BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os07g0656800 Os07g0656800|AK099536
(185 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os07g0656800 Conserved hypothetical protein 259 6e-70
Os08g0517600 SAM (and some other nucleotide) binding motif ... 102 2e-22
>Os07g0656800 Conserved hypothetical protein
Length = 185
Score = 259 bits (663), Expect = 6e-70, Method: Compositional matrix adjust.
Identities = 126/142 (88%), Positives = 126/142 (88%)
Query: 44 GMGRSDAXXXXXXXXXXXXXXXXRALSYGKAEYWDARYVEEGGAPYDWYQRYAALRPFVR 103
GMGRSDA RALSYGKAEYWDARYVEEGGAPYDWYQRYAALRPFVR
Sbjct: 44 GMGRSDAGVGGGGGGEGGGGGGGRALSYGKAEYWDARYVEEGGAPYDWYQRYAALRPFVR 103
Query: 104 RFAPPESRVLMIGCGSALMSEDMVDDGYTEIMNIDISSVVIEIMRKKHFNIPQLQCILNC 163
RFAPPESRVLMIGCGSALMSEDMVDDGYTEIMNIDISSVVIEIMRKKHFNIPQLQCILNC
Sbjct: 104 RFAPPESRVLMIGCGSALMSEDMVDDGYTEIMNIDISSVVIEIMRKKHFNIPQLQCILNC 163
Query: 164 FSILFLSPPPLKFTIHWSPYDT 185
FSILFLSPPPLKFTIHWSPYDT
Sbjct: 164 FSILFLSPPPLKFTIHWSPYDT 185
>Os08g0517600 SAM (and some other nucleotide) binding motif domain containing
protein
Length = 197
Score = 102 bits (254), Expect = 2e-22, Method: Compositional matrix adjust.
Identities = 47/91 (51%), Positives = 67/91 (73%), Gaps = 1/91 (1%)
Query: 68 ALSYGKAEYWDARYVEEGGAPYDWYQRYAALRPFVRRFAPPESRVLMIGCGSALMSEDMV 127
A +YG+A YWD RY +E G P+DWYQ+Y AL P + + R+L+ GCG+++ E+MV
Sbjct: 13 AQAYGEAWYWDERYRKEAG-PFDWYQKYPALAPLLAVYVRRHHRLLLAGCGNSVFGENMV 71
Query: 128 DDGYTEIMNIDISSVVIEIMRKKHFNIPQLQ 158
DDGY +I+NIDISSVVI+ M+KK+ + P L+
Sbjct: 72 DDGYQDIVNIDISSVVIDQMKKKYRDKPHLK 102
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.322 0.139 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,246,208
Number of extensions: 183345
Number of successful extensions: 377
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 376
Number of HSP's successfully gapped: 2
Length of query: 185
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 90
Effective length of database: 12,075,471
Effective search space: 1086792390
Effective search space used: 1086792390
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 153 (63.5 bits)