGRYEPVKLAPSGFVLLRDLKPTETEELVLTD                         
Sbjct: 913 FLEGGRYEPVKLAPSGFVLLRDLKPTETEELVLTDAPATQATNAAAATAGQQGSGAAAMA 972

Query: 973 VDEEPLPPQPFEYTS 987
           VDEEPLPPQPFEYTS
Sbjct: 973 VDEEPLPPQPFEYTS 987



>Os04g0608500 Similar to 26S proteasome subunit RPN2a
          Length = 461

 Score =  726 bits (1873), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 365/462 (79%), Positives = 385/462 (83%), Gaps = 18/462 (3%)

Query: 541 EMLAYAHDTQHEKIIRGLSLGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAY 600
           EMLAYAHDTQHEKIIRGLSLGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAY
Sbjct: 3   EMLAYAHDTQHEKIIRGLSLGIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAY 62

Query: 601 RGTANNKAIHQLLHFAVSDVSDDVRRTAVLALGFVLYNEPEQTPRIVSLLSESYNPHVRY 660
           RGTANNKAIHQLLHFAVSDVSDDVRRTAV+ALGFVLYNEPEQTPRIVSLLSESYNPHVRY
Sbjct: 63  RGTANNKAIHQLLHFAVSDVSDDVRRTAVMALGFVLYNEPEQTPRIVSLLSESYNPHVRY 122

Query: 661 GAALAVGISCAGTGLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNESYDSRVGTFR 720
           GAALAVGISCAG+GLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNES+DSRVGTFR
Sbjct: 123 GAALAVGISCAGSGLSDAISLLEPLTSDVVDFVRQGALIAMAMVMIQTNESFDSRVGTFR 182

Query: 721 RQLEKIILDKHEDTMSKMGAIMATGILDAGGRNVTIRLQSRSKHDKLTAVVGLAVFTQFW 780
           RQLEKIILDKHEDTMSKMGAI+A+GILDAGGRNVTI+L SR+KHDKLTAVVGLAVF+QFW
Sbjct: 183 RQLEKIILDKHEDTMSKMGAILASGILDAGGRNVTIKLLSRNKHDKLTAVVGLAVFSQFW 242

Query: 781 YWYPLTYFISLAFSPTAFIGLNSDLKVPKFEFLSNTKPSLFDYPKPTTQQTTTASVKLPT 840
           YWYPL YFISLAFSPTAFIGLNSDLKVPKFEFLS+ KPSLF+YPKPTTQQTTT++VKLPT
Sbjct: 243 YWYPLLYFISLAFSPTAFIGLNSDLKVPKFEFLSHAKPSLFEYPKPTTQQTTTSAVKLPT 302

Query: 841 AILSTYXXXXXXXXXXXXXXXXXXXXXPPSEDASTS---------------MQVDGVAEK 885
           AILSTY                       +EDAS S               MQVD  AEK
Sbjct: 303 AILSTYAKAKSRAKKDAESKANQEKA---TEDASGSSSSKATKTQEKDADAMQVDNAAEK 359

Query: 886 KAPEPEPTFQILTNPARVVPAQEKFIKFLEGGRYEPVKLAPSGFVLLRDLKPTETEELVL 945
           KAPEPEPTFQILTNPARV+P QEKFIKF+EG RY PVK APSGF+LL+D++PTE E L L
Sbjct: 360 KAPEPEPTFQILTNPARVIPTQEKFIKFIEGSRYVPVKPAPSGFILLQDMQPTEAEVLAL 419

Query: 946 TDXXXXXXXXXXXXXXXXXXXXXXXXXVDEEPLPPQPFEYTS 987
           TD                         VD+EP PPQPFEYTS
Sbjct: 420 TDAPSTVAATTGSAAAATGQQASSAMAVDDEPQPPQPFEYTS 461



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.133    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 29,420,801
Number of extensions: 1190005
Number of successful extensions: 3053
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 3044
Number of HSP's successfully gapped: 2
Length of query: 987
Length of database: 17,035,801
Length adjustment: 111
Effective length of query: 876
Effective length of database: 11,240,047
Effective search space: 9846281172
Effective search space used: 9846281172
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 161 (66.6 bits)