BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0493400 Os07g0493400|J065062C09
         (99 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0493400  3-oxo-5-alpha-steroid 4-dehydrogenase, C-termi...   206   4e-54
Os04g0576800  3-oxo-5-alpha-steroid 4-dehydrogenase, C-termi...   181   7e-47

>Os07g0493400 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal domain
          containing protein
          Length = 99

 Score =  206 bits (523), Expect = 4e-54,   Method: Compositional matrix adjust.
 Identities = 99/99 (100%), Positives = 99/99 (100%)

Query: 1  MVYFLLLLQGSLREHKDYDEYVIPCGDCFNRVSCPHYLAELVIYFGMLVASGAEDIPVWF 60
          MVYFLLLLQGSLREHKDYDEYVIPCGDCFNRVSCPHYLAELVIYFGMLVASGAEDIPVWF
Sbjct: 1  MVYFLLLLQGSLREHKDYDEYVIPCGDCFNRVSCPHYLAELVIYFGMLVASGAEDIPVWF 60

Query: 61 LFIFVITNLSFAAVETYNWYLQKFEDYPRSRYAIIPFVC 99
          LFIFVITNLSFAAVETYNWYLQKFEDYPRSRYAIIPFVC
Sbjct: 61 LFIFVITNLSFAAVETYNWYLQKFEDYPRSRYAIIPFVC 99



>Os04g0576800 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal domain containing
           protein
          Length = 354

 Score =  181 bits (460), Expect = 7e-47,   Method: Compositional matrix adjust.
 Identities = 83/90 (92%), Positives = 86/90 (95%)

Query: 10  GSLREHKDYDEYVIPCGDCFNRVSCPHYLAELVIYFGMLVASGAEDIPVWFLFIFVITNL 69
           G+LREHKD DEYVIPCGD FNRVSCPHYLAELVIYFGMLVASG EDIPVWFLF+FVITNL
Sbjct: 265 GTLREHKDSDEYVIPCGDWFNRVSCPHYLAELVIYFGMLVASGGEDIPVWFLFVFVITNL 324

Query: 70  SFAAVETYNWYLQKFEDYPRSRYAIIPFVC 99
           SFAAVET+ WYLQKFEDYPRSRYAIIPFVC
Sbjct: 325 SFAAVETHKWYLQKFEDYPRSRYAIIPFVC 354



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.332    0.148    0.493 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,258,785
Number of extensions: 112003
Number of successful extensions: 275
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 278
Number of HSP's successfully gapped: 2
Length of query: 99
Length of database: 17,035,801
Length adjustment: 68
Effective length of query: 31
Effective length of database: 13,485,249
Effective search space: 418042719
Effective search space used: 418042719
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 149 (62.0 bits)