BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0180000 Os08g0180000|AK103587
         (369 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0180000  mRNA capping enzyme, large subunit family protein   739   0.0  
Os02g0780600  Methyltransferase type 12 domain containing pr...   106   3e-23

>Os08g0180000 mRNA capping enzyme, large subunit family protein
          Length = 369

 Score =  739 bits (1908), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 356/369 (96%), Positives = 356/369 (96%)

Query: 1   MNKRPRDEPSSSFASAPKRQXXXXXXXXXXXXXSEERSSARRVADHYSARSNQTLEEREN 60
           MNKRPRDEPSSSFASAPKRQ             SEERSSARRVADHYSARSNQTLEEREN
Sbjct: 1   MNKRPRDEPSSSFASAPKRQYGAGGGGYGGHGYSEERSSARRVADHYSARSNQTLEEREN 60

Query: 61  SPIIHLKKLNNWIKSVLIQLYAHPGDCVLDLACGKGGDLIKWDKAKVGYYVGVDIAEGSI 120
           SPIIHLKKLNNWIKSVLIQLYAHPGDCVLDLACGKGGDLIKWDKAKVGYYVGVDIAEGSI
Sbjct: 61  SPIIHLKKLNNWIKSVLIQLYAHPGDCVLDLACGKGGDLIKWDKAKVGYYVGVDIAEGSI 120

Query: 121 KDCMTRYNGDTDQQRRKKFSFPARLICADCYEARLDEHLYEDAPFDICSCQFALHYSWST 180
           KDCMTRYNGDTDQQRRKKFSFPARLICADCYEARLDEHLYEDAPFDICSCQFALHYSWST
Sbjct: 121 KDCMTRYNGDTDQQRRKKFSFPARLICADCYEARLDEHLYEDAPFDICSCQFALHYSWST 180

Query: 181 EARARQALANVSALLRPGGVFIGTMPDANVIIKRLRETDGMEFGNGVYWISFGEEYAEKK 240
           EARARQALANVSALLRPGGVFIGTMPDANVIIKRLRETDGMEFGNGVYWISFGEEYAEKK
Sbjct: 181 EARARQALANVSALLRPGGVFIGTMPDANVIIKRLRETDGMEFGNGVYWISFGEEYAEKK 240

Query: 241 FPASRPFGIKYKFHLEDAVDCPEWVVPFHLFKLLAEEYDLELVLTKNFHEFVHEYLQKPE 300
           FPASRPFGIKYKFHLEDAVDCPEWVVPFHLFKLLAEEYDLELVLTKNFHEFVHEYLQKPE
Sbjct: 241 FPASRPFGIKYKFHLEDAVDCPEWVVPFHLFKLLAEEYDLELVLTKNFHEFVHEYLQKPE 300

Query: 301 FAELMRRLGALGDGRQDQSTLSQDEWEVAYLYLAFVLRKRGQPPSQRRANNANRGKMFLT 360
           FAELMRRLGALGDGRQDQSTLSQDEWEVAYLYLAFVLRKRGQPPSQRRANNANRGKMFLT
Sbjct: 301 FAELMRRLGALGDGRQDQSTLSQDEWEVAYLYLAFVLRKRGQPPSQRRANNANRGKMFLT 360

Query: 361 ENDIDFLGV 369
           ENDIDFLGV
Sbjct: 361 ENDIDFLGV 369



>Os02g0780600 Methyltransferase type 12 domain containing protein
          Length = 339

 Score =  106 bits (264), Expect = 3e-23,   Method: Compositional matrix adjust.
 Identities = 87/287 (30%), Positives = 138/287 (48%), Gaps = 33/287 (11%)

Query: 68  KLNNWIKSVLIQLYAHPGDCVLDLACGKGGDLIKWDKAKVGYYVGVDIAEGSIKDCMTRY 127
           +L  + K+ LI+++A P   V DL C  G D  KW  A++G+Y+G+D +   + D    +
Sbjct: 8   RLYEFAKTALIKIFAFPYATVCDLYCDGGVDTDKWGDAQIGHYIGIDASASGVNDARELW 67

Query: 128 NGDTDQQRRKKFS--FPARLICADCYEARLDEHLYEDAPFDICSCQFALHYSWSTEARAR 185
                + R+K F+  F      AD +EA++ E   +    DI  C   L   + +E  A+
Sbjct: 68  -----ESRKKLFTSEFIELDPSADDFEAQMQEKGIQA---DIVCCMQHLQLCFESEEHAQ 119

Query: 186 QALANVSALLRPGGVFIGTMPDANVIIKRLRETDGMEFGNGV-----------YWISFGE 234
           + L NVS+LL+PGG F+G +PD++ I  + ++        G+           Y I+F  
Sbjct: 120 KLLNNVSSLLKPGGYFVGIIPDSSTIWTKYQKNVEASHNKGLKTVPNSIRSENYVITF-- 177

Query: 235 EYAEKKFPASRPFGIKYKFHL-EDAVDCPEWVVPFHLFKLLAEEYDLELVLTKNFHEFVH 293
           E  E+KFP    FG KY+     +++     +V F  F  LA E  LE V  +N  EF  
Sbjct: 178 EVEEEKFPF---FGKKYQLKFANESMFENHCLVHFPSFMRLAREAGLEYVEIQNLTEFYD 234

Query: 294 EYLQKPEFAELMRRLGALGDGRQD-QSTLSQDEWEVAYLYLAFVLRK 339
           +   + +FA L   LG  G    D +  L    +++  LY  FV +K
Sbjct: 235 D--NRTQFAPL---LGGYGSSLVDPRGKLVARSFDILGLYSTFVFQK 276



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 13,279,426
Number of extensions: 574268
Number of successful extensions: 1123
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1122
Number of HSP's successfully gapped: 2
Length of query: 369
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 267
Effective length of database: 11,709,973
Effective search space: 3126562791
Effective search space used: 3126562791
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)