BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0637300 Os07g0637300|AY026039
         (363 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0637300  Similar to Pyruvate dehydrogenase kinase isofo...   746   0.0  
Os03g0370000  Similar to Pyruvate dehydrogenase kinase isofo...   609   e-175

>Os07g0637300 Similar to Pyruvate dehydrogenase kinase isoform 2 (EC 2.7.1.99)
          Length = 363

 Score =  746 bits (1925), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 363/363 (100%), Positives = 363/363 (100%)

Query: 1   MASEPVARAVAEEVGRWGSMKQTGVSLRYMMEFGSRPTERNLLLSAQFLQKELPIRIARR 60
           MASEPVARAVAEEVGRWGSMKQTGVSLRYMMEFGSRPTERNLLLSAQFLQKELPIRIARR
Sbjct: 1   MASEPVARAVAEEVGRWGSMKQTGVSLRYMMEFGSRPTERNLLLSAQFLQKELPIRIARR 60

Query: 61  ALELESLPFGLSRKPAILKVRDWYLDSFRDIRYFPEVRNRNDELAFTQMIKMIKVRHNNV 120
           ALELESLPFGLSRKPAILKVRDWYLDSFRDIRYFPEVRNRNDELAFTQMIKMIKVRHNNV
Sbjct: 61  ALELESLPFGLSRKPAILKVRDWYLDSFRDIRYFPEVRNRNDELAFTQMIKMIKVRHNNV 120

Query: 121 VPTMALGVQQLKNEQYRTRKIPTAFDEIHEFLDRFYMSRIGIRMLIGQHVALHDPDPEPG 180
           VPTMALGVQQLKNEQYRTRKIPTAFDEIHEFLDRFYMSRIGIRMLIGQHVALHDPDPEPG
Sbjct: 121 VPTMALGVQQLKNEQYRTRKIPTAFDEIHEFLDRFYMSRIGIRMLIGQHVALHDPDPEPG 180

Query: 181 VIGLINTELSPIQVAQAASEDARSICLREYGSAPEIDIYGDPTFTFPYVSSHLHLMLFEL 240
           VIGLINTELSPIQVAQAASEDARSICLREYGSAPEIDIYGDPTFTFPYVSSHLHLMLFEL
Sbjct: 181 VIGLINTELSPIQVAQAASEDARSICLREYGSAPEIDIYGDPTFTFPYVSSHLHLMLFEL 240

Query: 241 VKNSLRAVQERYMNSDKDVPPVRIIVADGAEDVTIKVSDEGGGIPRSGLPRIFTYLYSTA 300
           VKNSLRAVQERYMNSDKDVPPVRIIVADGAEDVTIKVSDEGGGIPRSGLPRIFTYLYSTA
Sbjct: 241 VKNSLRAVQERYMNSDKDVPPVRIIVADGAEDVTIKVSDEGGGIPRSGLPRIFTYLYSTA 300

Query: 301 KNPPDMDCPSEGVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSEE 360
           KNPPDMDCPSEGVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSEE
Sbjct: 301 KNPPDMDCPSEGVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSEE 360

Query: 361 PLP 363
           PLP
Sbjct: 361 PLP 363



>Os03g0370000 Similar to Pyruvate dehydrogenase kinase isoform 1 (EC 2.7.1.99)
          Length = 365

 Score =  609 bits (1571), Expect = e-175,   Method: Compositional matrix adjust.
 Identities = 302/360 (83%), Positives = 332/360 (92%)

Query: 3   SEPVARAVAEEVGRWGSMKQTGVSLRYMMEFGSRPTERNLLLSAQFLQKELPIRIARRAL 62
           SEP ARAVAEEV RWG M+QTGV+LRYMMEFG+RPTERNLL SAQFL++ELPIRIARRAL
Sbjct: 5   SEPAARAVAEEVARWGGMRQTGVTLRYMMEFGARPTERNLLRSAQFLRRELPIRIARRAL 64

Query: 63  ELESLPFGLSRKPAILKVRDWYLDSFRDIRYFPEVRNRNDELAFTQMIKMIKVRHNNVVP 122
           +L+SLPFGLS KPAILKVRDWYLDSFRD+R FPEVRNR+DELAFT+MIKMI+VRHNNVVP
Sbjct: 65  DLDSLPFGLSTKPAILKVRDWYLDSFRDLRCFPEVRNRDDELAFTEMIKMIRVRHNNVVP 124

Query: 123 TMALGVQQLKNEQYRTRKIPTAFDEIHEFLDRFYMSRIGIRMLIGQHVALHDPDPEPGVI 182
           TMALGV+QLK +   T+  P   DEIH+FLDRFYMSRIGIRMLIGQHVALH+PDPEPGVI
Sbjct: 125 TMALGVRQLKKDLGGTKAFPPGIDEIHQFLDRFYMSRIGIRMLIGQHVALHEPDPEPGVI 184

Query: 183 GLINTELSPIQVAQAASEDARSICLREYGSAPEIDIYGDPTFTFPYVSSHLHLMLFELVK 242
           GLI+  LSP+ VAQ A+EDAR+IC+REYGSAP+++IYGDP FTFPYV  HL LM+FELVK
Sbjct: 185 GLISKRLSPMLVAQHATEDARAICMREYGSAPDVNIYGDPDFTFPYVKLHLQLMMFELVK 244

Query: 243 NSLRAVQERYMNSDKDVPPVRIIVADGAEDVTIKVSDEGGGIPRSGLPRIFTYLYSTAKN 302
           NSLRAVQERYMNSDK  PPVRIIVADGAEDVTIK+SDEGGGIPRSGL RIFTYLYSTA+N
Sbjct: 245 NSLRAVQERYMNSDKHAPPVRIIVADGAEDVTIKISDEGGGIPRSGLSRIFTYLYSTAEN 304

Query: 303 PPDMDCPSEGVTMAGYGYGLPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSEEPL 362
           PPD+D  +EGVTMAGYGYG+PISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSEEPL
Sbjct: 305 PPDLDGRNEGVTMAGYGYGIPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSEEPL 364



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.139    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,939,794
Number of extensions: 566931
Number of successful extensions: 1030
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1030
Number of HSP's successfully gapped: 2
Length of query: 363
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 261
Effective length of database: 11,709,973
Effective search space: 3056302953
Effective search space used: 3056302953
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)