BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0169800 Os08g0169800|AK100074
         (271 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0169800  Haloacid dehalogenase/epoxide hydrolase family...   444   e-125
Os07g0659400  Haloacid dehalogenase/epoxide hydrolase family...   172   2e-43
Os03g0356498  Haloacid dehalogenase/epoxide hydrolase family...    75   4e-14

>Os08g0169800 Haloacid dehalogenase/epoxide hydrolase family protein
          Length = 271

 Score =  444 bits (1143), Expect = e-125,   Method: Compositional matrix adjust.
 Identities = 219/242 (90%), Positives = 219/242 (90%)

Query: 30  SFSSSGMRCVSXXXXXXXXXRERRSSPAFXXXXXXXXXXXXXXARPVAQTYASLGRRYGM 89
           SFSSSGMRCVS         RERRSSPAF              ARPVAQTYASLGRRYGM
Sbjct: 30  SFSSSGMRCVSAAAGAGGGGRERRSSPAFGGLLLDAGGTLLQLARPVAQTYASLGRRYGM 89

Query: 90  SKSEESIKEGFKRAFSAPWPKTLRYQGDGRPFWRIVVAEATECTNNDYFEEVYEYYAHGD 149
           SKSEESIKEGFKRAFSAPWPKTLRYQGDGRPFWRIVVAEATECTNNDYFEEVYEYYAHGD
Sbjct: 90  SKSEESIKEGFKRAFSAPWPKTLRYQGDGRPFWRIVVAEATECTNNDYFEEVYEYYAHGD 149

Query: 150 AWRLPAGAYETLRDLKDAGVKLAVVSNFDTRLRKLLKDLHVSDMFDAIVVSSEVGHEKPA 209
           AWRLPAGAYETLRDLKDAGVKLAVVSNFDTRLRKLLKDLHVSDMFDAIVVSSEVGHEKPA
Sbjct: 150 AWRLPAGAYETLRDLKDAGVKLAVVSNFDTRLRKLLKDLHVSDMFDAIVVSSEVGHEKPA 209

Query: 210 PEIFKRALDQIGVEASKAVHVGDDETADKAGANAIGLECWLWGQDVRTFSEIQDRILTRF 269
           PEIFKRALDQIGVEASKAVHVGDDETADKAGANAIGLECWLWGQDVRTFSEIQDRILTRF
Sbjct: 210 PEIFKRALDQIGVEASKAVHVGDDETADKAGANAIGLECWLWGQDVRTFSEIQDRILTRF 269

Query: 270 PQ 271
           PQ
Sbjct: 270 PQ 271



>Os07g0659400 Haloacid dehalogenase/epoxide hydrolase family protein
          Length = 304

 Score =  172 bits (436), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 93/195 (47%), Positives = 129/195 (66%), Gaps = 4/195 (2%)

Query: 75  PVAQTYASLGRRYGMSKSEESIKEGFKRAFSAPWPKT-LRYQGDGRPFWRIVVAEATECT 133
           P+AQ Y ++G +YG++ SE+ I   ++RA++ PW ++ LRY  DGRPFW+ +V+ +T C+
Sbjct: 86  PMAQVYRTIGEKYGVNYSEDEILMRYRRAYAQPWGRSRLRYVDDGRPFWQHIVSSSTGCS 145

Query: 134 NNDYFEEVYEYYAHGDAWRL--PAGAYETLRDLKDAGVKLAVVSNFDTRLRKLLKDLHVS 191
           +  YFEE+Y+YY    AW+L  P   Y     L+ AGVK AVVSNFDTRLR LL+ L+  
Sbjct: 146 DLQYFEELYQYYTTAKAWQLCDPDAKY-VFEALRKAGVKTAVVSNFDTRLRPLLQALNCD 204

Query: 192 DMFDAIVVSSEVGHEKPAPEIFKRALDQIGVEASKAVHVGDDETADKAGANAIGLECWLW 251
             FDA+ VS+EV  EKP P IF +A + +GV+  +AVH+GDD   D  GA   G + WLW
Sbjct: 205 HWFDAVAVSAEVAAEKPNPTIFLKACEFLGVKPEEAVHIGDDRRNDLWGARDAGCDAWLW 264

Query: 252 GQDVRTFSEIQDRIL 266
           G DV +F E Q  I+
Sbjct: 265 GSDVYSFKENQAHII 279



>Os03g0356498 Haloacid dehalogenase/epoxide hydrolase family protein
          Length = 157

 Score = 75.5 bits (184), Expect = 4e-14,   Method: Compositional matrix adjust.
 Identities = 48/92 (52%), Positives = 59/92 (64%), Gaps = 1/92 (1%)

Query: 143 EYYAHGDAWRL-PAGAYETLRDLKDAGVKLAVVSNFDTRLRKLLKDLHVSDMFDAIVVSS 201
           +YY    AW+L    A    + L+ AGVK AVVSNFDTRLR LL  L     FDA+ VS+
Sbjct: 9   QYYMTEKAWKLCDPDAENVFKALRKAGVKTAVVSNFDTRLRPLLHVLKCDHWFDAVAVSA 68

Query: 202 EVGHEKPAPEIFKRALDQIGVEASKAVHVGDD 233
           EV  EKP P IF +A + +GV+  +AVHVGDD
Sbjct: 69  EVAAEKPNPTIFLKACESLGVKPEEAVHVGDD 100



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.134    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,453,492
Number of extensions: 295512
Number of successful extensions: 663
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 662
Number of HSP's successfully gapped: 3
Length of query: 271
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 172
Effective length of database: 11,866,615
Effective search space: 2041057780
Effective search space used: 2041057780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 155 (64.3 bits)