BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0573100 Os07g0573100|AK071285
         (345 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0573100  Similar to Adenylyl-sulfate kinase 1, chloropl...   478   e-135
Os03g0202001  Adenylylsulfate kinase domain containing protein    100   2e-21
Os11g0634800  Similar to Adenylyl-sulfate kinase 1, chloropl...    73   3e-13

>Os07g0573100 Similar to Adenylyl-sulfate kinase 1, chloroplast precursor (EC
           2.7.1.25) (APS kinase) (Adenosine-5\'phosphosulfate
           kinase) (ATP adenosine-5\'- phosphosulfate
           3\'-phosphotransferase)
          Length = 345

 Score =  478 bits (1230), Expect = e-135,   Method: Compositional matrix adjust.
 Identities = 238/271 (87%), Positives = 238/271 (87%)

Query: 75  ITAAVTGGXXXXXXXXXXXLECAGGXXXXXXXXXXXXXXXXXXXXXXTAHAGVSLVGENK 134
           ITAAVTGG           LECAGG                      TAHAGVSLVGENK
Sbjct: 75  ITAAVTGGPRRPRRRAPPPLECAGGSSSSLRRPREEEEEEEEEERSSTAHAGVSLVGENK 134

Query: 135 VLQMSSIVPKASNIFWHDCAVGQADRQKLLKQKGCVVWITGLSGSGKSTLACTLDRELHT 194
           VLQMSSIVPKASNIFWHDCAVGQADRQKLLKQKGCVVWITGLSGSGKSTLACTLDRELHT
Sbjct: 135 VLQMSSIVPKASNIFWHDCAVGQADRQKLLKQKGCVVWITGLSGSGKSTLACTLDRELHT 194

Query: 195 RGKLSYVLDGDNLRHGLNKDLGFKAEDRAENIRRVGEVAKLFADAGLVCIASFISPYRRD 254
           RGKLSYVLDGDNLRHGLNKDLGFKAEDRAENIRRVGEVAKLFADAGLVCIASFISPYRRD
Sbjct: 195 RGKLSYVLDGDNLRHGLNKDLGFKAEDRAENIRRVGEVAKLFADAGLVCIASFISPYRRD 254

Query: 255 RESCRALLSDGSFIEVFLNMPLELCESRDPKGLYKLARAGKIKGFTGIDDPYESPLNSEI 314
           RESCRALLSDGSFIEVFLNMPLELCESRDPKGLYKLARAGKIKGFTGIDDPYESPLNSEI
Sbjct: 255 RESCRALLSDGSFIEVFLNMPLELCESRDPKGLYKLARAGKIKGFTGIDDPYESPLNSEI 314

Query: 315 EIKEVDGVCPSPSDMAGQVVTYLEEKGFLHD 345
           EIKEVDGVCPSPSDMAGQVVTYLEEKGFLHD
Sbjct: 315 EIKEVDGVCPSPSDMAGQVVTYLEEKGFLHD 345



>Os03g0202001 Adenylylsulfate kinase domain containing protein
          Length = 82

 Score =  100 bits (248), Expect = 2e-21,   Method: Composition-based stats.
 Identities = 45/56 (80%), Positives = 50/56 (89%)

Query: 179 SGKSTLACTLDRELHTRGKLSYVLDGDNLRHGLNKDLGFKAEDRAENIRRVGEVAK 234
           SGKSTLAC L+RELH  G L+YVLDGDNLRHGLN+DL FKAEDRAENIRRVG+ +K
Sbjct: 14  SGKSTLACALNRELHCSGHLTYVLDGDNLRHGLNRDLSFKAEDRAENIRRVGKFSK 69



>Os11g0634800 Similar to Adenylyl-sulfate kinase 1, chloroplast precursor (EC
           2.7.1.25) (APS kinase) (Adenosine-5\'phosphosulfate
           kinase) (ATP adenosine-5\'- phosphosulfate
           3\'-phosphotransferase)
          Length = 60

 Score = 73.2 bits (178), Expect = 3e-13,   Method: Composition-based stats.
 Identities = 32/44 (72%), Positives = 37/44 (84%)

Query: 229 VGEVAKLFADAGLVCIASFISPYRRDRESCRALLSDGSFIEVFL 272
           VGEVAKLFADAGL+CI S ISPY+ DR +CR LL + SFIEV+L
Sbjct: 7   VGEVAKLFADAGLICITSLISPYKSDRSACRKLLPNSSFIEVYL 50



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,525,268
Number of extensions: 345234
Number of successful extensions: 966
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 965
Number of HSP's successfully gapped: 3
Length of query: 345
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 243
Effective length of database: 11,709,973
Effective search space: 2845523439
Effective search space used: 2845523439
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)