BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0104100 Os08g0104100|AK063887
         (180 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0104100  Conserved hypothetical protein                      300   3e-82
Os05g0432500  Conserved hypothetical protein                      169   1e-42

>Os08g0104100 Conserved hypothetical protein
          Length = 180

 Score =  300 bits (769), Expect = 3e-82,   Method: Compositional matrix adjust.
 Identities = 159/180 (88%), Positives = 159/180 (88%)

Query: 1   MGDAASSASAAEMVGRAVGEVREALNEHADVVAELFGRVSSDLRSGFRPALDSFLGFFHA 60
           MGDAASSASAAEMVGRAVGEVREALNEHADVVAELFGRVSSDLRSGFRPALDSFLGFFHA
Sbjct: 1   MGDAASSASAAEMVGRAVGEVREALNEHADVVAELFGRVSSDLRSGFRPALDSFLGFFHA 60

Query: 61  VDWKEPWLISMLIFHAILLLVTIISRRNVNFQLILSALTFSGVFLAERINTFLGQHWKSF 120
           VDWKEPWLISMLIFHAILLLVTIISRRNVNFQLILSALTFSGVFLAERINTFLGQHWKSF
Sbjct: 61  VDWKEPWLISMLIFHAILLLVTIISRRNVNFQLILSALTFSGVFLAERINTFLGQHWKSF 120

Query: 121 SSQNYFDPQGLFISVVWSGPXXXXXXXXXXXXXXXXXXXXXRWKRAELRHRAREVRNKQD 180
           SSQNYFDPQGLFISVVWSGP                     RWKRAELRHRAREVRNKQD
Sbjct: 121 SSQNYFDPQGLFISVVWSGPLLLITILILVNTLVTLCLLMVRWKRAELRHRAREVRNKQD 180



>Os05g0432500 Conserved hypothetical protein
          Length = 163

 Score =  169 bits (427), Expect = 1e-42,   Method: Compositional matrix adjust.
 Identities = 78/161 (48%), Positives = 104/161 (64%)

Query: 20  EVREALNEHADVVAELFGRVSSDLRSGFRPALDSFLGFFHAVDWKEPWLISMLIFHAILL 79
           E + A+  H D V+ L   +S++LR G  PA DS L F  AVDW EPWLI +++FHA LL
Sbjct: 3   EAQAAVMAHLDQVSGLVQALSAELRRGIGPAADSLLAFVRAVDWTEPWLICLMVFHATLL 62

Query: 80  LVTIISRRNVNFQLILSALTFSGVFLAERINTFLGQHWKSFSSQNYFDPQGLFISVVWSG 139
           L  +  RRN NFQL +  L +SGV+LAE+IN+++ +HWKS +++NYFD  G+F+SVVWSG
Sbjct: 63  LTAVGLRRNANFQLFMLFLAYSGVYLAEKINSYMAEHWKSIATRNYFDRAGVFVSVVWSG 122

Query: 140 PXXXXXXXXXXXXXXXXXXXXXRWKRAELRHRAREVRNKQD 180
           P                     +WKRAELRHRA+  R+KQD
Sbjct: 123 PLIFISMVIVVSSLITLCRLMVKWKRAELRHRAQLARDKQD 163



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.137    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,765,542
Number of extensions: 152576
Number of successful extensions: 558
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 556
Number of HSP's successfully gapped: 2
Length of query: 180
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 86
Effective length of database: 12,127,685
Effective search space: 1042980910
Effective search space used: 1042980910
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 153 (63.5 bits)