BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0138700 Os08g0138700|J065045C12
         (72 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0138700  Similar to Serine/threonine-specific protein k...   147   2e-36
Os06g0283300  Similar to Protein-serine/threonine kinase           92   1e-19

>Os08g0138700 Similar to Serine/threonine-specific protein kinase-like protein
          Length = 72

 Score =  147 bits (370), Expect = 2e-36,   Method: Compositional matrix adjust.
 Identities = 72/72 (100%), Positives = 72/72 (100%)

Query: 1  FSLYRELQKATSNFTTLLGQGAFGPVYKADLSSGETLAVKVLANNSKQGEKEFQTEVSQS 60
          FSLYRELQKATSNFTTLLGQGAFGPVYKADLSSGETLAVKVLANNSKQGEKEFQTEVSQS
Sbjct: 1  FSLYRELQKATSNFTTLLGQGAFGPVYKADLSSGETLAVKVLANNSKQGEKEFQTEVSQS 60

Query: 61 LCARNSDCLVFI 72
          LCARNSDCLVFI
Sbjct: 61 LCARNSDCLVFI 72



>Os06g0283300 Similar to Protein-serine/threonine kinase
          Length = 434

 Score = 91.7 bits (226), Expect = 1e-19,   Method: Composition-based stats.
 Identities = 39/55 (70%), Positives = 53/55 (96%)

Query: 4   YRELQKATSNFTTLLGQGAFGPVYKADLSSGETLAVKVLANNSKQGEKEFQTEVS 58
           Y++LQKAT+NFTT+LGQG+FGPVYKA +++GE +AVKVLA++S+QGE+EFQTEV+
Sbjct: 114 YKDLQKATNNFTTILGQGSFGPVYKAVMATGEVVAVKVLASDSRQGEREFQTEVA 168



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.129    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,974,459
Number of extensions: 54717
Number of successful extensions: 1295
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1400
Number of HSP's successfully gapped: 2
Length of query: 72
Length of database: 17,035,801
Length adjustment: 43
Effective length of query: 29
Effective length of database: 14,790,599
Effective search space: 428927371
Effective search space used: 428927371
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)