BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0577900 Os07g0577900|AK062833
         (121 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0577900  Target SNARE coiled-coil region domain contain...   204   8e-54
Os03g0187700  Target SNARE coiled-coil region domain contain...   119   4e-28

>Os07g0577900 Target SNARE coiled-coil region domain containing protein
          Length = 121

 Score =  204 bits (520), Expect = 8e-54,   Method: Compositional matrix adjust.
 Identities = 103/121 (85%), Positives = 103/121 (85%)

Query: 1   MANPLHGSGPLRSRNASSSDEIQLRIDPVHGDLDEEIDGLHSRVRLLKGVAQEINAEAKF 60
           MANPLHGSGPLRSRNASSSDEIQLRIDPVHGDLDEEIDGLHSRVRLLKGVAQEINAEAKF
Sbjct: 1   MANPLHGSGPLRSRNASSSDEIQLRIDPVHGDLDEEIDGLHSRVRLLKGVAQEINAEAKF 60

Query: 61  QNDFLSQLQMTLIKAQAGVKHNMRRMNKSIIQQGSNHXXXXXXXXXXXXXXXXXXSKFSR 120
           QNDFLSQLQMTLIKAQAGVKHNMRRMNKSIIQQGSNH                  SKFSR
Sbjct: 61  QNDFLSQLQMTLIKAQAGVKHNMRRMNKSIIQQGSNHVVHVVLFALFCFFVVYLLSKFSR 120

Query: 121 R 121
           R
Sbjct: 121 R 121



>Os03g0187700 Target SNARE coiled-coil region domain containing protein
          Length = 124

 Score =  119 bits (299), Expect = 4e-28,   Method: Compositional matrix adjust.
 Identities = 69/124 (55%), Positives = 81/124 (65%), Gaps = 3/124 (2%)

Query: 1   MANPLHGSG---PLRSRNASSSDEIQLRIDPVHGDLDEEIDGLHSRVRLLKGVAQEINAE 57
           MA+  +GS      R   +SSS EI L+IDP+  DLD+ I GL  +VR L+GVAQEI  E
Sbjct: 1   MASGAYGSREGLTARPAASSSSSEISLQIDPITADLDDHIVGLRGQVRKLRGVAQEIQTE 60

Query: 58  AKFQNDFLSQLQMTLIKAQAGVKHNMRRMNKSIIQQGSNHXXXXXXXXXXXXXXXXXXSK 117
           AK+QNDF+SQLQMTL KAQAGVK+NMRR+NK IIQ GSNH                  SK
Sbjct: 61  AKYQNDFISQLQMTLTKAQAGVKNNMRRINKKIIQNGSNHLFHVILFALVCFFLVYLVSK 120

Query: 118 FSRR 121
           FSRR
Sbjct: 121 FSRR 124



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.131    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,973,303
Number of extensions: 95303
Number of successful extensions: 356
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 356
Number of HSP's successfully gapped: 2
Length of query: 121
Length of database: 17,035,801
Length adjustment: 87
Effective length of query: 34
Effective length of database: 12,493,183
Effective search space: 424768222
Effective search space used: 424768222
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)